AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China

doi:10.1016/S1671-2927(00)8674

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (9): 1424-1433.DOI: 10.1016/S1671-2927(00)8674

AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China

LIU De-gao, ZHAO Ning, ZHAI Hong, YU Xiao-xia, JIE Qin, WANG Lian-jun, HE Shao-zhen, LIU Qing-chang

Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/China Agricultural University, Beijing 100193, P.R.China

收稿日期:2011-03-04 出版日期:2012-09-01 发布日期:2012-09-09
通讯作者: Correspondence LIU Qing-chang, Tel/Fax: +86-10-62733710, E-mail: liuqc@cau.edu.cn
基金资助:
This work was supported by China Agriculture Research System (Sweetpotato) and Chinese Universities Scientific Fund (2012YJ008).

AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China

LIU De-gao, ZHAO Ning, ZHAI Hong, YU Xiao-xia, JIE Qin, WANG Lian-jun, HE Shao-zhen, LIU Qing-chang

Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/China Agricultural University, Beijing 100193, P.R.China

Received:2011-03-04 Online:2012-09-01 Published:2012-09-09
Contact: Correspondence LIU Qing-chang, Tel/Fax: +86-10-62733710, E-mail: liuqc@cau.edu.cn
Supported by:
This work was supported by China Agriculture Research System (Sweetpotato) and Chinese Universities Scientific Fund (2012YJ008).

摘要/Abstract

摘要： AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta/Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance (AMOVA) revealed greater variations within regions (94.08%) than among regions (5.92%). Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between “Northern” and “Southern” sweetpotato varieties when Yangtze River was used as the dividing line.

关键词: AFLP fingerprinting, genetic diversity, main variety, sweetpotato (Ipomoea batatas (L.) Lam.)

Abstract: AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta/Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance (AMOVA) revealed greater variations within regions (94.08%) than among regions (5.92%). Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between “Northern” and “Southern” sweetpotato varieties when Yangtze River was used as the dividing line.

Key words: AFLP fingerprinting, genetic diversity, main variety, sweetpotato (Ipomoea batatas (L.) Lam.)

LIU De-gao, ZHAO Ning, ZHAI Hong, YU Xiao-xia, JIE Qin, WANG Lian-jun, HE Shao-zhen, LIU Qing-chang. AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China[J]. Journal of Integrative Agriculture, 2012, 12(9): 1424-1433.

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AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China

AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China

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