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Journal of Integrative Agriculture  2013, Vol. 12 Issue (3): 398-405    DOI: 10.1016/S2095-3119(13)60239-7
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558
 ZHENG Yong-sheng, MA Xiao-gang, CHI De-zhao, GAO Ai-nong, LI Li-hui , LIU Wei-hua
1.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Qinghai Academy of Agriculture and Forestry, Xining 810000, P.R.China
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摘要  Spike number is one of three yield-related factors and is closely related to wheat yield. In the present study, we found that the inhibited and normal tillers of the 3558 line presented phenotypic differences at the elongation stage by morphological and anatomical analysis. We then initiated a proteomic study using two-dimensional electrophoresis (2-DE) and nanoscale liquid chromatography-high-definition tandem mass spectroscopy, to isolate and identify the key proteins and metabolic pathways related to spike-development inhibition. A total of 31 differentially expressed proteins (DEPs), which were mainly involved in cell cycle regulation, photosynthesis, glycolysis, stress response, and oxidation-reduction reactions, were isolated and identified. 14-3-3-like proteins and proliferating cell nuclear antigen (PCNA), involved in cell-cycle regulation, were dramatically down-regulated in inhibited tillers compared to normal tillers. Six spots corresponding to degraded Rubisco large subunits, involved in photosynthesis, were detected in different locations of the 2-DE gels and were up-regulated in inhibited tillers. In addition, the relative levels of DEPs involved in glycolysis and oxidationreduction reactions changed dramatically. Development was blocked or delayed at the elongation stage in the inhibited tillers of 3558. Weakened energy metabolism might be one reason that the inhibited tillers could not joint and develop into spikes. These DEPs and related metabolic pathways are significant for understanding the mechanism of spike-development inhibition and studying the spike-development process in wheat.

Abstract  Spike number is one of three yield-related factors and is closely related to wheat yield. In the present study, we found that the inhibited and normal tillers of the 3558 line presented phenotypic differences at the elongation stage by morphological and anatomical analysis. We then initiated a proteomic study using two-dimensional electrophoresis (2-DE) and nanoscale liquid chromatography-high-definition tandem mass spectroscopy, to isolate and identify the key proteins and metabolic pathways related to spike-development inhibition. A total of 31 differentially expressed proteins (DEPs), which were mainly involved in cell cycle regulation, photosynthesis, glycolysis, stress response, and oxidation-reduction reactions, were isolated and identified. 14-3-3-like proteins and proliferating cell nuclear antigen (PCNA), involved in cell-cycle regulation, were dramatically down-regulated in inhibited tillers compared to normal tillers. Six spots corresponding to degraded Rubisco large subunits, involved in photosynthesis, were detected in different locations of the 2-DE gels and were up-regulated in inhibited tillers. In addition, the relative levels of DEPs involved in glycolysis and oxidationreduction reactions changed dramatically. Development was blocked or delayed at the elongation stage in the inhibited tillers of 3558. Weakened energy metabolism might be one reason that the inhibited tillers could not joint and develop into spikes. These DEPs and related metabolic pathways are significant for understanding the mechanism of spike-development inhibition and studying the spike-development process in wheat.
Keywords:  wheat       spike-development inhibition       2-DE       differentially expressed proteins  
Received: 02 May 2012   Accepted:
Fund: 

This work was supported by the National High Technology Research and Development Program of China (2011AA100102 and 2006AA10Z174).

Corresponding Authors:  Correspondance LIU Wei-hua, Tel: +86-10-62176077, Fax: +86-10-62189650, E-mail: liuweihua@caas.cn   
About author:  ZHENG Yong-sheng, Tel: +86-531-83178713, E-mail: zhengyongsheng123@163.com;

Cite this article: 

ZHENG Yong-sheng, MA Xiao-gang, CHI De-zhao, GAO Ai-nong, LI Li-hui , LIU Wei-hua. 2013. Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558. Journal of Integrative Agriculture, 12(3): 398-405.

[1]Carlioz A, Touati D. 1986. Isolation of superoxide dismutasemutants in Escherichia coli: is superoxide dismutasenecessary for aerobic life? The EMBO Journal, 5, 623-630

[2]Cui S, Huang F, Wang J, Ma X, Cheng Y, Liu J. 2005. Aproteomic analysis of cold stress responses in riceseedlings. Proteomics, 5, 3162-3172

[3]Gary R, Ludwig D, Cornelius H, MacInnes M, Park M. 1997.The DNA repair endonuclease XPG binds toproliferating cell nuclear antigen (PCNA) and sharessequence elements with the PCNA-binding regions ofFEN-1 and cyclin-dependent kinase inhibitor p21Journal of Biological Chemistry, 272, 24522-24529

[4]Gravois K, McNew R. 1993. Genetic relationships amongand selection for rice yield and yield components. CropScience, 33, 249-252

[5]Hanada K. 1993. Science of the Rice Plant. vol. 1Morphology. Food and Agriculture Policy ResearchCenter, Tokyo. pp. 222-258

[6]Ishida S, Fukazawa J, Yuasa T, Takahashi Y. 2004.Involvement of 14-3-3 signaling protein binding in thefunctional regulation of the transcriptional activatorREPRESSION OF SHOOT GROWTH? by gibberellins The Plant Cell, 16, 2641-2651

[7]Ishida S, Yuasa T, Nakata M, Takahashi Y. 2008. A tobaccocalcium-dependent protein kinase, CDPK1, regulatesthe transcription factor REPRESSION OF SHOOTGROWTH in response to gibberellins. The Plant Cell,20, 3273-3288

[8]Kuraparthy V, Sood S, Dhaliwal H, Chhuneja P, Gill B. 2007.Identification and mapping of a tiller inhibition gene(tin3) in wheat. Theoretical and Applied Genetics, 114,285-294

[9]Li X, Qian Q, Fu Z, Wang Y, Xiong G, Zeng D, Wang X, LiuX, Teng S, Hiroshi F. 2003. Control of tillering in rice.Nature, 422, 618-621

[10]Orr W, Sohal R. 1994. Extension of life-span byoverexpression of superoxide dismutase and catalasein Drosophila melanogaster. Science, 263, 1128-1130

[11]Peng Z, Yen C, Yang J. 1998. Genetic control of oligo-culmsin common wheat (Triticum aestivum). WheatInformation Service, 26, 19-24

[12]Pignocchi C, Doonan J H. 2011. Interaction of a 14-3-3protein with the plant microtubule-associated proteinEDE1 Annals of Botany, 107, 1103-1109.

[13]Pignocchi C, Minns G E, Nesi N, Koumproglou R, KitsiosG, Benning C, Lloyd C W, Doonan J H, Hills M J. 2009.ENDOSPERM DEFECTIVE1 is a novel microtubuleassociatedprotein essential for seed development inArabidopsis. The Plant Cell, 21, 90-105

[14]Rakwal R, Agrawal G, Yonekura M. 1999. Separation ofproteins from stressed rice (Oryza sativa L.) leaf tissuesby two-dimensional polyacrylamide gel electrophoresis:induction of pathogenesis-related and cellularprotectant proteins by jasmonic acid, UV irradiationand copper chloride. Electrophoresis, 20, 3472-3478

[15]Richards R. 1988. A tiller inhibitor gene in wheat and itseffect on plant growth. Australian Journal of Agricultural Research, 39, 749-757

[16]Ryu H, Cho H, Kim K, Hwang I. 2010. Phosphorylationdependent nucleocytoplasmic shuttling of BES1 is akey regulatory event in brassinosteroid signaling.Molecules and Cells, 29, 283-290

[17]Shen Z, Li P, Ni R, Ritchie M, Yang C, Liu G, Ma W, Liu G,Ma L, Li S. 2009. Label-free quantitative proteomicsanalysis of etiolated maize seedling leaves duringgreening. Molecular & Cellular Proteomics, 8, 2443.

[18]Spielmeyer W, Richards R. 2004. Comparative mapping ofwheat chromosome 1AS which contains the tillerinhibition gene (tin) with rice chromosome 5S.Theoretical and Applied Genetics, 109, 1303-1310

[19]Wan X, Liu J. 2008. Comparative proteomics analysisreveals an intimate protein network provoked byhydrogen peroxide stress in rice seedling leaves.Molecular & Cellular Proteomics, 7, 1469.

[20]Wijngaard P W J, Sinnige M P, Roobeek I, Reumer A,Schoonheim P J, Mol J N M, Wang M, De Boer A H.2005. Abscisic acid and 14-3-3 proteins control K+channel activity in barley embryonic root

[21]The PlantJournal, 41, 43-55

[22]Yan S P, Zhang Q Y, Tang Z C, Su W A, Sun W N. 2006.Comparative proteomic analysis provides new insightsinto chilling stress responses in rice. Molecular &Cellular Proteomics, 5, 484-496

[23]Yao Y, Yang Y, Liu J. 2006. An efficient protein preparationfor proteomic analysis of developing cotton fibers by2-DE. Electrophoresis, 27, 4559-4569

[24]Zhang J P, Wu J, Liu W H, Lu X, Yang X M, Gao A N, Li XQ, Lu Y Q, Li L H. 2012. Genetic mapping of a fertile tillerinhibition gene, ftin, in wheat. Molecular Breeding, doi:10.1007/s11032-012-9801-0.
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