Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (5): 823-829.DOI: 10.1016/S2095-3119(14)60972-2

• 论文 • 上一篇    下一篇

Magnaporthe Rab5 homologs show distinct functions in nerve growth factor (NGF)-mediated neurite outgrowth and cell differentiation

 QI Yao-yao, LIANG Zhi-min, ZHOU Jie, WANG Zong-hua, LU Guo-dong, LI Guang-pu   

  1. 1、Key Laboratory of Biopesticides and Chemical Biology, Ministry of Education/Fujian Agriculture and Forestry University, Fuzhou
    350002, P.R.China
    2、Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma 73104, USA
  • 收稿日期:2014-12-18 出版日期:2015-05-01 发布日期:2015-05-13
  • 通讯作者: LU Guo-dong, Tel: +86-591-83789478,E-mail: gdlufafu@163.com; LI Guang-pu, Tel: +86-405-2712227,Fax: +86-405-2713910, E-mail: guangpu-li@ouhsc.edu
  • 作者简介:Received 18 December, 2014 Accepted 4 March, 2015 QI Yao-yao, Mobile: 15980545390, E-mail: wenxuerubing@sohu.com;
  • 基金资助:

    This work was supported, in whole or in part, by the National Institutes of Health Grant, USA (R01GM074692), the National Nature Sciences Foundation of China (31328002 and 31070124) and a scholarship from the China Scholarship Council.

Magnaporthe Rab5 homologs show distinct functions in nerve growth factor (NGF)-mediated neurite outgrowth and cell differentiation

 QI Yao-yao, LIANG Zhi-min, ZHOU Jie, WANG Zong-hua, LU Guo-dong, LI Guang-pu   

  1. 1、Key Laboratory of Biopesticides and Chemical Biology, Ministry of Education/Fujian Agriculture and Forestry University, Fuzhou
    350002, P.R.China
    2、Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma 73104, USA
  • Received:2014-12-18 Online:2015-05-01 Published:2015-05-13
  • Contact: LU Guo-dong, Tel: +86-591-83789478,E-mail: gdlufafu@163.com; LI Guang-pu, Tel: +86-405-2712227,Fax: +86-405-2713910, E-mail: guangpu-li@ouhsc.edu
  • About author:Received 18 December, 2014 Accepted 4 March, 2015 QI Yao-yao, Mobile: 15980545390, E-mail: wenxuerubing@sohu.com;
  • Supported by:

    This work was supported, in whole or in part, by the National Institutes of Health Grant, USA (R01GM074692), the National Nature Sciences Foundation of China (31328002 and 31070124) and a scholarship from the China Scholarship Council.

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摘要： Nerve growth factor (NGF) binds to TrkA and forms a NGF/TrkA complex at the cell surface, which is then internalized into signaling endosomes and promotes neuronal survival and neurite outgrowth. The small GTPase Rab5 is reported to localize on the plasma membrane and early endosomes, regulating endosome fusion. It was reported that endogenous Rab5 function may need to be suppressed during NGF-induced neurite outgrowth and cell differentiation. Two Rab5 homologs (MoRab5A: MGG_06241 and MoRab5B:MGG_01185) were characterized from the rice blast fungus Magnaporthe oryzae, and MoRab5B was identified as the Rab5 ortholog promoting early endosomal fusion, while MoRab5A specialized to perform a non-redundant function in endosomal sorting. In this study, we examined whether MoRab5A and MoRab5B play different roles in NGF-induced neurite outgrowth and cell differentiation in PC12 cells (a rat pheochromocytoma cell line). Our data showed that MoRab5B is a negative regulator of NGF signaling and neurite outgrowth in PC12 cells, similar to human Rab5 (hRab5). MoRab5B:WT inhibits NGF signaling-dependent neurite outgrowth while the dominant-negative MoRab5B mutant (MoRab5B:DN) enhances NGF signaling and neurite outgrowth. In contrast, MoRab5A:WT and MoRab5A:DN both significantly promote NGF-induced neurite outgrowth, indicating that MoRab5B is more similar to hRab5 than MoRab5A in the regulation of NGF signal transduction.

关键词: NGF , Rab5 GTPase , Magnaporthe oryzae , neurite ourgrowth and cell differentiation

Abstract: Nerve growth factor (NGF) binds to TrkA and forms a NGF/TrkA complex at the cell surface, which is then internalized into signaling endosomes and promotes neuronal survival and neurite outgrowth. The small GTPase Rab5 is reported to localize on the plasma membrane and early endosomes, regulating endosome fusion. It was reported that endogenous Rab5 function may need to be suppressed during NGF-induced neurite outgrowth and cell differentiation. Two Rab5 homologs (MoRab5A: MGG_06241 and MoRab5B:MGG_01185) were characterized from the rice blast fungus Magnaporthe oryzae, and MoRab5B was identified as the Rab5 ortholog promoting early endosomal fusion, while MoRab5A specialized to perform a non-redundant function in endosomal sorting. In this study, we examined whether MoRab5A and MoRab5B play different roles in NGF-induced neurite outgrowth and cell differentiation in PC12 cells (a rat pheochromocytoma cell line). Our data showed that MoRab5B is a negative regulator of NGF signaling and neurite outgrowth in PC12 cells, similar to human Rab5 (hRab5). MoRab5B:WT inhibits NGF signaling-dependent neurite outgrowth while the dominant-negative MoRab5B mutant (MoRab5B:DN) enhances NGF signaling and neurite outgrowth. In contrast, MoRab5A:WT and MoRab5A:DN both significantly promote NGF-induced neurite outgrowth, indicating that MoRab5B is more similar to hRab5 than MoRab5A in the regulation of NGF signal transduction.

Key words: NGF , Rab5 GTPase , Magnaporthe oryzae , neurite ourgrowth and cell differentiation