Pathotypes and Genetic Diversity of Chinese Collections of Elsinoë fawcettii Causing Citrus Scab

doi:10.1016/S2095-3119(13)60522-5

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (6): 1293-1302.DOI: 10.1016/S2095-3119(13)60522-5

Pathotypes and Genetic Diversity of Chinese Collections of Elsinoë fawcettii Causing Citrus Scab

HOU Xin, HUANG Feng, ZHANG Tian-yuan, XU Jian-guo, Hyde D Kevin , LI Hong-ye

1、Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2、Zhejiang Citrus Research Institute, Taizhou 318020, P.R.China
3、Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand

收稿日期:2013-04-08 出版日期:2014-06-01 发布日期:2014-06-06
通讯作者: LI Hong-ye, E-mail: hyli@zju.edu.cn
作者简介:HOU Xin, E-mail: houxin.sdau@163.com
基金资助:
This work was supported by the China Agriculture Research System (CARS-27) and the Technology Application Research Program for Public Interest of Zhejiang Province, China.

Pathotypes and Genetic Diversity of Chinese Collections of Elsinoë fawcettii Causing Citrus Scab

HOU Xin, HUANG Feng, ZHANG Tian-yuan, XU Jian-guo, Hyde D Kevin , LI Hong-ye

1、Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2、Zhejiang Citrus Research Institute, Taizhou 318020, P.R.China
3、Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand

Received:2013-04-08 Online:2014-06-01 Published:2014-06-06
Contact: LI Hong-ye, E-mail: hyli@zju.edu.cn
About author:HOU Xin, E-mail: houxin.sdau@163.com
Supported by:
This work was supported by the China Agriculture Research System (CARS-27) and the Technology Application Research Program for Public Interest of Zhejiang Province, China.

摘要/Abstract

摘要： Two scab diseases are currently recognized on citrus: citrus scab, caused by Elsinoë fawcettii, and sweet orange scab, caused by E. australis. Although these pathogens are economically important, there is no molecular data on these species in China. Here we use internal transcribed spacer sequence data to report on host-specificity and genetic relationships among 46 isolates collected from the main citrus varieties grown across China. All strains isolated were E. fawcettii. Based on pathogenicity testing on 9 different citrus species, isolates were divided into 11 pathotypes (SM, FBHR, SJCR, SPOJCR, SR, SOJG, SPOJC, SRGC, Lemon and two unnamed pathotypes). SM is a new pathotype, and two isolates did not fit into any of the known pathotypes of E. fawcettii. Inter-simple sequence repeat (ISSR-PCR) assays separated the E. fawcettii isolates into 10 subgroups; the groupings basically corresponded to the pathogenicity test.

关键词: Elsinoë, fawcettii , genetic diversity , ISSR , pathotypes

Abstract: Two scab diseases are currently recognized on citrus: citrus scab, caused by Elsinoë fawcettii, and sweet orange scab, caused by E. australis. Although these pathogens are economically important, there is no molecular data on these species in China. Here we use internal transcribed spacer sequence data to report on host-specificity and genetic relationships among 46 isolates collected from the main citrus varieties grown across China. All strains isolated were E. fawcettii. Based on pathogenicity testing on 9 different citrus species, isolates were divided into 11 pathotypes (SM, FBHR, SJCR, SPOJCR, SR, SOJG, SPOJC, SRGC, Lemon and two unnamed pathotypes). SM is a new pathotype, and two isolates did not fit into any of the known pathotypes of E. fawcettii. Inter-simple sequence repeat (ISSR-PCR) assays separated the E. fawcettii isolates into 10 subgroups; the groupings basically corresponded to the pathogenicity test.

Key words: Elsinoë, fawcettii , genetic diversity , ISSR , pathotypes

HOU Xin, HUANG Feng, ZHANG Tian-yuan, XU Jian-guo, Hyde D Kevin , LI Hong-ye. Pathotypes and Genetic Diversity of Chinese Collections of Elsinoë fawcettii Causing Citrus Scab[J]. Journal of Integrative Agriculture, 2014, 13(6): 1293-1302.

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Pathotypes and Genetic Diversity of Chinese Collections of Elsinoë fawcettii Causing Citrus Scab

Pathotypes and Genetic Diversity of Chinese Collections of Elsinoë fawcettii Causing Citrus Scab

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