Genetic diversity and elite gene introgression reveal the japonica rice breeding in northern China

doi:10.1016/S2095-3119(14)60898-4

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (5): 811-822.DOI: 10.1016/S2095-3119(14)60898-4

Genetic diversity and elite gene introgression reveal the japonica rice breeding in northern China

LIU Dan, WANG Jia-yu, WANG Xiao-xue, YANG Xian-li, SUN Jian, CHEN Wen-fu

1、Key Laboratory of Northeast Rice Biology and Breeding, Ministry of Agriculture/Key Laboratory of Northern japonica Super Rice Genetics and Breeding, Ministry of Education/Rice Research Institute, Shenyang Agricultural University, Shenyang 110866,
P.R.China
2、Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157041, P.R.China

收稿日期:2014-05-09 出版日期:2015-05-01 发布日期:2015-05-13
通讯作者: CHEN Wen-fu,Tel/Fax: +86-24-88487186, E-mail: wfchen5512@126.com
作者简介:LIU Dan, E-mail: danliu86@hotmail.com; WANG Jia-yu,E-mail: ricewjy@126.com;
基金资助:
This work was supported by the National Natural Science Foundation of China (31371586) and the Program for Liaoning Excellent Talents in University (LNET), China (LJQ2013075).

Genetic diversity and elite gene introgression reveal the japonica rice breeding in northern China

LIU Dan, WANG Jia-yu, WANG Xiao-xue, YANG Xian-li, SUN Jian, CHEN Wen-fu

1、Key Laboratory of Northeast Rice Biology and Breeding, Ministry of Agriculture/Key Laboratory of Northern japonica Super Rice Genetics and Breeding, Ministry of Education/Rice Research Institute, Shenyang Agricultural University, Shenyang 110866,
P.R.China
2、Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157041, P.R.China

Received:2014-05-09 Online:2015-05-01 Published:2015-05-13
Contact: CHEN Wen-fu,Tel/Fax: +86-24-88487186, E-mail: wfchen5512@126.com
About author:LIU Dan, E-mail: danliu86@hotmail.com; WANG Jia-yu,E-mail: ricewjy@126.com;
Supported by:
This work was supported by the National Natural Science Foundation of China (31371586) and the Program for Liaoning Excellent Talents in University (LNET), China (LJQ2013075).

摘要/Abstract

摘要： Abundant genetic diversity and rational population structure of germplasm benefit crop breeding greatly. To investigate genetic variation among geographically diverse set of japonica germplasm, we analyzed 233 japonica rice cultivars collected from Liaoning, Jilin and Heilongjiang provinces of China, which were released from 1970 to 2011 by using 62 simple sequence repeat (SSR) markers and 8 functional gene tags related to yield. A total of 195 alleles (Na) were detected with an average of 3.61 per locus, indicating a low level of genetic diversity level among all individuals. The genetic diversity of the cultivars from Jilin Province was the highest among the three geographic distribution zones. Moreover, the genetic diversity was increased slightly with the released period of cultivars from 1970 to 2011. The analysis of molecular variance (AMOVA) revealed that genetic differentiation was more diverse within the populations than that among the populations. The neighbor-joining (NJ) tree indicated that cultivar clusters based on geographic distribution represented three independent groups, among which the cluster of cultivars from Heilongjiang is distinctly different to the cluster of cultivars from Liaoning. For the examined functional genes, two or three allelic variations for each were detected, except for IPA1 and GW2, and most of elite genes had been introgressed in modern japonica rice varieties. These results provide a valuable evaluation for genetic backgrounds of current japonica rice and will be used directly for japonica rice breeding in future.

关键词: japonica rice cultivars , genetic diversity , elite gene introgression , simple sequence repeat , functional gene tags

Abstract: Abundant genetic diversity and rational population structure of germplasm benefit crop breeding greatly. To investigate genetic variation among geographically diverse set of japonica germplasm, we analyzed 233 japonica rice cultivars collected from Liaoning, Jilin and Heilongjiang provinces of China, which were released from 1970 to 2011 by using 62 simple sequence repeat (SSR) markers and 8 functional gene tags related to yield. A total of 195 alleles (Na) were detected with an average of 3.61 per locus, indicating a low level of genetic diversity level among all individuals. The genetic diversity of the cultivars from Jilin Province was the highest among the three geographic distribution zones. Moreover, the genetic diversity was increased slightly with the released period of cultivars from 1970 to 2011. The analysis of molecular variance (AMOVA) revealed that genetic differentiation was more diverse within the populations than that among the populations. The neighbor-joining (NJ) tree indicated that cultivar clusters based on geographic distribution represented three independent groups, among which the cluster of cultivars from Heilongjiang is distinctly different to the cluster of cultivars from Liaoning. For the examined functional genes, two or three allelic variations for each were detected, except for IPA1 and GW2, and most of elite genes had been introgressed in modern japonica rice varieties. These results provide a valuable evaluation for genetic backgrounds of current japonica rice and will be used directly for japonica rice breeding in future.

Key words: japonica rice cultivars , genetic diversity , elite gene introgression , simple sequence repeat , functional gene tags

LIU Dan, WANG Jia-yu, WANG Xiao-xue, YANG Xian-li, SUN Jian, CHEN Wen-fu. Genetic diversity and elite gene introgression reveal the japonica rice breeding in northern China[J]. Journal of Integrative Agriculture, 2015, 14(5): 811-822.

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Genetic diversity and elite gene introgression reveal the japonica rice breeding in northern China

Genetic diversity and elite gene introgression reveal the japonica rice breeding in northern China

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