Parasitism and pathogenicity of Radopholus similis to Ipomoea aquatica, Basella rubra and Cucurbita moschata and genetic diversity of different populations

doi:10.1016/S2095-3119(14)61003-0

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (1): 120-134.DOI: 10.1016/S2095-3119(14)61003-0

所属专题：线虫合辑Nematology

Parasitism and pathogenicity of Radopholus similis to Ipomoea aquatica, Basella rubra and Cucurbita moschata and genetic diversity of different populations

LI Yu, WANG Ke, XIE Hui, XU Chun-ling, WANG Dong-wei, LI Jing, HUANG Xin, PENG Xiao-fang

Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, South China Agricultural University, Guangzhou 510642, P.R.China

收稿日期:2014-11-18 出版日期:2016-01-08 发布日期:2016-01-11
通讯作者: XIE Hui, Tel: +86-20-38297286,E-mail: xiehui@scau.edu.cn
作者简介:LI Yu, Tel: +86-20-38297432, E-mail: limiao04@163.com;
基金资助:
This work was funded by the National Natural Science Foundation of China (31071665) and the Special Fund for Agro-Scientific Research in the Public Interest, China (200903040).

Parasitism and pathogenicity of Radopholus similis to Ipomoea aquatica, Basella rubra and Cucurbita moschata and genetic diversity of different populations

LI Yu, WANG Ke, XIE Hui, XU Chun-ling, WANG Dong-wei, LI Jing, HUANG Xin, PENG Xiao-fang

Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, South China Agricultural University, Guangzhou 510642, P.R.China

Received:2014-11-18 Online:2016-01-08 Published:2016-01-11
Contact: XIE Hui, Tel: +86-20-38297286,E-mail: xiehui@scau.edu.cn
About author:LI Yu, Tel: +86-20-38297432, E-mail: limiao04@163.com;
Supported by:
This work was funded by the National Natural Science Foundation of China (31071665) and the Special Fund for Agro-Scientific Research in the Public Interest, China (200903040).

摘要/Abstract

摘要： Ten populations of Radopholus similis from different ornamental hosts were tested for their parasitism and pathogenicity to water spinach (Ipomoea aquatic), malabar spinach (Basella rubra), and squash (Cucurbita moschata) in pots. The results showed all three plants were new hosts of R. similis. Growth parameters of plants inoculated with nematodes were significantly lower than those of healthy control plants. All R. similis populations were pathogenic to the three plants, but pathogenicity differed among populations from different hosts. The same R. similis populations also showed different pathogenic effects in the three different plants. RadN5 population from Anthurium andraeanum had the highest pathogenicity to the three studied plants. RadN1 from A. andraeanum had the lowest pathogenicity to squash and RadN7 from Chrysalidocarpus lutesens had the lowest pathogenicity to water spinach and malabar spinach. R. similis is usually associated with root tissues, but here we report that it could be found to move and feed in the stem bases of all three studied plants. Sequence and phylogenetic analyses of DNA markers of the 18S rRNA, 28S rRNA, ITS rRNA, and mitochondrial DNA gene sequences of ten R. similis populations revealed significant genetic diversity. RadN5 and RadN6 populations from anthurium showed a close genetic relationship and could be distinguished from other populations by PCR-RFLP. At the same time, RadN5 and RadN6 populations were the most pathogenic to three studied plants. These results confirm the existence of large biological variability and molecular diversity among R. similis populations from the same or different hosts, and these characteristics are related to pathogenic variability.

关键词: burrowing nematode , parasitism , pathogenicity , genetic diversity , PCR-RFLP

Abstract: Ten populations of Radopholus similis from different ornamental hosts were tested for their parasitism and pathogenicity to water spinach (Ipomoea aquatic), malabar spinach (Basella rubra), and squash (Cucurbita moschata) in pots. The results showed all three plants were new hosts of R. similis. Growth parameters of plants inoculated with nematodes were significantly lower than those of healthy control plants. All R. similis populations were pathogenic to the three plants, but pathogenicity differed among populations from different hosts. The same R. similis populations also showed different pathogenic effects in the three different plants. RadN5 population from Anthurium andraeanum had the highest pathogenicity to the three studied plants. RadN1 from A. andraeanum had the lowest pathogenicity to squash and RadN7 from Chrysalidocarpus lutesens had the lowest pathogenicity to water spinach and malabar spinach. R. similis is usually associated with root tissues, but here we report that it could be found to move and feed in the stem bases of all three studied plants. Sequence and phylogenetic analyses of DNA markers of the 18S rRNA, 28S rRNA, ITS rRNA, and mitochondrial DNA gene sequences of ten R. similis populations revealed significant genetic diversity. RadN5 and RadN6 populations from anthurium showed a close genetic relationship and could be distinguished from other populations by PCR-RFLP. At the same time, RadN5 and RadN6 populations were the most pathogenic to three studied plants. These results confirm the existence of large biological variability and molecular diversity among R. similis populations from the same or different hosts, and these characteristics are related to pathogenic variability.

Key words: burrowing nematode , parasitism , pathogenicity , genetic diversity , PCR-RFLP

LI Yu, WANG Ke, XIE Hui, XU Chun-ling, WANG Dong-wei, LI Jing, HUANG Xin, PENG Xiao-fang. Parasitism and pathogenicity of Radopholus similis to Ipomoea aquatica, Basella rubra and Cucurbita moschata and genetic diversity of different populations[J]. Journal of Integrative Agriculture, 2016, 15(1): 120-134.

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Parasitism and pathogenicity of Radopholus similis to Ipomoea aquatica, Basella rubra and Cucurbita moschata and genetic diversity of different populations

Parasitism and pathogenicity of Radopholus similis to Ipomoea aquatica, Basella rubra and Cucurbita moschata and genetic diversity of different populations

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