Phylogenetic Analysis of Citrus tristeza virus Isolates of Wild Type Citrus in China

doi:10.1016/S2095-3119(13)60730-3

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (12): 2669-2677.DOI: 10.1016/S2095-3119(13)60730-3

Phylogenetic Analysis of Citrus tristeza virus Isolates of Wild Type Citrus in China

YI Long, ZHOU Chang-yong

1、Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, P.R.China
2、National Navel Orange Engineering Technology Research Center, Ganzhou 341000, P.R.China

收稿日期:2013-10-22 出版日期:2014-12-01 发布日期:2014-12-10
通讯作者: ZHOU Chang-yong, Tel: +86-23-68349037, Fax: +86-23-68349592, E-mail: zhoucy@ cric.cn
作者简介:YI Long, Tel: +86-797-8393068, E-mail: yilong@gnnu.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (30900977), the Program for Changjiang Scholars and Innovative Research Team in Universities, China (PCSIRT, IRT0976), the Key Project 210111 of Ministry of Education of China, and the Young Scientist Cultivation Program of Jiangxi, China (2010DQ02300).

Phylogenetic Analysis of Citrus tristeza virus Isolates of Wild Type Citrus in China

YI Long, ZHOU Chang-yong

1、Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, P.R.China
2、National Navel Orange Engineering Technology Research Center, Ganzhou 341000, P.R.China

Received:2013-10-22 Online:2014-12-01 Published:2014-12-10
Contact: ZHOU Chang-yong, Tel: +86-23-68349037, Fax: +86-23-68349592, E-mail: zhoucy@ cric.cn
About author:YI Long, Tel: +86-797-8393068, E-mail: yilong@gnnu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (30900977), the Program for Changjiang Scholars and Innovative Research Team in Universities, China (PCSIRT, IRT0976), the Key Project 210111 of Ministry of Education of China, and the Young Scientist Cultivation Program of Jiangxi, China (2010DQ02300).

摘要/Abstract

摘要： The genetic variation and phylogenetic relationships of Citrus tristeza virus (CTV) isolates collected from Chinese wild type citrus were analyzed by comparing the sequences of nine genomic regions (p23, p20, p13, p18, p25, p27, POL, HEL and k17) with the CTV isolates of cultivated citrus from different countries. The results showed that the divergence pattern of genomic RNA of the CTV isolates from wild type citrus was similar to that of other isolates from cultivated citrus, the 3´ proximal region was relatively conserved, and the 5´ proximal region had greater variability. The nine genomic regions of CTV isolates analyzed were found to have been under purifying selection in the evolution process. Phylogenetic analysis showed that the eleven Chinese wild CTV isolates were located at different clades and did not reflect their geographical origins, suggesting genetic diversity among the Chinese wild CTV populations. These results will aid in the understanding of molecular evolution of the Chinese CTV populations.

关键词: Citrus tristeza virus , wild type citrus , genetic diversity , phylogenetic analysis

Abstract: The genetic variation and phylogenetic relationships of Citrus tristeza virus (CTV) isolates collected from Chinese wild type citrus were analyzed by comparing the sequences of nine genomic regions (p23, p20, p13, p18, p25, p27, POL, HEL and k17) with the CTV isolates of cultivated citrus from different countries. The results showed that the divergence pattern of genomic RNA of the CTV isolates from wild type citrus was similar to that of other isolates from cultivated citrus, the 3´ proximal region was relatively conserved, and the 5´ proximal region had greater variability. The nine genomic regions of CTV isolates analyzed were found to have been under purifying selection in the evolution process. Phylogenetic analysis showed that the eleven Chinese wild CTV isolates were located at different clades and did not reflect their geographical origins, suggesting genetic diversity among the Chinese wild CTV populations. These results will aid in the understanding of molecular evolution of the Chinese CTV populations.

Key words: Citrus tristeza virus , wild type citrus , genetic diversity , phylogenetic analysis

YI Long, ZHOU Chang-yong. Phylogenetic Analysis of Citrus tristeza virus Isolates of Wild Type Citrus in China[J]. Journal of Integrative Agriculture, 2014, 13(12): 2669-2677.

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Phylogenetic Analysis of Citrus tristeza virus Isolates of Wild Type Citrus in China

Phylogenetic Analysis of Citrus tristeza virus Isolates of Wild Type Citrus in China

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