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| Comparative Proteomic Analysis of Wheat (Triticum aestivum L.) Hybrid Necrosis |
| JIANG Qi-yan, HU Zheng, PAN Xing-lai , ZHANG Hui |
1.National Key Facilities for Crop Genetic Resources and Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Food Crop Science Department, Cotton Research Institute, Shanxi Academy of Agriculture Sciences, Yuncheng 044000, P.R.China |
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摘要 Hybrid necrosis is the gradual premature death of leaves or plants in certain F1 hybrids of wheat (Triticum aestivum L.). Comparison of protein expression in necrotic and normal wheat leaves showed that the abundance of 33 proteins was changed significantly, and 24 of these proteins were identified. These proteins were involved in plant growth and development, antioxidation, photosynthesis and carbon assimilation, amino acid and protein biosynthesis, cytological signal transduction, DNA and RNA modification, protein transport, folding and assembly according to their functions. The down-regulation of uroporphyrinogen decarboxylase and the up-regulation of lipoxygenases in necrotic leaves may be related to the oxidative stress in the necrotic cells. The heat shock proteins may play the cytoprotective role. The differential expression of photosynthesis and carbon assimilation related proteins indicated chlorophyll biosynthesis and chloroplast development were inhibited and might finally cause the gradual chlorosis and cell death in necrotic leaves. The results of this study give a comprehensive picture of the post-transcriptional response to necrosis in hybrid wheat leaves and serve as a platform for further characterization of gene function and regulation in wheat hybrid necrosis.
Abstract Hybrid necrosis is the gradual premature death of leaves or plants in certain F1 hybrids of wheat (Triticum aestivum L.). Comparison of protein expression in necrotic and normal wheat leaves showed that the abundance of 33 proteins was changed significantly, and 24 of these proteins were identified. These proteins were involved in plant growth and development, antioxidation, photosynthesis and carbon assimilation, amino acid and protein biosynthesis, cytological signal transduction, DNA and RNA modification, protein transport, folding and assembly according to their functions. The down-regulation of uroporphyrinogen decarboxylase and the up-regulation of lipoxygenases in necrotic leaves may be related to the oxidative stress in the necrotic cells. The heat shock proteins may play the cytoprotective role. The differential expression of photosynthesis and carbon assimilation related proteins indicated chlorophyll biosynthesis and chloroplast development were inhibited and might finally cause the gradual chlorosis and cell death in necrotic leaves. The results of this study give a comprehensive picture of the post-transcriptional response to necrosis in hybrid wheat leaves and serve as a platform for further characterization of gene function and regulation in wheat hybrid necrosis.
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Received: 13 April 2012
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (30440047) and the Program for Germplasm Innovation and Utilization from the Ministry of Agriculture of China (NB2010-2130135). |
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Corresponding Authors:
Correspondence ZHANG Hui, Tel: +86-10-82108746, Fax: +86-10-62816649, E-mail: zhanghui06@caas.cn
E-mail: Correspondence ZHANG Hui, Tel: +86-10-82108746, Fax: +86-10-62816649, E-mail: zhanghui06@caas.cn
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Cite this article:
JIANG Qi-yan, HU Zheng, PAN Xing-lai , ZHANG Hui.
2013.
Comparative Proteomic Analysis of Wheat (Triticum aestivum L.) Hybrid Necrosis. Journal of Integrative Agriculture, 12(3): 387-397.
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| [1]Andrews H K, Zhang Y Q, Trotta N, Broadie K. 2002.Drosophila Sec10 is required for hormone secretionbut not general exocytosis or neurotransmission.Traffic, 3, 906-921[2]Bando Y, Katayama T, Kasai K, Taniguchi M, Tohyama M.2003. GRP94 (94 kDa glucose-regulated protein)suppresses ischemic neuronal cell death againstischemia/reperfusion injury. European Journal ofNeuroscience, 18, 829-840[3]Bijral J S, Gupta B B, Singh B, Sharma T R, Kanwal K S,Gupta S C. 1990. Hybrid necrosis in wheat. IndianJournal of Genetics & Plant Breeding, 50, 189-191[4]Bomblies K, Weigel D. 2007. Hybrid necrosis: autoimmunityas a potential gene-flow barrier in plant species. NatureReviews Genetics, 8, 382-393[5]Caldwell R M, Compton L E. 1943. Complementary lethalgenes in wheat causing a progressive lethal necrosisof seedlings. Journal of Heredity, 34, 67-70[6]Callard D, Axelos M, Mazzolini L. 1996. Novel molecularmarkers for late phases of the growth cycle ofArabidopsis thaliana cell-suspension cultures areexpressed during organ senescence. Plant Physiology,112, 705-715[7]Chase J W, Williams K R. 1986. Single-stranded DNA bindingproteins required for DNA replication. Annual Reviewof Biochemistry, 55, 103-136[8]Chen X X, Ding Y, Liu C G, Mikhail S, Yang C S. 2002.Overexpression of glucose-regulated protein 94 (Grp94)in esophageal adenocarcinomas of a rat surgical modeland humans. Carcinogenesis, 23, 123-130[9]Chu C G, Faris J D, Friesen T L, Xu S S. 2006. Molecularmapping of hybrid necrosis genes Ne1 and Ne2 inhexaploid wheat using microsatellite markers.Theoretical and Applied Genetics, 112, 1374-1381[10]Collinge D B, Kragh K M, Mikkelsen J D, Nielsen K K,Rasmussen U, Vad K. 1993. Plant chitinases. The PlantJournal, 3, 31-40[11]Dalal M, Khanna-Chopra R. 2001. Differential response ofantioxidant enzymes in leaves of necrotic wheat hybrids and quantitative proteomic analysis of clinicalhepatocellular carcinoma using laser capturemicrodissection coupled with isotope-coded affinity tagand two-dimensional liquid chromatography massspectrometry. Molecular Cell Proteomics, 3, 399-409[12]Lin B Y. 1984. Ploidy barrier to endosperm development inmaize. Genetics, 107, 103-115[13]Maccarrone M, Melino G, Finazzi-Agrò A. 2001. Plantlipoxygenases and their involvement in programmedcell death. Cell Death and Differentiation, 267, 5078-5084[14]Maccarrone M, Zadelhoff G V, Veldink G A, Vliegenthart JF G, Finazzi-Agrò A. 2000. Early activation oflipoxygenase in lentil (Lens culinaris) root protoplastsby oxidative stress induces programmed cell death.European Journal of Biochemistry, 267, 5078-5084[15]Matsuoka Y, Takumi S, Kawahara T. 2007. Natural variationfor triploid F1 hybrid formation in allohexaploid wheatspeciation. Theoretical and Applied Genetics, 115, 509-518[16]Michalak M, Corbett E F, Measeli N, Nakamura K, Opas M.1999. Calreticulin : one protein, one gene, manyfunctions. Biochemical Journal, 344, 281-292[17]Miernyk J A. 1999. Protein folding in the plant cell. PlantPhysiology, 121, 695-703[18]Mizuno N, Hosogi N, Park P, Takumi S. 2010. Hypersensitiveresponse-like reaction is associated with hybridnecrosis in interspecific crosses between tetraploidwheat and Aegilops tauschii Coss. PLoS ONE, 5,e11326.Mizuno N, Shitsukawa N, Hosogi N, Park P, Takumi S. 2011.Autoimmune response and repression of mitotic celldivision occur in inter-specific crosses betweentetraploid wheat and Aegilops tauschii Coss that showlow temperature-induced hybrid necrosis. The PlantJournal, 68, 114-128[19]Mock H P, Grimm B. 1997. Reduction of uroporphyrinogendecarboxylase by antisense RNA expression affectsactivities of other enzymes involved in tetrapyrrolebiosynthesis and leads to light-dependent necrosis.Plant Physiology, 113, 1101-1112[20]Nishikawa K, Mori T, Takami N, Furuta Y. 1974. Mappingof progressive necrosis genes, Ne1 and Ne2 of commonwheat by the telocentric method. Japanese Journal ofBreeding, 24, 277-281[21]Novick P, Guo W. 2002. Ras family therapy: Rab, Rho andRal talk to the exocyst. Trends in Cell Biology, 12, 247-249[22]Pan X L, Jiang Q Y, Pan Q Y, Wen X F, Shi Y H, Wang Y J,Pan T Y, Xie S G, Zhang G Y, Wu S J, et al. 2009.Proteomic analysis of ‘hybrid necrosis’ in wheat(Triticum aestivum) leaves. Functional Plant Biology,36, 251-259[23]Pappin D J. 1997. Peptide mass fingerprinting using MALDITOFmass spectrometry. Methods Molecular Biology,64, 165-173[24]Passarinho P A, van Hengel A J, Fransz P F, de Vries S C.2001. Expression pattern of the Arabidopsis thalianaAtEP3/AtchitIV endochitinase gene. Planta, 212, 556-567[25]Peltier G, Cournac L. 2002. Chlororespiration. AnnualReview of Plant Biology, 53, 523-550[26]Perkins D N, Pappin D J, Creasy D M, Cottrell J S. 1999.Probability-based protein identification by searchingsequence databases using mass spectrometry data.Electrophoresis, 20, 3551-3567[27]Portis A R. 1995. The regulation of Rubisco by Rubiscoactivase. Journal of Experimental Botany, 46, 1285-1291[28]Portis A R. 2003. Rubisco activase - Rubisco’s catalyticchaperone. Photosynthesis Research, 75, 11-12[29]Regalado A P, Pinheiro C, Vidal S, Chaves I, Ricardo C P P.2000. Rodrigues-Pousada C. The lupinus albus class-III chitinase gene, IF3, is constitutively expressed invegetative organs and developing seeds. Planta, 210,543-550[30]Sagi G, Katz A, Guenoune-Gelbart D, Epel B L. 2005. Class1 reversibly glycosylated polypeptides areplasmodesmal-associated proteins delivered toplasmodesmata via the Golgi apparatus. The Plant Cell,17, 1788-1800[31]Salvucci M E, Ogren W L. 1996. The mechanism of Rubiscoactivase: insights from studies of the properties andstructure of the enzyme. Photosynthesis Research, 47,1-11[32]Schirmer E C, Glover J R, Singer M A, Lindquist S. 1996.HSP100/Clp proteins: a common mechanism explainsdiverse functions. Trends in Biochemical Sciences, 21,289-296[33]Sharma G, Prabhu K V, Khanna-Chopra R. 2004. Decline inreactive oxygen species at low light intensity canovercome necrosis barrier in hybrid wheat. CurrentScience, 87, 500-503[34]Sharma G, Srivalli B, Khanna-Chopra R. 2003. Hybridnecrosis in wheat - A genetic system showing reducedcapacity to detoxify reactive oxygen species leading toprogrammed cell death. Indian Journal ofBiotechnology, 2, 17-25[35]Shen S H, Jing Y X, Kuang T Y. 2003. Proteomics approachto identify wound - response related proteins from riceleaf sheath. Proteomics, 3, 527-535[36]Souer E, van Houwelingen A, Kloos D, Mol J, Koes R.1996. The no apical meristem gene of petunia is requiredfor pattern formation in embryos and flowers and isexpressed at meristem and primordia boundaries. Cell,85, 159-170[37]Sreedhar A S, Csermely P. 2004. Heat shock proteins in theregulation of apoptosis: new strategies in tumor therapy:a comprehensive review. Pharmacology andTherapeutics, 101, 227-257[38]Sweeney G D. 1986. Porphyria cutanea tarda, or theuroporphyrinogen decarboxylase deficiency diseases. Clinical Biochemistry, 19, 3-15[39]Takase H, Minami M, Meshi T, Iwabuchi M. 1995. Theelectronic plant gene register. Plant Physiology, 109,337-339[40]Tamura K, Adachi Y, Chiba K, Oguchi K, Takahashi H.2002. Identification of Ku70 and Ku80 homologues inArabidopsis thaliana: evidence for a role in the repairof DNA double-strand breaks. The Plant Journal, 29,771-781[41]Tanaka R, Tanaka A. 2007. Tetrapyrrole biosynthesis inhigher plants. Annual Review of Plant Biology, 58, 21-46[42]Torres-Roca J F, Lecoeur H, Amatore C, Gougeon M L.1995. The early intracellular production of reactiveoxygen intermediate mediates apoptosis indexamethasone-treated thymocytes. Cell Death andDifferentiation, 2, 309-319[43]Wang Z Y, Portis A R. 1992. Dissociation of ribulose-1,5-bisphosphate bound to ribulose-1,5-bisphosphatecarboxylase/oxygenase and its enhancement byribulose-1,5-bisphosphate carboxylase/oxygenaseactivase-mediated hydrolysis of ATP[44]PlantPhysiology, 99, 1348-1353[45]Yang P F, Liang Y, Shen S H, Kuang T Y. 2006. Proteomeanalysis of rice uppermost internodes at the milky stage.Proteomics, 6, 1-9[46]Zapata J M, Sabater B, Martín M. 1998. Identification of athylakoid peroxidase of barley which oxidizeshydroquinone. Phytochemistry, 48, 1119-1123[47]Zavaliev R, Sagi G, Gera A, Epel B L. 2010. The constitutiveexpression of Arabidopsis plasmodesmal associatedclass 1 reversibly glycosylated polypeptide impairsplant development and virus spread. Journal ofExperimental Botany, 61, 131-142[48]Zeven A C. 1981. Eighth supplementary list of wheatvarieties classified according to their genotype forhybrid necrosis. Euphytica, 30, 512-539. |
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