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| The Dynamics of Changes in Starch and Lipid Droplets and Sub-Cellular Localization of β-Amylase During the Growth of Lily Bulbs |
| WU Sha-sha, WU Jin-di, JIAO Xue-hui, ZHANG Qi-xiang, , LV Ying-min |
1.College of Landscape Architecture, Beijing Forestry University, Beijing 100083, P.R.China
2.China National Research Center for Floriculture Engineering & Technology, Beijing 100083, P.R.China |
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摘要 The ultra-structure of mother and outer daughter scales of Lilium Oriental hybrid Sorbonne were studied using transmission electron microscope to examine the sub-cellular localization of starch and lipid droplets during growth and development from shoot emergence to senescence. The contents of starch granules and lipid droplets in the cell of the mother scales decreased significantly from shoot emergence to anthesis, indicating that these scales served as a source for growth and development. After flowering, the number of starch granules and lipid droplets increased dramatically, and finally the cells were filled with the above molecules indicating that the bulb becomes a major sink during bulb enlargement. Ultrastructure observation also showed that symplastic pathway is the main pathway in cells in the exchange and transportation of material during bulb development. The activity of β-amylase, one of the key enzymes catalyzing starch breakdown, showed a similar trend. The enzyme sub-cellular localization via immune-gold electron-microscopy showed that β- amylase was predominantly located together with starch granules, while the gold particles were scarcely found in other sub-cellular compartments. The result suggested that this enzyme is compartmented together with its functional substrate supporting its function in catalyzing starch breakdown in living plant cells.
Abstract The ultra-structure of mother and outer daughter scales of Lilium Oriental hybrid Sorbonne were studied using transmission electron microscope to examine the sub-cellular localization of starch and lipid droplets during growth and development from shoot emergence to senescence. The contents of starch granules and lipid droplets in the cell of the mother scales decreased significantly from shoot emergence to anthesis, indicating that these scales served as a source for growth and development. After flowering, the number of starch granules and lipid droplets increased dramatically, and finally the cells were filled with the above molecules indicating that the bulb becomes a major sink during bulb enlargement. Ultrastructure observation also showed that symplastic pathway is the main pathway in cells in the exchange and transportation of material during bulb development. The activity of β-amylase, one of the key enzymes catalyzing starch breakdown, showed a similar trend. The enzyme sub-cellular localization via immune-gold electron-microscopy showed that β- amylase was predominantly located together with starch granules, while the gold particles were scarcely found in other sub-cellular compartments. The result suggested that this enzyme is compartmented together with its functional substrate supporting its function in catalyzing starch breakdown in living plant cells.
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Received: 29 March 2011
Accepted: 11 April 2012
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| Fund: This work was supported by the National Natural Science Foundation of China (31071815) and the National High- Tech R&D Program of China (2011AA100208). |
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Corresponding Authors:
Correspondence LV Ying-min, Mobile: 13240972239, Tel: +86-10-82376017, Fax:
+86-10-62336126, E-mail: luyingmin@bjfu.edu.cn
E-mail: luyingmin@bjfu.edu.cn
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| About author: WU Sha-sha, Mobile: 13260253902, E-mail: lanyou19842000@yahoo.com.cn |
Cite this article:
WU Sha-sha, WU Jin-di, JIAO Xue-hui, ZHANG Qi-xiang, , LV Ying-min.
2012.
The Dynamics of Changes in Starch and Lipid Droplets and Sub-Cellular Localization of β-Amylase During the Growth of Lily Bulbs. Journal of Integrative Agriculture, 12(4): 585-592.
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| [1]Chen J X, Ziv M. 2005. The effects of storage condition on starch metabolism and regeneration potentials of twinscales and inflorescence stem explants of Narcissus tazetta. In vitro Cellular and Developmental Biology-Plant, 41, 816-821. [2]Chen S. 1986. Carbohydrate metabolism in the narcissus leaf. Journal of Experimental Botany, 20, 302-316. [3]Gao W Y, Li Z L, Xiao P G. 1994. The anatomical study of Fritillaria thunbergii in the senescent process. Guihaia, 1, 65-69. [4]Kamenetsky R, Zemah H, Ranwala A P, Vergeldt F, Ranwala N K, Miller W B, van As H, Bendel P. 2003. Water status and carbohydrate pools in tulip bulbs during dormancy release. New Phytologist, 158, 109-118. [5]Kram A M, Oostergetel G T, Bruggen E F. 1993. Localization of branching enzyme in potato tuber cell with the use of immunoelectron microscopy. Plant Physiology, 101, 237-243. [6]Langens-Gerrits M M, Kuijpers A M, de Klerk G J, Croes AF. 2003a. Contribution of explants carbohydrate reserves and sucrose in the medium to bulb growth of lily regenerated on scale segments in vitro. Physiologial Plantarum, 117, 245-255. [7]Langens-Gerrits M M, Miller W B M, Croes A F, de Klerk G J. 2003b. Effect of low temperature on dormancy breaking and growth after planting in lily bulblets regenerated in vitro. Plant Growth Regulation, 40, 267-275. [8]Lizzotte P A, Henson C A, Duke S H. 1990. Purification and characterization of pea epicotyls ?-amylase. Plant Physiology, 92, 619-528. [9]Marinangeli P, Curvetto N. 1997. Bulb quality and traumatic acid influence bulblet formation scaling in Lilium species and hybrids. HortScience, 32, 584-606. [10]Miller G L. 1959. Use of dinitrosalicylic acid reagent for the determination of reducing sugars. Analytical Chemistry, 31, 426-428. [11]Miller W B, Langhans R W. 1990. Low temperature alters carbohydrate metabolism in easter lily bulbs. HortScience, 25, 463-465. [12]Monroe J D, Salminen M D, Preiss J. 1991. Nucleotide sequence of a cDNA clone encoding a ?-amylase from Arabidopsis thaliana. Plant Physiology, 97, 1599-1601. [13]Qin Y, Wang Y, Duan C Q, Zhang D P. 2003. ?-Amylase is predominantly localized to plastids in the developing tuberous root of sweet potato. Acta Botanica Sinica, 5, 581-588. [14]Roh S M, Wilkins H F. 1976. The physiology of dormancy and maturity of Lilium longiflorum Thnunb. cv. Nellie White bulb. I. Scale filling and seasonal changes in the nutrient elements. Journal of Korean Society for Horticultural Science, 2, 173-179. [15]Shin K S, Chakrabarty D, Peak K Y. 2002. Sprouting rate, change of carbohydrate contents and related enzymes during cold treatment of lily bulblets regenerated in vitro. Scientia Horticulturae, 96, 195-204. [16]Sun Z, Henson C A. 1991. A quantitative assessment of the importance of barley seed ?-amylase, ?-amylase, debranching enzyme, and ?-glucosidase in starch degradation. Archives of Biochemistry and Biophysics, 284, 298-305. [17]Taiz L. 1984. Plant cell expension. Annual Review of Plant Physiology and Plant Molecular Biology, 35, 585-657. [18]Turgeon R. 1989. The sink-source transition in leaves. Annual Review of Plant Physiology and Plant Molecular Biology, 40, 119-138. [19]Wang D Y, Lian Y, Zhu D W. 2008. Acid phosphatase activity may affect the tuber swelling by partially regulating sucrose-mediated sugar resorption in potato. Journal of Integrative Plant Biology, 6, 733-741. [20]Wang Y Z, Zhang D P. 2002. ?-Amylase in developing apple fruit: activities, amounts and subcellular localization. Science in China (Series C), 3, 201-210. [21]Wu L H, Liu J L. 1994. Chemical and physical characters of starch granule of lily bulb. Shandong Food Ferment, 4, 51-56. [22]Zhang Y, Sun H M, Shen X Q, Chen W Z. 2007. Changes in ultra-structure of starch granule in the middle scales of lilium davidii var. unicolor during the bulb development and storage at low temperature. Acta Botanica Sinica, 3, 699-704. |
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