ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato (Ipomoea batatas L.) in China

doi:10.1016/S2095-3119(14)60779-6

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2346-2361.DOI: 10.1016/S2095-3119(14)60779-6

ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato (Ipomoea batatas L.) in China

ZHANG Kai, WU Zheng-dan, LI Yan-hua, ZHANG Han, WANG Liang-ping, ZHOU Quan-lu, TANG Dao-bin, FU Yu-fan, HE Feng-fa, JIANG Yu-chun, YANG Hang , WANG Ji-chun

1、College of Agronomy and Biotechnology, Southwest University, Beibei 400716, P.R.China
2、Sweet Potato Engineering and Technology Research Center, Beibei 400716, P.R.China
3、Engineering Research Center of South Upland Agriculture, Ministry of Education, Beibei 400716, P.R.China
4、College of Life Science, Southwest University, Beibei 400716, P.R.China
5、The Chongqing Three Gorges Academy of Agricultural Sciences, Wanzhou 404001, P.R.China
6、Nanchong Institute of Agricultural Sciences, Nanchong 637000, P.R.China

收稿日期:2012-09-12 出版日期:2014-11-06 发布日期:2014-11-14
通讯作者: WANG Ji-chun, Tel/Fax: +86-23-68250469, E-mail: wjchun@swu.edu.cn
作者简介:ZHANG Kai, Tel: +86-23-68251264, Fax: +86-23-68250469, E-mail: kaizhang2013@gmail.com; WU Zheng-dan, E-mail: wudandan0905@163.com;* These authors contributed equally to this study.
基金资助:
This work was supported by the National Natural Science Foundation of China (31101192), the Animal and Plant Breeding Project of Chongqing, China (cstc2010AB1053), the Application Development Key Project of Chongqing, China (cstc2013yykfb80010), the Fundamental Research Funds for the Central Universities, China (XDJK2011C004), and the “111” Project (B12006) of Ministry of Education, China.

ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato (Ipomoea batatas L.) in China

ZHANG Kai, WU Zheng-dan, LI Yan-hua, ZHANG Han, WANG Liang-ping, ZHOU Quan-lu, TANG Dao-bin, FU Yu-fan, HE Feng-fa, JIANG Yu-chun, YANG Hang , WANG Ji-chun

1、College of Agronomy and Biotechnology, Southwest University, Beibei 400716, P.R.China
2、Sweet Potato Engineering and Technology Research Center, Beibei 400716, P.R.China
3、Engineering Research Center of South Upland Agriculture, Ministry of Education, Beibei 400716, P.R.China
4、College of Life Science, Southwest University, Beibei 400716, P.R.China
5、The Chongqing Three Gorges Academy of Agricultural Sciences, Wanzhou 404001, P.R.China
6、Nanchong Institute of Agricultural Sciences, Nanchong 637000, P.R.China

Received:2012-09-12 Online:2014-11-06 Published:2014-11-14
Contact: WANG Ji-chun, Tel/Fax: +86-23-68250469, E-mail: wjchun@swu.edu.cn
About author:ZHANG Kai, Tel: +86-23-68251264, Fax: +86-23-68250469, E-mail: kaizhang2013@gmail.com; WU Zheng-dan, E-mail: wudandan0905@163.com;* These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (31101192), the Animal and Plant Breeding Project of Chongqing, China (cstc2010AB1053), the Application Development Key Project of Chongqing, China (cstc2013yykfb80010), the Fundamental Research Funds for the Central Universities, China (XDJK2011C004), and the “111” Project (B12006) of Ministry of Education, China.

摘要/Abstract

摘要： To determine the genetic diversity and population structure of sweet potato accessions cultivated in China, and to establish the genetic relationships among their germplasm types, a representative collection of 240 accessions was analyzed using inter-simple sequence repeat (ISSR) markers. The mean genetic similarity coefficient, Nei’s gene diversity, and shared allele distance of tested sweet potato accessions were 0.7302, 0.3167 and 0.2698, respectively. The 240 accessions could be divided into six subgroups and five subpopulations based on neighbor-joining (NJ) clustering and STRUCTURE results, and obvious genetic relationships among the tested sweet potato accessions were identified. The marker-based NJ clustering and population structure showed no distinct assignment pattern corresponding to flesh color or geographical ecotype of the tested sweet potato germplasm. Analysis of molecular variance (AMOVA) revealed small but significant difference between white and orange-fleshed sweet potato accessions. Small but significant difference were also observed among sweet potato accessions from the Southern summer-autumn sweet potato region, the Yellow River Basin spring and summer sweet potato region and the Yangtze River Basin summer sweet potato region. This study demonstrates that genetic diversity in the tested sweet potato germplasm collection in China is lower than that in some reported sweet potato germplasm collections from other regions. Pedigree investigations suggest that more diverse Chinese sweet potato varieties should be formed by broadening the selection scope of breeding parents and incorporating the introduced varieties into future breeding programs.

关键词: sweet potato , genetic diversity , population structure , agro-ecological zone , flesh color , ISSR

Abstract: To determine the genetic diversity and population structure of sweet potato accessions cultivated in China, and to establish the genetic relationships among their germplasm types, a representative collection of 240 accessions was analyzed using inter-simple sequence repeat (ISSR) markers. The mean genetic similarity coefficient, Nei’s gene diversity, and shared allele distance of tested sweet potato accessions were 0.7302, 0.3167 and 0.2698, respectively. The 240 accessions could be divided into six subgroups and five subpopulations based on neighbor-joining (NJ) clustering and STRUCTURE results, and obvious genetic relationships among the tested sweet potato accessions were identified. The marker-based NJ clustering and population structure showed no distinct assignment pattern corresponding to flesh color or geographical ecotype of the tested sweet potato germplasm. Analysis of molecular variance (AMOVA) revealed small but significant difference between white and orange-fleshed sweet potato accessions. Small but significant difference were also observed among sweet potato accessions from the Southern summer-autumn sweet potato region, the Yellow River Basin spring and summer sweet potato region and the Yangtze River Basin summer sweet potato region. This study demonstrates that genetic diversity in the tested sweet potato germplasm collection in China is lower than that in some reported sweet potato germplasm collections from other regions. Pedigree investigations suggest that more diverse Chinese sweet potato varieties should be formed by broadening the selection scope of breeding parents and incorporating the introduced varieties into future breeding programs.

Key words: sweet potato , genetic diversity , population structure , agro-ecological zone , flesh color , ISSR

ZHANG Kai, WU Zheng-dan, LI Yan-hua, ZHANG Han, WANG Liang-ping, ZHOU Quan-lu, TANG Dao-bin, FU Yu-fan, HE Feng-fa, JIANG Yu-chun, YANG Hang , WANG Ji-chun. ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato (Ipomoea batatas L.) in China[J]. Journal of Integrative Agriculture, 2014, 13(11): 2346-2361.

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ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato (Ipomoea batatas L.) in China

ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato (Ipomoea batatas L.) in China

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