Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110

doi:10.1016/S1671-2927(00)8571

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (4): 521-·527.DOI: 10.1016/S1671-2927(00)8571

• 论文 • 下一篇

Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110

REN Qiang, LIU Hui-juan, ZHANG Zeng-yan, FENG Jing, XU Shi-chang, PU Zong-jun , XIN Zhiyong

1.The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 .Crop Research Institute, Sichun Academy of Agricultural Sciences, Chengdu 610066, P.R.China

收稿日期:2011-02-17 出版日期:2012-04-01 发布日期:2012-04-11
通讯作者: Correspondence ZHANG Zeng-yan, Tel: +86-10-82108781, E-mail: zhangzy@mail.caas.net.cn
基金资助:
This study was funded by the 863 Program of China (2006AA100120).

Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110

REN Qiang, LIU Hui-juan, ZHANG Zeng-yan, FENG Jing, XU Shi-chang, PU Zong-jun , XIN Zhiyong

1.The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 .Crop Research Institute, Sichun Academy of Agricultural Sciences, Chengdu 610066, P.R.China

Received:2011-02-17 Online:2012-04-01 Published:2012-04-11
Contact: Correspondence ZHANG Zeng-yan, Tel: +86-10-82108781, E-mail: zhangzy@mail.caas.net.cn
Supported by:
This study was funded by the 863 Program of China (2006AA100120).

摘要/Abstract

摘要： Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.). To diversify stripe rust-resistant resources for wheat breeding programs, a CIMMYT synthetic wheat line CI110 was identified to be resistant to 28 isolates of Pst, including 6 Chinese prevalent races CYR28-CYR33. Genetic analysis indicated that a single dominant gene was responsible for the stripe rust resistance in CI110, temporarily designated YrC110. A molecular map, harboring YrC110 and 9 linked SSR markers, was constructed through simple sequence repeat (SSR), and bulked segregant analysis. These linked markers and YrC110 were assigned on the short arm of chromosome 1B using the Chinese Spring nullisomic-tetrasomic and ditelosomic stocks. Gene postulation based on seedling reaction patterns to 30 Pst isolates suggested that the resistance gene YrC110 seemed different from the other known resistance genes tested, such as Yr9, Yr10, Yr15, Yr24, and Yr26/YrCH42. Four SSR markers Xbarc187150, Xgwm18227, Xgwm11223, and Xbarc240292 distinguished YrC110 from Yr10, Yr15, Yr24, and Yr26/YrCH42, and could be used as diagnostic ones for YrC110 in wheat resistant breeding programs against stripe rust.

关键词: synthetic wheat, Puccinia striiformis f. sp. tritici, resistance gene, simple sequence repeat (SSR) marker, gene postulation

Abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.). To diversify stripe rust-resistant resources for wheat breeding programs, a CIMMYT synthetic wheat line CI110 was identified to be resistant to 28 isolates of Pst, including 6 Chinese prevalent races CYR28-CYR33. Genetic analysis indicated that a single dominant gene was responsible for the stripe rust resistance in CI110, temporarily designated YrC110. A molecular map, harboring YrC110 and 9 linked SSR markers, was constructed through simple sequence repeat (SSR), and bulked segregant analysis. These linked markers and YrC110 were assigned on the short arm of chromosome 1B using the Chinese Spring nullisomic-tetrasomic and ditelosomic stocks. Gene postulation based on seedling reaction patterns to 30 Pst isolates suggested that the resistance gene YrC110 seemed different from the other known resistance genes tested, such as Yr9, Yr10, Yr15, Yr24, and Yr26/YrCH42. Four SSR markers Xbarc187150, Xgwm18227, Xgwm11223, and Xbarc240292 distinguished YrC110 from Yr10, Yr15, Yr24, and Yr26/YrCH42, and could be used as diagnostic ones for YrC110 in wheat resistant breeding programs against stripe rust.

Key words: synthetic wheat, Puccinia striiformis f. sp. tritici, resistance gene, simple sequence repeat (SSR) marker, gene postulation

REN Qiang, LIU Hui-juan, ZHANG Zeng-yan, FENG Jing, XU Shi-chang, PU Zong-jun , XIN Zhiyong. Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110[J]. Journal of Integrative Agriculture, 2012, 12(4): 521-·527.

参考文献

[1]Chen X M. 2005. Epidemiology and control of stripe rust on wheat. Canadian Journal of Plant Pathology, 27, 314-337.

[2]Chen X M, Moore M, Milus E A, Long D, Marshall D, Line R F, Jackson L. 2002. Wheat stripe rust epidemics and races of Puccinia striiformis f. sp. tritici in the United States in 2000. Plant Disease, 86, 39-46.

[3]Chen X M, Moore M K, Wood D A. 2003. Epidemiology and control of stripe rusts of wheat and barley in the United States. In: 8th International Congress of Plant Pathology. vol. 2, 2-7 Feb 2003, Christchurch, New Zealand. p. 118.

[4]Cheng P, Chen X M. 2010. Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s. Theoretical and Applied Genetics, 121, 195-204.

[5]Flor H H. 1971. The current status of the gene for gene concept. Annual Review Phytopathology, 9, 275-296.

[6]Heun M, Fischbeck G. 1987. Identification of wheat powdery mildew resistance genes by analyzing host-pathogen interactions. Plant Breeding, 98, 124-129.

[7]Kosambi D D. 1944. The estimation of map distances from recombination values. Annnals Eugenics, 12, 172-175.

[8]Li G Q, Li Z F, Yang W Y, Zhang Y, He Z H, Xu S C, Singh R P, Qu Y Y, Xia X C. 2006. Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26. Theoretical and Applied of Genetics, 112, 1434-1440.

[9]Li Q, Chen X M, Wang M N, Jing J X. 2011. Yr45, a new wheat gene for stripe rust resistance on the long arm of chromosome 3D. Theoretical and Applied Genetics, 122, 189-197.

[10]Lincoln S, Daly M, Lander E. 1992. Constructing genetic maps with Mapmaker/EXP3.0. Whitehead Institute Technology Report. 3rd ed. Whitehead Institute, Cambridge. Line R F, Chen X M. 1995. Successes in breeding for and managing durable resistance to wheat rusts. Plant Disease, 79, 1254-1255.

[11]Ma H, Singh R P, Mujeeb-Kazi A. 1995. Resistance to stripe rust in Triticum turgidum, T. tauschii and their synthetic hexaploids. Euphytica, 82, 117-124.

[12]Ma H, Singh R P, Mujeeb-Kazi A. 1997. Resistance to stripe rust in durum wheats, A-genome diploids and their amphiploids. Euphytica, 94, 279-286.

[13]Ma J X, Zhou R H, Dong Y S, Wang L F, Wang X M, Jia J Z. 2001. Molecular mapping and detection of the stripe rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers. Euphytica, 120, 219-226.

[14]McIntosh R A, Devos K M, Dubcovsky J, Rogers W J, Morris C F, Appels R, Somers D J, Anderson O A. 2007. Catalogue of gene symbol for wheat: 2007 supplement. [ ]. http://wheat.pw.usda.gov/ggpages/awn/ 53/Textfiles/WGC.html

[15]McIntosh R A, Dubcovsky J, Rogers W J, Morris C, Appels R, Xia X C. 2009. Catalogue of gene symbols for wheat: 2009 supplement. [ ]. http://www.shigen.nig.ac. jp/wheat/komugi/genes/macgene/supplement2009.pdf

[16]McIntosh R A, Lagudah E S. 2000. Cytogenetic studies in wheat XVIII. Gene Yr24 for resistance to stripe rust. Plant Breed, 119, 81-83.

[17]McIntosh R A, Yamazaki Y, Devos K M, Dubcovsky J, Rogers W J, Appels R. 2003. Catalogue of gene symbols for wheat. In: Pogna N E, Romano N, Pogna E A, Galterio G, eds., Proceedings of 10th International Wheat Genetics Symposium. vol. 4. Instituto Sperimentale per la Cerealcoltura, Rome. p. 8.

[18]Michelmore R W, Paran I, Kesseli V R. 1991. Identification of markers closely linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the Unite States of America, 88, 9828-9832.

[19]Mujeeb-Kazi A, Rosas V, Roland S. 1996. Conservation of the genetic variation of Triticum tauschii (Coss.) Schmalh. (Aegilops squarrosa L.) in synthetic hexaploid wheats (T. turgidum L.×T. tauschii, 2n=6x=42, AABBDD) and its potential utilization for wheat improvement. Genetic Resource Crop Evolution, 43, 129-134.

[20]Mujeeb-Kazi A, Fuentes-Davila G, Villareal R L, Cortes A, Rosas V, Delgado R. 2001. Registration of 10 synthetic hexaploid wheat and six bread wheat germplasms resistant to Karnal bunt. Crop Science, 41, 1652-1653.

[21]Multani D S, Daliwal H S, Singh P, Gill K S. 1988. Synthetic amphiploids of wheat as a source of resistance to Karnal bunt (Neovossia indica). Plant Breeding, 100, 122-125.

[22]Peng J H, Fahima T, Röder M S, Huang Q Y, Dahan A, Li Y C, Grama A, Nevo E. 2000. High-density molecular map of chromosome region harbouring stripe-rust resistance genes YrH52 and Yr15 derived from wild emmer wheat, Triticum dicoccoides. Genetica, 109, 199-210.

[23]Pretorius Z A, Boshoff W H P, Kema G H J. 1997. First report of Puccinia striiformis f. sp. tritici on wheat in South Africa. Plant Disease, 81, 424. Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal M W, 1998. A microsatellite map of wheat. Genetics, 149, 2007-2023.

[24]Sharp P G, Kreis M, Shewry P R, Gale M D. 1988. Location of ?-amylase sequence in wheat and its relatives. Theoretical and Applied Genetics, 75, 289-290.

[25]Singh R P, Mujeeb-Kazi A, Huerta-Espino J. 1998. Lr46: A gene conferring slow-rusting resistance to leaf rust in wheat. Phytopathology, 88, 890-894.

[26]Somers D J, Isaac P, Edwards K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109, 1105-1114.

[27]Wan A M, Zhao Z H, Chen X M, He Z H, Jin S L, Jia Q Z, Yao G,Yang J X, Wang B T, Li G B, et al. 2004. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Disease, 88, 896-904.

[28]Wang L F, Ma J X, Zhou R H, Wang X M, Jia J Z. 2002. Molecular tagging of the yellow rust resistance gene Yr10 in common wheat, P.I. 178383 (Triticum aestivum L.). Euphytica, 124, 71-73.

[29]Wang L M, Zhang Z Y, Liu H J, Xu S C, He M Z, Liu H X, Veisz O, Xin Z Y. 2009. Identification, gene postulation and molecular tagging of a stripe rust resistance gene in synthetic wheat CI 142. Cereal Research Communication, 37, 209-215.

[30]Wellings C R, Wright D G, Keiper F, Loughman R. 2003. First detection of wheat stripe rust in Western Australia: evidence for a foreign incursion. Australia Plant Pathology, 32, 321-322.

[31]Zhang P, McIntosh R A, Hoxha S, Dong C M. 2009. Wheat stripe rust resistance genes Yr5 and Yr7 are allelic. Theoretical and Applied Genetics, 120, 25-29.

[32]Zakari A, McIntosh R A, Hovmoller M S, Wellings C R, Shariflou M R, Hayden M, Bariana H S. 2003. Recombination of Yr15 and Yr24 in chromosome 1BS. In: Pogna N E, Romano N, Pogna E A, Alterio G, eds., Proceedings of 10th International Wheat Genetics Symposium. vol. 1. Instituto Sperimentale per la Cerealcoltura, Rome. pp. 417-420.

Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110

Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	HAN Liu-sha, LI Zai-feng, WANG Jia-zhen, SHI Ling-zhi, ZHU Lin, LI Xing, LIU Da-qun, Syed J A Shah. Molecular mapping of leaf rust resistance genes in the wheat line Yu 356-9[J]. Journal of Integrative Agriculture, 2015, 14(7): 1223-1228.
[2]	ZHANG Dong, OUYANG Shu-hong, WANG Li-li, CUI Yu, WU Qiu-hong, LIANG Yong, WANG Zhen-zhong, XIE Jing-zhong, ZHANG De-yun, WANG Yong, CHEN Yong-xing, LIU Zhi-yong. Comparative genetic mapping revealed powdery mildew resistance gene MlWE4 derived from wild emmer is located in same genomic region of Pm36 and Ml3D232 on chromosome 5BL[J]. Journal of Integrative Agriculture, 2015, 14(4): 603-609.
[3]	REN Yong, LI Sheng-rong, WEI Yu-ming, ZHOU Qiang, DU Xiao-ying, HE Yuan-jiang, ZHENG You-liang. Molecular mapping of a stripe rust resistance gene in Chinese wheat cultivar Mianmai 41[J]. Journal of Integrative Agriculture, 2015, 14(2): 295-304.
[4]	REN Xiao-li, LIU Tai-guo, LIU Bo, GAO Li, CHEN Wan-quan. Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars[J]. Journal of Integrative Agriculture, 2015, 14(10): 1992-2001.
[5]	ZHANG Kun, XU Jian-fei, DUAN Shao-guang, PANG Wan-fu, BIAN Chun-song, LIU Jie and JIN. NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans[J]. Journal of Integrative Agriculture, 2014, 13(8): 1662-1671.
[6]	YU Ma, CHEN Guo-yue, ZHANG Lian-quan, LIU Ya-xi, LIU Deng-cai, WANG Ji-rui, PU Zhien, ZHANG Li, LAN Xiu-jin, WEI Yu-ming, LIU Chun-ji , ZHENG You-liang. QTL Mapping for Important Agronomic Traits in Synthetic Hexaploid Wheat Derived from Aegiliops tauschii ssp. tauschii[J]. Journal of Integrative Agriculture, 2014, 13(8): 1835-1844.
[7]	LIU Ze-guang, YAO Wei-yuan, SHEN Xue-xue, CHAO Kai-xiang, FAN Yu, LI Min-zhou, WANG Baotong, LI Qiang , JING Jin-xue. Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D[J]. Journal of Integrative Agriculture, 2014, 13(12): 2577-2583.
[8]	Zheng gui-jie, Yang Yong-qing, Ma Ying, Yang Xiao-feng, Chen Shan-yu, Ren Rui, Wang Da-gang, Yang Zhong-lu , ZhI hai-jian. Fine Mapping and Candidate Gene Analysis of Resistance Gene RSC3Q to Soybean mosaic virus in Qihuang 1[J]. Journal of Integrative Agriculture, 2014, 13(12): 2608-2615.
[9]	SHI Li-hong, ZHANG Na, HU Ya-ya, WEI Xue-jun, YANG Wen-xiang , LIU Da-qun. Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars[J]. Journal of Integrative Agriculture, 2013, 12(9): 1580-1588.
[10]	MA Dong-fang, JING Jin-xue, HOU Dong-yuan, LI Qiang, ZHOU Xin-li, DU Jiu-yuan , LU Qing-lin. Genetics and Molecular Mapping of a High-Temperature Resistance Gene to Stripe Rust in Seeding-Stage in Winter Wheat Cultivar Lantian[J]. Journal of Integrative Agriculture, 2013, 12(6): 1018-1025.
[11]	MA Dong-fang, HOU Lu, TANG Ming-shuang, WANG Hai-ge, LI Qiang , JING Jin-xue. Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1[J]. Journal of Integrative Agriculture, 2013, 12(4): 638-645.
[12]	CHANG Jin-hua, CUI Jiang-hui, XUE Wei, ZHANG Qing-wen. Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers[J]. Journal of Integrative Agriculture, 2012, 12(7): 1086-1092.
[13]	ZHANG Nan, WANG Shen, WANG Hai-yan, LIU Da-qun. Isolation and Characterization of NBS-LRR Class Resistance Homologous Gene from Wheat[J]. Journal of Integrative Agriculture, 2011, 10(8): 1151-1158.
[14]	LI Ming-ju, FENG Jing, CAO Shi-qin, LIN Rui-ming, CHENG Geng, YU Ya-xiong, CHEN Wan-quan , XU Shi-chang . Postulation of Seedlings Resistance Genes to Yellow Rust in Commercial Wheat Cultivars from Yunnan Province in China [J]. Journal of Integrative Agriculture, 2011, 10(11): 1723-1731.
[15]	WU Xiao-ling, ZHANG Bao-qiang, SUN Shi, ZHAO Jin-ming, YANG Feng, GUO Na, GAI Jun-yi, XING Han. Identification, Genetic Analysis and Mapping of Resistance to Phytophthora sojae of Pm28 in Soybean[J]. Journal of Integrative Agriculture, 2011, 10(10): 1506-1511.