Genetic Relationships Among Chinese Maize OPVs Based on SSR Markers

doi:10.1016/S2095-3119(13)60341-X

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (7): 1130-1137.DOI: 10.1016/S2095-3119(13)60341-X

Genetic Relationships Among Chinese Maize OPVs Based on SSR Markers

SONG Li-ya, LIU Xue, CHEN Wei-guo, HAO Zhuan-fang, BAI Li , ZHANG De-gui

1 Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100048,P.R.China
2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2012-10-14 出版日期:2013-07-01 发布日期:2013-07-04
通讯作者: Correspondence ZHANG De-gui, Tel: +86-10-82108596, Fax: +86-10-82108747, E-mail: zdgzdg2010@yahoo.com.cn
作者简介:SONG Li-ya, E-mail: songly200512@126.com; LIU Xue, E-mail: xliu@genetics.ac.cn;
基金资助:
The present study was supported by the National Natural Science Foundation of China (30571169) and the Earmarked Fund for Modern Agro-Industry Technology Research System of Maize, Ministry of Agriculture, China (2006-G3).

Genetic Relationships Among Chinese Maize OPVs Based on SSR Markers

SONG Li-ya, LIU Xue, CHEN Wei-guo, HAO Zhuan-fang, BAI Li , ZHANG De-gui

1 Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100048,P.R.China
2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2012-10-14 Online:2013-07-01 Published:2013-07-04
Contact: Correspondence ZHANG De-gui, Tel: +86-10-82108596, Fax: +86-10-82108747, E-mail: zdgzdg2010@yahoo.com.cn
About author:SONG Li-ya, E-mail: songly200512@126.com; LIU Xue, E-mail: xliu@genetics.ac.cn;
Supported by:
The present study was supported by the National Natural Science Foundation of China (30571169) and the Earmarked Fund for Modern Agro-Industry Technology Research System of Maize, Ministry of Agriculture, China (2006-G3).

摘要/Abstract

摘要： Bulk-SSR method was used to analyze the genetic diversity of 44 open-pollinated varieties collected from Henan, Shandong, Shanxi, and Jilin provinces and Guangxi Zhuang Autonomous Region, China using 70 pairs of SSR primers. The purposes of this study were to (1) compare the genetic diversity among 44 Chinese maize open-pollinated varieties; (2) estimate the minimum number of alleles for construction of a stable dendrogram; and (3) trace the genetic relationships among local germplasm from different regions of China. In total, these 70 SSR primers yielded 292 alleles in 176 samples (4×44) analyzed. The number of alleles per locus was 4.17 on average and ranged from 2 to 8. The highest number of alleles per open-pollinated variety (55.25) was detected in Shanxi germplasm, which indicated that open-pollinated varieties from Shanxi possessed the largest genetic diversity among those from the five locations. The correlation coefficients between different genetic similarity matrices suggested that 200 alleles were sufficient for analysis of the genetic diversity of these 44 open-pollinated varieties. The cluster analysis showed that 44 open-pollinated varieties collected from three growing regions in China were accurately classified into three groups that were highly consistent with their geographic origins, and there is no correlation between GS and geographic distance in this study.

关键词: Bulk-SSR , genetic diversity , maize , OPVs

Abstract: Bulk-SSR method was used to analyze the genetic diversity of 44 open-pollinated varieties collected from Henan, Shandong, Shanxi, and Jilin provinces and Guangxi Zhuang Autonomous Region, China using 70 pairs of SSR primers. The purposes of this study were to (1) compare the genetic diversity among 44 Chinese maize open-pollinated varieties; (2) estimate the minimum number of alleles for construction of a stable dendrogram; and (3) trace the genetic relationships among local germplasm from different regions of China. In total, these 70 SSR primers yielded 292 alleles in 176 samples (4×44) analyzed. The number of alleles per locus was 4.17 on average and ranged from 2 to 8. The highest number of alleles per open-pollinated variety (55.25) was detected in Shanxi germplasm, which indicated that open-pollinated varieties from Shanxi possessed the largest genetic diversity among those from the five locations. The correlation coefficients between different genetic similarity matrices suggested that 200 alleles were sufficient for analysis of the genetic diversity of these 44 open-pollinated varieties. The cluster analysis showed that 44 open-pollinated varieties collected from three growing regions in China were accurately classified into three groups that were highly consistent with their geographic origins, and there is no correlation between GS and geographic distance in this study.

Key words: Bulk-SSR , genetic diversity , maize , OPVs

SONG Li-ya, LIU Xue, CHEN Wei-guo, HAO Zhuan-fang, BAI Li , ZHANG De-gui. Genetic Relationships Among Chinese Maize OPVs Based on SSR Markers[J]. Journal of Integrative Agriculture, 2013, 12(7): 1130-1137.

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Genetic Relationships Among Chinese Maize OPVs Based on SSR Markers

Genetic Relationships Among Chinese Maize OPVs Based on SSR Markers

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