A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea var. capitata L.)

doi:10.1016/S2095-3119(13)60277-4

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (4): 596-602.DOI: 10.1016/S2095-3119(13)60277-4

A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea var. capitata L.)

CHEN Chen, ZHUANG Mu, FANG Zhi-yuan, WANG Qing-biao, ZHANG Yang-yong, LIU Yu-mei

1.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Horticulture College of Qingdao Agricultural University, Qingdao 266109, P.R.China

收稿日期:2011-12-05 出版日期:2013-04-01 发布日期:2013-04-07
通讯作者: Correspondence ZHUANG Mu, Tel: +86-10-82108756, Fax: +86-10-62174123, E-mail: zhuangmu@caas.cn
基金资助:
This research was supported by the National Science and Technology Ministry of China (2008BADB1B02 and 2009BADB8B03), the Core Research Budget of the Non-profit Governmental Research Institution (ICS, CAAS) (1610032011011), the China Agriculture Research System (CARS-25), and the National High Technology Research and Development Program of China (863 Program, 2012AA100101).

A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea var. capitata L.)

CHEN Chen, ZHUANG Mu, FANG Zhi-yuan, WANG Qing-biao, ZHANG Yang-yong, LIU Yu-mei

1.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Horticulture College of Qingdao Agricultural University, Qingdao 266109, P.R.China

Received:2011-12-05 Online:2013-04-01 Published:2013-04-07
Contact: Correspondence ZHUANG Mu, Tel: +86-10-82108756, Fax: +86-10-62174123, E-mail: zhuangmu@caas.cn
Supported by:
This research was supported by the National Science and Technology Ministry of China (2008BADB1B02 and 2009BADB8B03), the Core Research Budget of the Non-profit Governmental Research Institution (ICS, CAAS) (1610032011011), the China Agriculture Research System (CARS-25), and the National High Technology Research and Development Program of China (863 Program, 2012AA100101).

摘要/Abstract

摘要： The dominant genic male sterility (DGMS) gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage (Brassica oleracea var. capitata L.), has been successfully applied in hybrid seed production of several cabbage cultivars in China. During the development of dominant male sterility lines in cabbage, the conventional identification of homozygous male-sterile plants (CDMs399-3/CDMs399-3) is a laborious and time-consuming process. For marker-assisted selection (MAS) of the gene CDMs399-3 transferred into key spring cabbage line 397, expressed sequence tag-simple sequence repeats (EST-SSR) and SSR technology were used to identify markers that were linked to CDMs399-3 based on method of bulked segregant analysis (BSA). By screening a set of 978 EST-SSRs and 395 SSRs, a marker BoE332 linked to the CDMs399-3 at a distance of 3.6 cM in the genetic background of cabbage line 397 were identified. 7 homozygous male-sterile plants in population P1170 with 20 plants were obtained finally via MAS of BoE332. Thus, BoE332 will greatly facilitate the transferring of the gene CDMs399-3 into the key spring cabbage line 397 and improve the application of DGMS in cabbage hybrid breeding.

关键词: cabbage (Brassica oleracea var. capitata L.) , dominant genic male sterility (DGMS) , expressed sequence tag-simple sequence repeats (EST-SSR) , bulked segregant analysis (BSA) , marker-assisted selection (MAS)

Abstract: The dominant genic male sterility (DGMS) gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage (Brassica oleracea var. capitata L.), has been successfully applied in hybrid seed production of several cabbage cultivars in China. During the development of dominant male sterility lines in cabbage, the conventional identification of homozygous male-sterile plants (CDMs399-3/CDMs399-3) is a laborious and time-consuming process. For marker-assisted selection (MAS) of the gene CDMs399-3 transferred into key spring cabbage line 397, expressed sequence tag-simple sequence repeats (EST-SSR) and SSR technology were used to identify markers that were linked to CDMs399-3 based on method of bulked segregant analysis (BSA). By screening a set of 978 EST-SSRs and 395 SSRs, a marker BoE332 linked to the CDMs399-3 at a distance of 3.6 cM in the genetic background of cabbage line 397 were identified. 7 homozygous male-sterile plants in population P1170 with 20 plants were obtained finally via MAS of BoE332. Thus, BoE332 will greatly facilitate the transferring of the gene CDMs399-3 into the key spring cabbage line 397 and improve the application of DGMS in cabbage hybrid breeding.

Key words: cabbage (Brassica oleracea var. capitata L.) , dominant genic male sterility (DGMS) , expressed sequence tag-simple sequence repeats (EST-SSR) , bulked segregant analysis (BSA) , marker-assisted selection (MAS)

CHEN Chen, ZHUANG Mu, FANG Zhi-yuan, WANG Qing-biao, ZHANG Yang-yong, LIU Yu-mei. A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea var. capitata L.)[J]. Journal of Integrative Agriculture, 2013, 12(4): 596-602.

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A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea var. capitata L.)

A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea var. capitata L.)

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