Please wait a minute...
Journal of Integrative Agriculture  2012, Vol. 12 Issue (7): 1167-1172    DOI: 10.1016/S1671-2927(00)8643
ANIMAL SCIENCE · VETERINARY SCIENCE Advanced Online Publication | Current Issue | Archive | Adv Search |
Effects of Dietary Energy Level on the Expression of the HSL Gene in Different Tissues of Sheep
 ZHANG Ying-jie, LIU Yue-qin, CHENG Shan-yan,  SONG Jie
College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  A total of 36 four-mon-old hybrid lambs (Dorset×Thin-tailed Han sheep) with similar body weight (BW) were randomly allocated to three dietary treatments with different energy (7.21, 10.33 and 13.49 MJ d-1 ME) but similar protein levels. The animals were slaughtered and subcutaneous fat, longissimus dorsi muscle, femoral biceps muscle and cardiac muscle tissue samples were taken after being treated for 40 d. The samples were then subjected to quantitative PCR to determine mRNA expression of hormone-sensitive lipase (HSL) in different tissues in the laboratory. The findings showed that the abundance of HSL mRNA decreased with the elevation of dietary energy. In the subcutaneous fatty tissue, the HSL mRNA levels showed significant differences among the three groups (P<0.01); in the longissimus dorsi and femoral biceps muscles, the HSL mRNA level in the low energy group was significantly higher than that in the moderate and high energy groups (P<0.01). In the cardiac muscle, the HSL mRNA level in the moderate energy group was significantly different from the low and high energy groups (P<0.05). The number of HSL copies (Qty) in different tissues of sheep was different, it was greater in the subcutaneous fat than in longissimus dorsi muscle, femoral biceps muscle and heart.

Abstract  A total of 36 four-mon-old hybrid lambs (Dorset×Thin-tailed Han sheep) with similar body weight (BW) were randomly allocated to three dietary treatments with different energy (7.21, 10.33 and 13.49 MJ d-1 ME) but similar protein levels. The animals were slaughtered and subcutaneous fat, longissimus dorsi muscle, femoral biceps muscle and cardiac muscle tissue samples were taken after being treated for 40 d. The samples were then subjected to quantitative PCR to determine mRNA expression of hormone-sensitive lipase (HSL) in different tissues in the laboratory. The findings showed that the abundance of HSL mRNA decreased with the elevation of dietary energy. In the subcutaneous fatty tissue, the HSL mRNA levels showed significant differences among the three groups (P<0.01); in the longissimus dorsi and femoral biceps muscles, the HSL mRNA level in the low energy group was significantly higher than that in the moderate and high energy groups (P<0.01). In the cardiac muscle, the HSL mRNA level in the moderate energy group was significantly different from the low and high energy groups (P<0.05). The number of HSL copies (Qty) in different tissues of sheep was different, it was greater in the subcutaneous fat than in longissimus dorsi muscle, femoral biceps muscle and heart.
Keywords:  sheep      energy level      hormone-sensitive lipase (HSL)      gene expression  
Received: 25 February 2011   Accepted: 27 July 2012
Fund: 

The present study is supported by China Agriculture Research System-Mutton Sheep (CARS-39).

Corresponding Authors:  ZHANG Ying-jie, Tel: +86-312-7528886, E-mail: zhangyingjie66@126.com     E-mail:  zhangyingjie66@126.com

Cite this article: 

ZHANG Ying-jie, LIU Yue-qin, CHENG Shan-yan, SONG Jie. 2012. Effects of Dietary Energy Level on the Expression of the HSL Gene in Different Tissues of Sheep. Journal of Integrative Agriculture, 12(7): 1167-1172.

[1]Awad A B, Chattopadhyay J P. 1986. Effect of dietary saturated fatty acids on hormone-sensitive lipolysis in rat adipocytes. Journal of Nutrition, 116, 1088-1094.

[2]Berraondo B, Martínez J A. 2000. Free fatty acids are involved in the inverse relationship between hormonesensitive lipase (HSL) activity and expression in adipose tissue after high-fat feeding or β3-adrenergic stimulation. Obesity Research, 8, 255-261.

[3]Haemmerle G, Zimmermann R, Strauss J G, Kratky D, Riederer M, Knipping G, Zechner R. 2002. Hormone-sensitive lipase deficiency in mice changes the plasma lipid profile by affecting the tissue-specific expression pattern of lipoprotein lipase in adipose tissue and muscle. Journal of Biological Chemistry, 277, 12946-12952.

[4]Hansson O, Donsmark M, Ling C, Nevsten P, Danfelter M, Andersen J L, Galbo H, Holm C. 2005. Transcriptome and proteome analysis of soleus muscle of hormonesensitive lipase-null mice. Journal of Lipid Research, 46, 2614-2623.

[5]Harada K, Shen W J, Patel S, Natu V, Wang J, Osuga J, Ishibashi S, Kraemer F B. 2003. Resistance to high-fat diet-induced obesity and altered expression of adiposespecific genes in HSL-deficient mice. American Journal of Physiology Endocrinology and Metabolism, 285, 1182-1195.

[6]Hodgson R R, Davis G W, Smith G C, Savell J W, Cross H R. 1991. Relationships between pork loin palatability traits and physical characteristics of cooked chops. Journal of Animal Science, 69, 4858-4865.

[7]Holm C, Kirchgessner T G, Svenson K L, Fredrikson G, Nilsson S, Miller C G, Shively J E, Heinzmann C, Sparkes R S, Mohandas T. 1988. Hormone-sensitive lipase: sequence, expression and chromosomal localization to 19 cent-q13.3. Science, 241, 1503-1506.

[8]Langfort J, Ploug T, Ihlemann J, Saldo M, Holm C, Galbo H. 1999. Expression of hormone-sensitive lipase and its regulation by adrenaline in skeletal muscle. The Biochemical Journal, 340, 459-465.

[9]Langfort J, Ploug T, Ihlemann J, Holm C, Galbo H. 2000. Stimulation of hormone-sensitive lipase activity by contractions in rat skeletal muscle. The Biochemical Journal, 351, 207-214.

[10]Liu Z H, Yang F Y, Kong L J, Zhou X R, Gu Y H, Wang X Y. 2007. Effects of dietary energy level on the content of intramuscular fat and mRNA expression for fatty acid synthase and hormone-sensitive lipase in growingfinishing pigs. Chinese Journal of Animal and Veterinary Science, 38, 934-941. (in Chinese)

[11]Lobo M V, Huerta L, Arenas M I, Busto R, Lasunción M A, Martín-Hidalgo A. 2009. Hormone-sensitive lipase expression and IHC localization in the rat ovary, oviduct, and uterus. Journal of Histochemistry and Cytochemistry, 57, 51-60.

[12]National Research Council. 1985. Nutrient Requirements of Sheep. National Academy Press, Washington, D.C. Qiao Y, Huang Z, Li Q, Liu Z, Hao C, Shi G, Dai R, Xie Z. 2007. Developmental changes of the FAS and HSL mRNA expression and their effects on the content of intramuscular fat in Kazak and Xinjiang Sheep. Journal of Genetics and Genomics, 34, 909-917.

[13]Reid B N, Ables G P, Otlivanchik O A, Schoiswohl G, Zechner R, Blaner W S, Goldberg I J, Schwabe R F, Chua Jr S C, Huang L S. 2008. Hepatic overexpression of hormonesensitive lipase and adipose triglyceride lipase promotes fatty acid oxidation, stimulates direct release of free fatty acids, and ameliorates steatosis. Journal of Biological Chemistry, 283, 13087-13099.

[14]Shimada M, Ishibashi S, Yamamoto K, Kawamura M, Watanabe Y, Gotoda T, Harada K, Inaba T, Ohsuga J, Yazaki Y. 1995. Overexpression of human lipoprotein lipase increases hormone-sensitive lipase activity in adipose tissue of mice. Biochemical and Biophysical Research Communications, 211, 761-766.

[15]Stich V, Harant I, de Glisezinski I, Crampes F, Berlan M, Kunesova M, Hainer V, Dauzats M, Rivière D, Garrigues M, et al. 1997. Adipose tissue lipolysis and hormonesensitive lipase expression during very-low-calorie diet in obese female identical twins. Journal of Clinical Endocrinology and Metabolism, 82, 739-744.

[16]Sztalryd C, Kraemer F B. 1994. Regulation of hormonesensitive lipase during fasting. Journal of Physiology, 266, 179-185.

[17]Watt M J, Heigenhauser G J, Spriet L L. 2003. Effects of dynamic exercise intensity on the activation of hormone-sensitive lipase in human skeletal muscle. Journal of Physiology, 547, 301-308.

[18]Wang Y H, Byrne K A, Reverter A, Harper G S, Taniguchi M, McWilliam S M, Mannen H, Oyama K, Lehnert S A. 2005. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle. Mammalian Genome, 16, 201-210.
[1] YIN Xue-jiao, JI Shou-kun, DUAN Chun-hui, TIAN Pei-zhi, JU Si-si, YAN Hui, ZHANG Ying-jie, LIU Yue-qin. The succession of fecal bacterial community and its correlation with the changes of serum immune indicators in lambs from birth to 4 months[J]. >Journal of Integrative Agriculture, 2023, 22(2): 537-550.
[2] ZHANG Yan-mei, AO De, LEI Kai-wen, XI Lin, Jerry W SPEARS, SHI Hai-tao, HUANG Yan-ling, YANG Fa-long. Dietary copper supplementation modulates performance and lipid metabolism in meat goat kids[J]. >Journal of Integrative Agriculture, 2023, 22(1): 214-221.
[3] JIANG Yong, MA Xin-yan, XIE Ming, ZHOU Zheng-kui, TANG Jing, CHANG Guo-bin, CHEN Guo-hong, HOU Shui-sheng. Dietary threonine deficiency affects expression of genes involved in lipid metabolism in adipose tissues of Pekin ducks in a genotype-dependent manner[J]. >Journal of Integrative Agriculture, 2022, 21(9): 2691-2699.
[4] RONG Hao, YANG Wen-jing, XIE Tao, WANG Yue, WANG Xia-qin, JIANG Jin-jin, WANG You-ping. Transcriptional profiling between yellow- and black-seeded Brassica napus reveals molecular modulations on flavonoid and fatty acid content[J]. >Journal of Integrative Agriculture, 2022, 21(8): 2211-2226.
[5] AN Feng, ZHANG Kang, ZHANG Ling-kui, LI Xing, CHEN Shu-min, WANG Hua-sen, CHENG Feng. Genome-wide identification, evolutionary selection, and genetic variation of DNA methylation-related genes in Brassica rapa and Brassica oleracea[J]. >Journal of Integrative Agriculture, 2022, 21(6): 1620-1632.
[6] FAN Xiao-xue, BIAN Zhong-hua, SONG Bo, XU Hai. Transcriptome analysis reveals the differential regulatory effects of red and blue light on nitrate metabolism in pakchoi (Brassica campestris L.)[J]. >Journal of Integrative Agriculture, 2022, 21(4): 1015-1027.
[7] LIU Cong, LI De-xiong, HUANG Xian-biao, Zhang Fu-qiong, Xie Zong-zhou, Zhang Hong-yan, Liu Ji-hong. Manual thinning increases fruit size and sugar content of Citrus reticulata Blanco and affects hormone synthesis and sugar transporter activity[J]. >Journal of Integrative Agriculture, 2022, 21(3): 725-735.
[8] YIN Xue-jiao, JI Shou-kun, DUAN Chun-hui, TIAN Pei-zhi, JU Si-si, YAN Hui, ZHANG Ying-jie, LIU Yue-qin. Dynamic change of fungal community in the gastrointestinal tract of growing lambs[J]. >Journal of Integrative Agriculture, 2022, 21(11): 3314-3328.
[9] DUAN Yao-ke, HAN Rong, SU Yan, WANG Ai-ying, LI Shuang, SUN Hao, GONG Hai-jun. Transcriptional search to identify and assess reference genes for expression analysis in Solanum lycopersicum under stress and hormone treatment conditions[J]. >Journal of Integrative Agriculture, 2022, 21(11): 3216-3229.
[10] Kashif NOOR, Hafiza Masooma Naseer CHEEMA, Asif Ali KHAN, Rao Sohail Ahmad KHAN. Expression profiling of transgenes (Cry1Ac and Cry2A) in cotton genotypes under different genetic backgrounds[J]. >Journal of Integrative Agriculture, 2022, 21(10): 2818-2832.
[11] MA Tao, DENG Kai-dong, TU Yan, ZHANG Nai-feng, ZHAO Qi-nan, LI Chang-qing, JIN Hai, DIAO Qi-yu. Recent advances in nutrient requirements of meat-type sheep in China: A review[J]. >Journal of Integrative Agriculture, 2022, 21(1): 1-14.
[12] WANG Pei-pei, WANG Zhao-ke, GUAN Le, Muhammad Salman HAIDER, Maazullah NASIM, YUAN Yong-bing, LIU Geng-sen, LENG Xiang-peng. Versatile physiological functions of the Nudix hydrolase family in berry development and stress response in grapevine[J]. >Journal of Integrative Agriculture, 2022, 21(1): 91-112.
[13] GUO Bing-bing, LI Jia-ming, LIU Xing, QIAO Xin, Musana Rwalinda FABRICE, WANG Peng, ZHANG Shao-ling, WU Ju-you. Identification and expression analysis of the PbrMLO gene family in pear, and functional verification of PbrMLO23[J]. >Journal of Integrative Agriculture, 2021, 20(9): 2410-2423.
[14] JI Xiao-hao, WANG Bao-liang, WANG Xiao-di, WANG Xiao-long, LIU Feng-zhi, WANG Hai-bo. Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes[J]. >Journal of Integrative Agriculture, 2021, 20(6): 1525-1539.
[15] CHEN Chang-long, YUAN Fang, LI Xiao-ying, MA Rong-cai, XIE Hua. Jasmonic acid and ethylene signaling pathways participate in the defense response of Chinese cabbage to Pectobacterium carotovorum infection[J]. >Journal of Integrative Agriculture, 2021, 20(5): 1314-1326.
No Suggested Reading articles found!