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Table of Content

    01 March 2011, Volume 44 Issue 5
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS

    cDNA Cloning, Structure Prediction and Expression Pattern Analysis of Antiquitin in Wheat (Triticum aestivum L.)

    Scientia Agricultura Sinica. 2011, 44(5):  859-866 . 
    Abstract ( 941 )   PDF (484KB) ( 963 )   Save
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    【Objective】 The cDNA of Antiquitin (TaATQ) was cloned from wheat (Triticum aestivum L.), the sequence and structure features of its encoded protein were analyzed,and the expression levels were evaluated. 【Method】 Based on EST assembling, the cDNA of TaATQ was cloned by RT-PCR. The structure features was analyzed by homology modeling. The expression patterns of the gene in different tissues, during seeds development and under different stress, were investigated using quantitative real-time PCR (qRT-PCR). 【Result】 The cDNA includes 1 530 bp complete CDS and encodes a 54 kD protein. TaATQ belongs to the 7th family of aldehyde dehydrogenase super family, it shows 91.7%, 78.1%, 58.1%, 58.3% and 58.9% identity with Antiquitin of rice, garden pea, nematode, human and mouse respectively. Functional TaATQ may form a tetramer in cell , and each monomer is composed of three typical domains, a NAD+-binding domain, a catalytic domain and an oligomerization domain. The expression levels of TaATQ in both leaf and root were increased significantly during the application of high salt. Under drought stress, the transcripts of TaATQ was elevated only in leaf. 【Conclusion】 TaATQ has typical structure features of ALDH protein family, it plays an important role during the process of stress response. TaATQ can be used as a target gene for crop genetic improvement.

    Cloning and Activity Analysis of the Promoter of Potato Protein Kinase Gene StPK1
    WU Tian,XIE Cong-hua
    Scientia Agricultura Sinica. 2011, 44(5):  867-873 . 
    Abstract ( 978 )   PDF (752KB) ( 943 )   Save
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    【Objective】 The objective of the experiment is to study the function of potato (Solanum tuberosum L.) protein kinase gene StPK1. 【Method】 The promoters of StPK1 were cloned by TAIL-PCR from leaves of three potato cultivars with distinct resistance types to late blight: quantitative, qualitative and susceptible. The promoter cloned from the qualitative resistance potato was fused to the GUS to construct the plant expression vector, and then the chimeric gene was transferred into Nicotiana benthamiana by Agrobacterium tumefaciens-mediated method. The transformed plants were treated with water, Phytophthora infestans and salicylic acid (SA), and the expression activity of the promoter was analyzed as well. 【Result】 The length of the promoters obtained are 922 bp, 929 bp, and 922 bp, respectively. The structure comparison indicated that all the promoters possess TATA-box, CAAT-box and the elements responsive to plant disease infection as well as those related to temporal and spatial expression. Their main differences were only observed in a little change of bases. And the results demonstrated that GUS was expressed in the leaves of transgenic tobacco treated with P. infestans and SA. 【Conclusion】 It showed that the promoter is active and pathogen- and SA-inducible.

    QTL Mapping of Six Yield Traits in Kenaf
    CHEN Mei-xia,QI Jian-min,FANG Ping-ping,LI Ai-qing,WEI Cheng-lin,TAO Ai-fen,XU Jian-tang,XIE Zeng-rong,LIN Pei-qing,LAN Tao,WU Jian-mei,CHEN Fu-cheng
    Scientia Agricultura Sinica. 2011, 44(5):  874-883 . 
    Abstract ( 827 )   PDF (529KB) ( 882 )   Save
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    【Objective】 The objective of this study was to identify QTL controlling partial yield traits in kenaf which could promote the basic scientific research of molecular marker-assisted selection. 【Method】In this investigation, a population with 162 F2:3 individuals from the cross between Alian kenaf (from Egypt) and Fuhong 992 (from Fujian Agriculture and Forestry University) with fine qualities, high yield and disease resistance was used and two location field tests were conducted. A randomized complete block design was used, to charactererize six yield traits: plant height, stem diameter, number of nodes, fresh bark weight per plant, dry bark weight per plant, 1000-seed weight. By using the data of field tests and MCIM method, six important yield traits were located and their genetic interactions were analyzed. 【Result】 Correlation analysis of field performance indicated significant positive correlation among all the traits except 1000-seed weight. As a result, two QTLs of plant height, two QTLs of stem diameter, two QTLs of number of nodes, one QTL of fresh bark weight per plant, and two QTLs of dry bark weight per plant, and two QTLs of 1000-seed weight were mapped in two places. 【Conclusion】 The eleven QTLs identified mainly clustered in linkage groups 6, 11, 14, 9, 13, 17 and 4, and all of these QTL distributed unevenly on chromosomes and tended to cluster together.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY
    A Study on Seed Quality Standard System of Crops in Domestic and Abroad
    ZHANG Wan-song,WANG Chun-ping,ZHANG Ai-min,GUO Xiang-mo,ZHANG Wei,ZHI Hai-jian,TIAN Bao-ming
    Scientia Agricultura Sinica. 2011, 44(5):  884-897 . 
    Abstract ( 801 )   PDF (313KB) ( 1174 )   Save
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    【Objective】China’s seed industry is confronted with severe challenges, particularly, the establishment of crop seed quality standards is a key issue of this kind of technology industry. The objective of this paper is to explore new four rank quality standards by comparing international seed quality standards with standards in China.【Method】According to the development of Chinese seed industry, views of China’s seed quality standard were constructed by comparing those standards in 122 important international organizations and developed countries with the current standards of 13 corresponding crops in China, and analyzing their similarities and differences. 【Result】 There are two differences: One is the system of seed ranks. Seed classifications of China’s current grain, cotton, oil seeds and potato tubers quality standards were diversified, including two ranks, three ranks and four ranks. But there just were four ranks in developed countries. The other is the factor index systems of seed quality. The factors index of seed quality of grain, cotton, and oil seeds in developed countries include pure seed, inert matter, total other crop seeds, other varieties, other species, weed seeds, noxious and (or) harmful weed seeds, moisture, germination, etc. The disease index should be highlighted in the potato tubers. There were four indexes including purity, clarity, moisture, germination in the grain, cotton, and oil seeds and purity, irregular of tubers, uniformity of tubers, meantime lacked necessary disease indexes in the potato tubers in China. Two basic principles of establishment of the system of seed quality standards are formed: firstly, the systems of seed ranks should be reasonable and the breeders’ seeds should serve as provenances for repeated reproduction and restricted generation reproduction to maintain excellent varieties; secondly, it is necessary to improve the ample degree of the system of factors index that not only reflects the requirements of China’s modern seeds production, but also integrates with the international advanced technology to ensure production of high-quality seeds and brand of commodity seeds. A new exploring scheme of seed quality standard system was suggested.【Conclusion】China’s main crops’ seed quality standards should be unified both seed ranks systems and factor index systems to establish the foundation of China’s domestic and foreign brands of commodity seeds.

    A Photo-Thermal Resources Based System for Greenhouse Climate Zonation and Energy Consumption Estimation in China
    YAO Yi-ping,SU Gao-li,LUO Wei-hong,DAI Jian-feng
    Scientia Agricultura Sinica. 2011, 44(5):  898-908 . 
    Abstract ( 687 )   PDF (378KB) ( 897 )   Save
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    【Objective】A photo-thermal resources based system for greenhouse climate zonation and energy consumption estimation was developed for dynamic climate zonation and identification of the distributions of photo-thermal resources and energy consumption for greenhouse crop production in China. 【Method】 First, 10 indices for greenhouse climate zonation were determined based on historical climate data set. Based on the values of the 10 indices, climate zonation was implemented by means of fuzzy C-Means (FCM) clustering method and a photo-thermal resources based system for dynamic climate zonation was developed. Secondly, a computer system was developed for climate zonation and energy consumption estimation for greenhouse crop production in China by integrating the photo-thermal resources based system for dynamic climate zonation with an energy balance based model for prediction greenhouse energy consumption. Thirdly, using 30 years (1971-2000) average daily weather data from 621 standard weather stations in China, a case study was conducted for greenhouse climate zonation and energy consumption estimation for a Venlo type greenhouse where infinite growth cucumber and tomato crops grow yearly around.【Result】The case study shows that China can be classified into 3 main regions (suitable, sub-suitable and unsuitable) and 9 sub-regions for greenhouse crop production. The suitable region is characterized with long period suitable for greenhouse crop production and low energy consumption for greenhouse heating. In this region, the major climate factor determining crop yield and profitability of greenhouse crop production is the total global ration during the period suitable for greenhouse crop production. The sub-suitable and unsuitable regions are characterized with long period and large energy consumption required for heating whereas low energy consumption for cooling in summer. In the later two regions, the major climate factor determining crop yield and profitability of greenhouse crop production is the accumulated negative temperature during the period for greenhouse heating. 【Conclusion】 The system developed in this study can be used not only for dynamic climate zonation and the identification of the distributions of photo-thermal resources for greenhouse crop production with historical climate data, but also for estimations of energy consumption for yearly around crop production in different types of greenhouse under different set-points for greenhouse temperature control. Therefore, the system has the potential to be used for assessing the risk for greenhouse investment and optimizing greenhouse structure and climate control from energy saving point of view.

    Effects of Alternative Selection of Maize Inbred Lines on Stress Tolerance Improvement
    MA Jun-feng,LIU Tian-xue,LI Chao-hai,YIN Shu-zhen
    Scientia Agricultura Sinica. 2011, 44(5):  909-916 . 
    Abstract ( 721 )   PDF (322KB) ( 625 )   Save
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    【Objective】 The effects of alternative selection on drought tolerance, disease resistance and yield in maize inbred lines were studied. 【Method】 Alternative selection on disease resistance, drought tolerance and high yield was carried out in a shuttle breeding program between Henan and Hainan Provinces by using the parents of the most popular elite maize hybrid Zhengdan 958, Zheng 58 and Chang 7-2. The gray leaf spot disease index, MDA content, drought tolerance coefficient and yield of maize inbred lines were determined in a field experiment and a pot experiment, respectively. 【Result】With the advance of alternative selection generations, compared with the original material, the gray leaf spot disease index of Zheng 58 in the first to fourth generations decreased by 16.1%, 16.6%, 31.5% and 33.1%, respectively, and that of Chang 7-2 in the first to fourth generations decreased by 28.1%, 29.7%, 45.3% and 49.5%, respectively, and gradually increased in drought tolerance coefficient and decreased in MDA content as advance of alternative selection generations. The average grain yields of Zheng 58 and Chang 7-2 in fourth generation increased by 8.3% and 9.1%, respectively.【Conclusion】The disease resistance, drought tolerance and yield increased in Zheng 58 and Chang 7-2 as four generations of alternative selection. The results from this study suggested that alternative selection is effective to improve maize inbred lines for stress resistance.

    Effect of Nitrogen, Phosphorus, Potassium, and Boron Fertilizers on Yield and Profit of Hybrid Rapeseed (Brassica napus L.)
    ZOU Xiao-yun,CHEN Lun-lin,LI Shu-yu,ZOU Xiao-fen,ZHANG Jian-mo,SONG Lai-qiang
    Scientia Agricultura Sinica. 2011, 44(5):  917-924 . 
    Abstract ( 1072 )   PDF (231KB) ( 804 )   Save
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    【Objective】 The objective of the research was to investigate the effect of nitrogen (N), phosphorus (P), potassium (K), and boron (B) on rapeseed yield, yield related traits, and profit of hybrid rapeseed (Brassica napus L.). 【Method】 Field experiments were conducted at 12 sites in Jiangxi provinces during 2008-2009. 【Result】 The combined application of NPKB increased the yield of rapeseed significantly. The average yield of NPKB treatment was 2 015 kg?hm-2. The average yield of CK treatment was 894 kg?hm-2, which was 1 121, 839, 758, 746, and 249 kg?hm-2 lower than those of NPKB, NPK , NKB, NPB, and PKB treatments, respectively. The average profit of NPKB treatment was up to 5 449.5 yuan/hm2 according to the prices of fertilizer and rapeseed during the production season. Compared with CK treatments,profit of NPKB, NPK, NKB, and NPB treatments increased by 2 231.1, 1 298.4, 1 280.7, and 1 541.1 yuan/hm2, respectively, while the profit of the treatment PKB decreased by 206.1 yuan/hm2. 【Conclusion】 It was concluded that the combination of NPKB fertilizers significantly increased both yield and profits of the hybrid rapeseed (Brassica napus L.). Utilization of nitrogen, phosphorus, potassium and boron fertilizers is the guarantee of realization of maximum hybrid rapeseed.

    PLANT PROTECTION
    Relationship Between DHN Melanin and Formation of Appressorium Turgor Pressure of Setosphaeria turcica
    CAO Zhi-yan,JIA Hui,ZHU Xian-ming,DONG Jin-gao
    Scientia Agricultura Sinica. 2011, 44(5):  925-932 . 
    Abstract ( 956 )   PDF (450KB) ( 771 )   Save
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    【Objective】 The objective of this study is to discuss the effect of intracellular DHN melanin of Setosphaeria turcica on the appressorium turgor pressure generated and elucidate the invasive mechanism of S. turcica. 【Method】 In this research, the best conditions for appressorium formation of S. turcica were determined by inducing appressorium production. The appressorium cell wall pore sizes and turgor pressures of wild-type isolate 01-23 and melanin-deficient strains △St3hnr of S. turcica were measured by a solute exclusion technique and incipient-cytorrhysis technique. 【Result】 The appressorium pore size of wild-type isolate 01-23 was 2.1-2.7 nm and it had a 5.4 MPa turgor pressure, while it was shown a range of 2.7-3.3 nm in melanin-deficient strains △St3hnr and a lower pressure about 4.1 MPa. The results indicated that appressorium without melanin could not form a high turgor pressure resulting in losing the ability for penetrating. 【Conclusion】 It is suggested that the melanin layer is significant in accumulation of excess turgor pressure and the turgor pressure of S. turcica play a major role in substance penetration.

    Cloning and Expression of the Gene Encoding the Protein Kinase C in Setosphaeria turcica
    ZHAO Wei,WANG Qian,HAO Zhi-min,WANG Qing,SONG Wen-jing,HAN Jian-min,DONG Jin-gao
    Scientia Agricultura Sinica. 2011, 44(5):  933-938 . 
    Abstract ( 791 )   PDF (494KB) ( 604 )   Save
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    【Objective】 The aim of this research was to clone the gene encoding the protein kinase C (PKC) and its promoter in Setosphaeria turcica, analyze the gene copy number in the genome of S. turcica and study the effects of different conditions on expression of PKC.【Method】 The homologous fragment of PKC was isolated by degenerate PCR, the 5′ and 3′ flanking sequences were cloned using genome walking, and the full length cDNA was obtained by RT-PCR. Gene structure and putative cis-acting elements were analyzed by bioinformatics methods. Then the gene’s expression was analyzed by semi-quantitative RT-PCR, when Setosphaeria turcica was cultured on different carbon/nitrogen media, and different abiological stresses.【Result】 PKC, encoding a protein of 1 171 amino acid residues, including 7 extons and 6 introns, was 3 837 bp of DNA and 3 516 bp of cDNA. There were CAAT-box and TATA-box in its upstream sequence, while the transcriptional initiator such as HSF, AP1 and Sp1-binding site, was observed. Southern blotting analysis indicated that there was a single copy of the PKC in the genome of S. turcica. By semi-quantitative RT-PCR analysis, it was found that the expression of PKC was the highest on saccharose as the carbon source, while it was distinctly inhibited by NH4+ and heavy metal ions such as Mn2+, Cu2+, and Zn2+. After addition of different concentrations of sorbitol, it has a positive correlation with the inhibition and concentrations. However, the highest expression was observed in response to isotonic NaCl. 【Conclusion】 Cloning of PKC and its promoter region in S. turcica have enriched the biological information resource of filamentous fungi, and thus laying a foundation for the functional analysis of the signal transduction pathway in phytopathogenic fungi.

    Roles of Pathogen-Originated H2O2 in the Non-Host Interaction Between Rice and Xanthomonas campestris pv. phaseoli
    LI Xin,PANG Xin-yue,ZHU Wen-xue,LI Xin-ling,LIU Yan,GUO Han,LI Hong-yu,ZHAO Chun-yan
    Scientia Agricultura Sinica. 2011, 44(5):  939-945 . 
    Abstract ( 988 )   PDF (605KB) ( 606 )   Save
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    【Objective】The objective of the paper is to study the roles of pathogen-originated hydrogen peroxide (H2O2) in the interaction between plant and pathogen.【Method】Localization of H2O2 accumulation was investigated in the non-host interaction between rice and Xanthomonas campestris pv. phaseoli (Xcp) by using histochemical analysis under electron microscopy. 【Result】The lack of Alkyl hydroperoxide reductase subunit C led to compensatory increase in the activities of H2O2 scavenging, and always resulted in significant decrease of endogenous H2O2 accumulation in ahpC mutant strain compared with the wild-type strain of Xcp, which has impact on the mean level of H2O2 accumulation in this interaction during the interaction with plant.【Conclusion】The results suggest that bacterial pathogen is a potential source for the H2O2 accumulation in the interaction between rice and Xcp. In addition, the H2O2 produced by bacteria may be an important cause for plant cell injury undergoing the HR during non-host resistance.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Effect of Different Irrigation and Fertilization Strategies on Nitrogen Balance and Soil Nitrate Movement of High-Yield Cotton System
    WANG Ping,CHEN Xin-ping,ZHANG Fu-suo,TIAN Chang-yan
    Scientia Agricultura Sinica. 2011, 44(5):  946-955 . 
    Abstract ( 934 )   PDF (301KB) ( 922 )   Save
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    【objective】 It is of great importance to study the effect of different water and nitrogen management strategies on the nitrogen balance and nitrate movement in soil profile. 【Method】 In a field experiment, the nitrogen balance and nitrate movement in soil profile of conventional irrigation + conventional N application, optimized irrigation + conventional N application, conventional irrigation + optimized N application, optimized irrigation + optimized N application were studied. 【Result】 The result showed that the optimized irrigation and fertilization strategy based on soil nitrate quick testing and soil moister measurement could dramatically reduce N losses from 163-294 kg?hm-2 to 19-87 kg?hm-2. Under conventional treatment, nitrate accumulation were greatly increasing in 60-200 cm soil profile after harvesting, and the ratio of nitrate accumulation amount to apparent N losses within 0.39-0.69. According to the results of nitrate movement process, nitrate accumulation become evident in soil profile of conventional N application, but not evident in optimized one. 【Conclusion】 Apparent N losses can be 52%-68% in conventional N application system, nitrate leaching out of root layer is one of important loss way. Optimized N application system in this experiment can dramatically reduce nitrate leaching.

    The Accumulation and Migration of Inorganic Phosphorus Fractions in Soils Under Greenhouse Cultivation
    YU Hai-ying,LI Ting-xuan,ZHANG Shu-jin,ZHANG Xi-zhou
    Scientia Agricultura Sinica. 2011, 44(5):  956-962 . 
    Abstract ( 717 )   PDF (342KB) ( 546 )   Save
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    【Objective】 The aims of this paper were to determine the reason for high input but low utilization of phosphorus (P) fertilizer, and to provide a basis for raising the utilization rate of P and reducing environmental pollution under greenhouse cultivation. 【Method】 To investigate the management of representative greenhouse cultivation in Shenyang, Liaoning Province, samples of soil from greenhouses of different planting years and also an open field within the researched area were collected and analyzed for the accumulation of inorganic P fractions. 【Result】 In greenhouse soil, the contents of total P, inorganic P and available P all increased, with their maximum values in the topsoil (0-20 cm), and average values of 3.1, 3.3 and 3.6 times that of the open field soil, respectively. The content of inorganic P accounted for 92.1% of total P, while available P only accounted for 16.6%. In greenhouse soil, the average contents of Ca8-P, Al-P, Ca2-P, Fe-P, O-P and Ca10-P were 10.2, 5.9, 5.0, 3.1, 2.7 and 1.5 times that of the open field soils, respectively, and the contents of each component of total P were Al-P (26.1%) > Fe-P (18.1%) > O-P (17.6%) > Ca10-P (14.4%) > Ca8-P (10.5%) > Ca2-P (5.6%). The nutrient availability of Ca2-P was high but its accumulation was low, while the availabilities of O-P and Ca10-P were high but their accumulations were low, which seriously decreased the utilization rate of P. In greenhouse soil, 49.3% of total inorganic P in topsoil (0–20 cm) was Al-P and Fe-P, while 46.6–78.2% of total inorganic P in deep soil was O-P and Ca10-P, which both increased with depth of soil. 【Conclusion】 Under greenhouse cultivation, the content of P increased greatly, with more than half of the total being Ca10-P, O-P and Fe-P. Improving the bioavailability of these components of inorganic P with low nutrient availability is the key to establish an environment-friendly nutrient management system compatible with greenhouse conditions.

    HORTICULTURE
    Effects of Doubled CO2 Concentration on Photosynthetic Characteristics of Cucumber Seedlings Under Drought Stresses
    LI Qing-ming,LIU Bin-bin,ZOU Zhi-rong
    Scientia Agricultura Sinica. 2011, 44(5):  963-971 . 
    Abstract ( 739 )   PDF (405KB) ( 830 )   Save
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    【Objective】 The purpose of this study is to explore the photosynthetic response and mechanism of cucumber seedlings to double CO2 concentration combined with drought stresses.【Method】 Using split plot design, the effects of doubled CO2 concentration on photosynthesis of cucumber (Cucumis sativus L. ‘Jinyou No.1’) seedlings under drought stresses were investigated. The main plot was two CO2 concentrations (ambient CO2 concentration, ≈380 μmol?mol-1, and doubled CO2 concentration, 760±20 μmol?mol-1), and the split-plot was three water treatments (control, moderate drought stress and severe drought stress) simulated by PEG 6000.【Result】 Drought stresses remarkably reduced net photosynthetic rate, transpiration rate and stomatal conductance of leaves of cucumber seedlings. Under the condition of doubled CO2 concentration, net photosynthetic rate dramatically increased while transpiration rate and stomatal conductance decreased, thereby water use efficiency dramatically increased. The interact effects of doubled CO2 concentration and drought stress on net photosynthetic rate, transpiration rate, water use efficiency as well as stomatal conductance were all significant (P<0.01). Drought stresses significantly reduced apparent quantum efficiency, maximum assimilation rate, maximum rate of carboxylation by Rubisco, PAR-saturated rate of electron transport, rate of triose phosphate utilization and light saturated points of leaves of cucumber seedlings, but enhanced light compensatory points. Under control and mild drought stress conditions, doubled CO2 concentration dramatically improved apparent quantum efficiency and maximum assimilation rate, but there were no significant differences under severe drought stress. Under control condition, doubled CO2 concentration dramatically enhanced maximum rate of carboxylation by Rubisco, PAR-saturated rate of electron transport and rate of triose phosphate utilization, but reduced CO2 compensatory points and respiration rate, there were no significant differences under drought stresses except CO2 compensatory points reduced by 4.9% under moderate drought stress. 【Conclusion】 The decrease of net photosynthesis under moderate drought stress was due to stoma restriction, while it was due to stoma and non-stoma combined restriction under severe drought stress. Drought stresses resulted in dramatic decline of photosynthetic electron transport and photophosphorylation capacity of cucumber seedlings. Doubled CO2 concentration improved water use efficiency of cucumber seedlings by improving net photosynthetic rate and reducing transpiration rate, and improved apparent quantum efficiency and CO2 assimilation maximum of cucumber seedlings under moderate drought stress condition to meliorate photosynthetic capacity. Therefore, it is speculated that in arid or semi-arid areas, CO2 fertilization in protected agriculture or rising atmospheric CO2 concentration in future could meliorate plant water status, enhance drought resistance ability and improve water use efficiency to some extent, subsequently alleviate the negative effect of drought stresses.

    Ananlysis of Transcriptome Differences Between tpa and Its Wild Type Flowers Based on the Microarray in Turnip
    WU Jian-feng,ZHANG Hai-juan,LU Hai-yu,WANG Fang-zhan,YU Xiao-lin
    Scientia Agricultura Sinica. 2011, 44(5):  972-981 . 
    Abstract ( 797 )   PDF (737KB) ( 816 )   Save
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    【Objective】 The possible reasons of the pistil abortion in turnip have been studied at the transcriptional level and it will be helpful to provide some useful clues to illuminate the molecular genetics mechanism of the gynoecium development in Brassica crops. 【Method】 The Arabidopsis ATH1 genome array was employed to analyze the transcriptome differences between the tpa mutants and their wild type flowers. Subsequently, the RT-PCR was used to confirm the genes with transcriptional difference. 【Result】 Data analysis results indicated that there were differences in 152 genes between the wild type (W1) and the pistil aborted completely (M3), likewise, 61 genes with transcriptional difference between the W1and the pistil aborted partially (I2), and 24 genes with transcriptional difference between the I2 and M3. All of the previous genes were classified into the following in functional categoryies: cell wall biosynthesis and regulation, defense and stressing reaction, transcriptional regulation, protein metabolism and normal metabolism, respectively. Out of the 41 genes, 9 genes with significant temporal and spatial transcription difference (At2g42840, At1g57750, At5g20630, At2g03090, At3g08030, At5g08000, At2g28790, At5g63310, At2g24270) were obtained through the RT-PCR screening and confirmation. 【Conclusion】 The Arabidopsis ATH1 genome array can be successfully utilized to analyze the transcriptome differences between the tpa mutants and their wild type. And the 9 genes with significant temporal and spatial transcription difference, are not only important to the reproductive process including gynoecium development, but also involve in the vegetative processes.

    Effect of Boron on Leaf Boron Forms of Newhall Navel Orange Grafted on Two Rootstocks
    LIU Gui-dong,JIANG Cun-cang,WANG Yun-hua,PENG Shu-ang,ZENG Qing-luan
    Scientia Agricultura Sinica. 2011, 44(5):  982-989 . 
    Abstract ( 991 )   PDF (274KB) ( 588 )   Save
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    【Objective】To clarify the influence of boron (B) treatments on utilization ability of B in Newhall navel orange(Citrus sinensis Osbeck ‘Newhall’)grafted on citrange [C. sinensis (L.) Osb.× Poncirus trifoliata (L.) Raf.] or trifoliate orange [Poncirus trifoliata(L.)Raf.] at cellular level.【Method】Nutrient solution culture method was used in this study. The difference of leaf B forms between the two rootstocks was analyzed according to the concentration of B forms and their relative relationship.【Result】The results showed that the free B concentration of citrange was relatively lower and the sime-bound B concentration was relatively higher in the treatments of B deficiency (B0, B 0 mg?L-1), normal B (B05, B 0.5 mg?L-1) and high B (B50, B 5.0 mg?L-1), on the contrary, in trifoliate orange. R value of citrange was 2.31, 1.23, and 1.06 in the treatments of B0, B05, B50,respectively, which was much higher than that (1.80, 0.57, 0.82) of trifoliate orange in the same treatment. The free B and semi-bound B concentrations of citrange (16.39,17.38 mg?kg-1) were more higher than that of trifoliate orange (8.60 and 7.02 mg?kg-1) in the treatment of B50. Under the conditions of normal or high B, the bound B concentration of citrange was 7.34 and 7.43 mg?kg-1, respectively, there was no significant difference, while the bound B concentration of trifoliate orange was the highest (9.20 mg?kg-1) under the condition of high boron.【Conclusion】B transmembrane transport was easier in citrange-grafted plants compared to that in trifoliate orange, so B utilization ability of Newhall navel orange grafted on citrange was higher. Furthermore, B could fully bind the cell wall in citrange-grafted plants at relatively low B supply.

    Expression of MhWRKY1 Gene Induced by the Elicitors SA,MeJA, ACC and the Apple Ring Spot Pathogen
    ZHANG Ji-yu,TONG Zhao-guo,GAO Zhi-hong ,LUO Chang-guo,QU Shen-chun,ZHANG Zhen
    Scientia Agricultura Sinica. 2011, 44(5):  990-999 . 
    Abstract ( 873 )   PDF (802KB) ( 593 )   Save
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    【Objective】This study aimed to clone a full length cDNA of MhWRKY1 from Malus hupehensis and analyze the expression pattern of MhWRKY1 in different tissues (leaf, stem and root) and the expression pattern after treatment with the elicitors salicylic acid (SA), methyl jasmonate (MeJA), 1-aminocyclopropane-1-carboxylic acid (ACC) and apple ring spot pathogens (Botryosphaeria berengeriana de Not. f. sp. Parabola Nise Koganezawa et Sakuma), respectively.【Method】The full length cDNA sequence of MhWRKY1 from the M. hupehensis was isolated using the method of in silico cloning combined with the RT-PCR validation. Real-time quantitative RT-PCR (qRT-PCR) was performed to determine the expression pattern of MhWRKY1 in different tissues (leaf, stem and root) and the expression pattern in leaf, stem and root after treatment with SA, MeJA and ACC, respectively. And the expression of this gene after treatment with apple ring spot pathogens was also performed using qRT-PCR.【Result】 1 338 bp full-length cDNA sequence of MhWRKY1 was cloned and the GenBank database accession number was FJ598139. Bioinformatics analysis showed that the open reading frame of this gene was 993 bp, encoding 330 amino acids. The deduced amino acid sequence shares 68%, 68%, 66%, 60%, 59%, 49% and 43% identity with poplar WRKY26, WRKY20, soybean WRKY, potato WRKY2, tobacco WRKY, arabidopsis WRKY7, and rice WRKY53. MhWRKY1 protein contains one conserved WRKY domains that is composed of about 60 amino acid residues with the WRKYGQK sequence followed by a C2H2 zinc finger motif, belongs to the second type of transcription factor. Expression analysis showed that the expression of MhWRKY1 in leaves and stems were high and the expression in leaves and stem were 4.81-fold and 3.75-fold than that in root, respectively. SA, MeJA and ACC induced the expression of MhWRKY1 gene in leave, stem and root, respectively. In addition, the expression of this gene was enhanced and up to the peak at 12 h (9-fold than untreated materials) after treatment with apple ring rots pathogens during the first 72 h.【Conclusion】MhWRKY1 could be involved in SA, MeJA and ET-mediated basic signaling pathway of plant defense responses and disease resistance signal transduction network. MhWRKY1 was involved in defense responses mediated by apple ring rot pathogen. It might be play a key role in disease resistance in M. hupehensis.

    STORAGE·FRESH-KEEPING·PROCESSING
    Effects of Tea Polyphenol on Calcification and Reactive Oxygen Metabolism of Chestnuts
    ZHANG Ying-hao,GU Cai-qin,ZHANG Qian,ZHU Dong-xue,ZHENG Zhi-mao,QIU Hao-chun
    Scientia Agricultura Sinica. 2011, 44(5):  1000-1005 . 
    Abstract ( 715 )   PDF (244KB) ( 494 )   Save
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    【Objective】The effects of antioxidant tea polyphenol on calcification and reactive oxygen species of fresh chestnuts were investigated.【Method】Fresh chestnuts treated with 2%, 4%, or 6% tea polyphenol solutions were stored under room temperature, and then calcification indexes, production rate, H2O2 content, SOD and CAT activities and content of MDA(malondialdehyde) (a membrane lipid peroxidation product of chestnuts) during storage were detected.【Result】 The calcification indexes of chestnuts were significantly inhibited the calcification index of chestnuts. Furthermore, SOD and CAT activities of chestnuts treated with 2% tea polyphenol were higher, while production rate and H2O2 accumulation were lower as compared with chestnuts of control, indicating an inhibition of peroxidation with a lower MDA accumulation.【Conclusion】Treatment with tea polyphenol solution at a suitable concentration reduced active oxygen production and thus inhibited the calcification occurrence of chestnuts during storage.

    Lipase-Catalyzed Synthesis of Diacylglycerol Rich in α-Linolenic Acid from Perilla Seed Oil by Response Surface Methodology
    ZHANG Chao,HU Jiang-ning,FAN Ya-wei,LI Jing,LIU Rong,DENG Ze-yuan
    Scientia Agricultura Sinica. 2011, 44(5):  1006-1014 . 
    Abstract ( 899 )   PDF (454KB) ( 625 )   Save
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    【Objective】Lipase-catalyzed synthesis of DAG production between perilla seed oil and glycerol was studied.【Method】Response surface methodology was used to optimize the reaction conditions of glycerolysis by Lipozyme RM IM in a solvent-free system. 【Result】 The optimal parameters were as follows: substrate molar ratio of glycerol to perilla seed oil was 1﹕1.5, enzyme load was 11.8 wt%, reaction temperature was 72℃, and reaction time was 14.5 h. Under the optimal conditions, DAG content could reach 54.33% in the reaction product, and TAG content lowered to 26.20%. The theoretical purity of DAG oil could reach 67.47% after purification by distillation, and the content of α-linolenic acid reached 52.26% in the DAG oil.【Conclusion】Substrate molar ratio of glycerol to perilla seed oil, enzyme load, reaction temperature and reaction time were all significant factors, and the interaction between substrate molar ratio and enzyme load was the most significant.

    ANIMAL SCIENCE·RESOURCE INSECT
    Screening and Bioinformatics Analysis of ACCase from a BAC Library of Dairy Cow Rumen Microbiota
    ZHAO Sheng-guo,WANG Jia-qi,BU Deng-pan,LIU Kai-lang,ZHU Ya-xin,ZHOU Ling-yun
    Scientia Agricultura Sinica. 2011, 44(5):  1015-1021 . 
    Abstract ( 747 )   PDF (499KB) ( 606 )   Save
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    【Objective】 The objective of the experiment is to screen, sequence and bioinformatical analyze the ACCase clone from a BAC library of the dairy cow rumen microbiota. 【Method】 ACCase gene was screened from BAC library of the rumen microbiota by PCR sequence-driven analysis. Whole insert sequence of screened clone was sequenced by shotgun method. Then it was analyzed by gene annotation, blast and phylogenetic tree analysis. 【Result】 One ACCase clone (U12) was obtained , and its insert fragment (URE12) had the length of 43 358 bp and GC content of 43.75%. URE12 contained 38 CDSs predicted by GeneMark software, and it was probably originated from Proteobacteria by Signature analysis. ACCase encoded by Acc-32 was predicted to be 159 amino acids with a molecular weight of 17.89 kD and pI of 5.27. Acc-32 had the similarity of 41% with that from Elusimicrobium minutum. The combining site AA residue of Acc-32 was GQVICVIEAMKVFNELKA, and Acc-32 belonged to ε-Proteobacteria by phylogenetic tree analysis. 【Conclusion】 The fragment containing ACCase was screened, and ACCase had some novel gene characteristics.

    Cloning and Activity Analysis of Bovine Natural Resistance Associated Macrophage Protein 1 (Nramp1) Gene Promoter
    WANG Hong-mei,ZHANG Li-bo,HOU Ming-hai,WANG Chang-fa,WANG Ling-ling,SUN Tao,HE Hong-bin,ZHONG Ji-feng
    Scientia Agricultura Sinica. 2011, 44(5):  1022-1028 . 
    Abstract ( 661 )   PDF (528KB) ( 875 )   Save
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    【Objective】Bovine natural resistance associated macrophage protein 1 gene (Nramp1) is a major anti-disease candidate gene, but its mechanism of transcriptional regulation remains unclear. To discover the Nramp1 gene expressional mechanism, its promoter region was determined, and the core sequences and major domains were found.【Method】 The 5′flanking region and different fixed 3′ terminal fragments of bovine Nramp1 were cloned and recombined into EGFP-N1 and/or pGL3-basic plasmids. After DNA sequencing confirmation, the recombined vectors were transfected into 293T and murine mammary epithelial cells RAW264.7, respectively, and treated with lipopolysaccharide (LPS), and then the promoter activities of different fragments of 5′flanking region of Nramp1 gene were determined by qualitative and quantitative assays including semi-quantitative RT-PCR and luciferase reporter gene analysis assay. 【Result】 The studies showed that the long DNA fragment, sequence from +58 to -89, and sequence up to -1 748 of 5′flanking region, had the stronger, basal, or maximal promoter activities, respectively. Further studies showed that there were positive (-89/-205 and -278/-1 495) and negative (-205/-278) regulatory domains, respectively. In addition, Escherichia coli LPS significantly enhanced the activity of Nramp1 promoter, and induced its expression in a dose-dependent and cell specific way. 【Conclusion】 The recombinant reporter plasmids containing different deduced fragments of Nramp1 promoter were successfully constructed, and its core and major regulatory regions were found.

    VETERINARY SCIENCE

    Study on Repair of Selenium-Enriched Goose Fatty Liver on Hyperlipemia in Rats

    WANG Wei-wei,WANG Bao-wei,GENG Xin,GE Wen-hua,ZHANG Ming-ai,YUE Bin,WANG Xiao-xiao
    Scientia Agricultura Sinica. 2011, 44(5):  1029-1039 . 
    Abstract ( 701 )   PDF (505KB) ( 870 )   Save
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    【Objective】 The repair effects of selenium-enriched goose fatty liver on hyperlipemia in rats were studied.【Method】Seventy-two Wistar male rats with body weight around 150-180 g were divided into 8 groups including control group, obesity model group, goose fatty liver group (low dose, middle dose, high dose) and selenium-enriched goose fatty liver group (low dose, middle dose, high dose) after 5 days of feeding. Rats in control and obesity model groups were administered with distilled water at the dose of 20 g?kg-1 BW. Rats in low dose, middle dose and high dose groups were administered with goose fatty liver or selenium-enriched goose fatty liver at the doses of 10 g, 20 g, and 3 0 g?kg-1 BW, respectively. Rats in control group were fed with basic feed, and the other seven groups were fed with high fat feeds. The effect of feeds on growth, indexes of lipid metabolism and microscope pathomorphism were analyzed after 8 weeks of feeding. 【Result】 Compared with obesity model group, low dose of goose fatty liver reduced kidney surrounding fat significantly (P<0.05). Low dose of selenium-enriched goose fatty liver reduced kidney surrounding fat ratio, testicle surrounding fat amount and testicle surrounding fat ratio, body fat amount and body fat ratio significantly (P<0.05). The body length of low dose group of selenium-enriched goose fatty liver was significant lower (P<0.05) than that of the low dose group of goose fatty liver. Compared with obesity model group, low dose of goose fatty liver reduced serum triglycerides content (TG) significantly (P<0.05). Middle dose of goose fatty liver reduced the content of TG, total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) (P<0.05) and arteriosclerosis index (AI) significantly (P<0.01). High dose of goose fatty liver reduced the content of LDL-C and AI significantly (P<0.05). Low and middle doses of selenium-enriched goose fatty liver reduced the content of TG, TC, LDL-C (P<0.05) and AI significantly (P<0.01). High dose of selenium-enriched goose fatty liver reduced the content of LDL-C (P<0.05) and AI significantly (P<0.01). Compared with low dose group of goose fatty liver, low dose of selenium-enriched goose fatty liver reduced AI significantly (P<0.01). Compared with obesity model group, low and middle doses of goose fatty liver increased the activity of total lipase (LA) (P<0.05), high dose of goose fatty liver could enhanced the activities of hepatic lipase (HL) and LA (P<0.05).Low and middle doses of selenium-enriched goose fatty liver increased the activity of lipoprotein lipase (LPL) and LA (P<0.05), and reduced the content of lipofuscin (LP) (P<0.05). High dose of selenium-enriched goose fatty liver increased the activities of LA, LPL and HL (P<0.05). Compared with low dose group of goose fatty liver, low dose of selenium-enriched goose fatty liver increased the activity of LPL and LA (P<0.05) Compared with middle dose group of goose fatty liver, middle dose of selenium-enriched goose fatty liver increased the activity of LPL(P<0.05) and LA (P<0.01). Compared with high dose group of goose fatty liver, high dose of selenium-enriched goose fatty liver increased the activity of LPL (P<0.05). The liver adipose degeneration was ameliorated in some degree in all groups of goose fatty liver and selenium-enriched goose fatty liver, especially in low and middle group.【Conclusion】Both goose fatty liver and selenium-enriched goose fatty liver can improve lipid metabolism and have significant effects on decreasing blood fat and on resisting arteriosclerosis and on repairing hepatic steatosis of rats. Selenium-enriched goose fatty liver is better than goose fatty liver.

    Effects of Dietary Supplemented with Arginine on Growth and Development of Fetus in Pregnant Huanjiang Mini-Pigs
    LIU Jun-feng,WU Chen,KONG Xiang-feng ,YANG Huan-sheng ,LI Feng-na,GENG Mei-mei,WANG Min ,YIN Yu-long ,HE Ruo-gang
    Scientia Agricultura Sinica. 2011, 44(5):  1040-1045 . 
    Abstract ( 639 )   PDF (232KB) ( 697 )   Save
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    【Objective】 The present study was conducted to investigate the effects of arginine (Arg) on growth and development of fetus in Huanjiang mini-pigs. 【Method】 Ten first-parity female Huanjiang mini-pigs with a live body weight (BW) of 35-40 kg were chosen in this study and raised in ten pens of one pig in each. After 15 days of service, the animals were randomly assigned into two groups (5 pigs per group), representing supplementation of 1.71% L-alanine (iso-nitrogenous control) or 0.83% L-Arg. After 45 days of service, the sows were weighted and killed by bloodletting of carotid artery. Blood samples were collected into heparin-coated tubes and the plasma were got by centrifugation. The number, BW, survival rate of fetus were observed, and the plasma, amniotic fluid, allantonic fluid and placenta were collected for analysis. 【Result】 Results showed that the average BW of fetus and corpus luteum number from sow fed Arg-supplemented diet were 14.4% (P<0.05) and 6.0% (P>0.05) higher, while the content of crude fat in placenta was 14.7% lower (P<0.05) than the alanine-supplemented diet group. Dietary addition of Arg increased (P<0.05) Arg concentration in amniotic fluid by 65.0% and allantonic fluid by 79.1%, while decreased (P<0.05) lysine concentration in allantonic fluid by 28.3%. The concentrations of nitric oxide (NO) in plasma and allantonic fluid in sow fed with Arg-supplemented diet increased (P<0.05) by 22.9% and 66.3%, respectively, the activities of total NO synthetase increased by 75.5%, inducible NO synthetase increased by 61.7% and constitutive NO synthetase increased by 84.5% in placenta compared with the control group. 【Conclusion】 These findings suggested that Arg plays an important role in increasing growth and development of fetus by stimulating NO production of placenta during early pregnancy in sow.

    AGRICULTURAL ECONOMY & MANAGMENT
    Research on the Mechanism and Policies of Accumulation of Eco-capital of Green Agriculture
    YAN Li-dong,DENG Yuan-jian,QU Zhi-guang
    Scientia Agricultura Sinica. 2011, 44(5):  1046-1055 . 
    Abstract ( 853 )   PDF (284KB) ( 850 )   Save
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    【Objective】Combining with theory of green agriculture and theory of eco-capital, this paper discusses the mechanism and policies of accumulation of eco-capital of green agriculture. Its purpose is to promote virtuous running of eco-capital of green agriculture and sustainable development of green agriculture.【Method】Guidance under the theory of ecologic economics and agricultural ecological economics, this paper uses the method of technical analysis from the angle of multi-disciplinary subjects to explain the mechanism and policies of accumulating of eco-capital of green agriculture.【Result】Accumulation of eco-capital of green agriculture includes natural accumulation and artificial accumulation. Human factors play an important role in running of eco-capital of green agriculture. The path of accumulation of eco-capital of green agriculture includes agricultural economic ecologization and agricultural ecological economization. The mechanism of accumulation of eco-capital of green agriculture includes mechanism of ecology protection of green agriculture, mechanism of ecology restoration of green agriculture, mechanism of ecology compensation of green agriculture. The policies of accumulation of eco-capital of green agriculture include industrial policy, trade policy, technology policy, investment policy, management policy, and so on.【Conclusion】Accumulation of eco-capital of green agriculture is the base of running of eco-capital of green agriculture, and sound mechanism and policy is the guarantee of the accumulation of eco-capital of green agriculture.

    RESEARCH NOTES
    Genetic Diversity and Similarity of Global Faba Bean (Vcia faba L.) Germplasm Revealed by ISSR Markers
    WANG Hai-fei,GUAN Jian-ping,SUN Xue-lian,MA Yu,ZONG Xu-xiao
    Scientia Agricultura Sinica. 2011, 44(5):  1056-1062 . 
    Abstract ( 837 )   PDF (292KB) ( 685 )   Save
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    【Objective】 The purpose of this research is to analyze the genetic diversity of global faba bean germplasm, to explore their genetic similarity and population structure, and to provide essential information for germplasm evaluation and effective utilization of faba bean genetic resources. 【Method】 The genetic similarity of 383 faba bean accessions from 35 countries was analyzed by using ISSR marker. 【Result】 Eleven ISSR primers generated 229 unambiguous bands, of which 212 were polymorphic, and the rate of polymorphic bands was 0.93. Gene diversity index (H) and allelic richness (NA) of different geographic groups of germplasm ranged from 0.16 to 0.28 and from 104 to 193, respectively. Chinese spring-seeding area group showed the highest genetic diversity (H = 0.28, NA = 193), and America group showed the lowest (H = 0.16, NA = 104). The spring faba bean germplasm was clearly separated from winter faba bean germplasm of China in UPGMA clustering analysis based on ISSR molecular marker data. Germplasm from China is quite distinct to that from exotic accessions. The accessions from Europe had a closer genetic similarity with that from North Africa. While the germplasm resources from Asia, Europe and Africa are closely related to their geographical distribution.【Conclusion】Accessions from spring-seeding area of China were most diverse. Germplasm from America showed lowest diversity. The results indicated that the genetic similarity and diversity of faba bean germplasm are closely associated with their growth habit, their geographical origin and ecological distribution.
    Key words: faba bean (Vcia faba L.); germplasm resourses; ISSR marker; genetic diversity; genetic similarity

    Polymorphism Analysis of H-FABP Gene and Correlation with IMF Content in Wei Pig
    ZHANG Chen-hua,WANG Yang,DING Yue-yun ,YIN Zong-jun
    Scientia Agricultura Sinica. 2011, 44(5):  1063-1070 . 
    Abstract ( 961 )   PDF (596KB) ( 463 )   Save
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    【Objective】 Polymorphism of heart fatty acid-binding protein (H-FABP) gene and its correlation with IMF content in Wei pig were studied. 【Method】 PCR-RFLP (HinfⅠ, HaeⅢ, MspⅠ3 kinds of restriction enzymes) and molecular marker techniques were used in the study and the genetic variation of H-FABP gene 5′-upstream region and second intron in 123 Wei pigs was analyzed. 【Result】 In 5′-upstream region, there was polymorphism in loci of the HinfⅠand the frequency of allele H was 0.6545, showing moderate polymorphism (PIC = 0.3500). In the second intron, polymorphism was detected in polymorphic loci HinfⅠ*, the frequency of allele B was 0.7195, showing moderate polymorphism (PIC = 0.3222). Loci of HaeⅢ also existed, allele D frequency was 0.6301, showing moderate polymorphism (PIC = 0.3575 ). While no polymorphism was detected in the loci of MspⅠ, which the genotype was AA genotype. The gene frequencies and genotype frequencies of pigs tested have reached the Hardy-Weinberg equilibrium (P>0.05). The detection of the genotype of the IMF content had the trend of HH>Hh>hh, dd>Dd>DD, BB>Bb>bb, genetic effect values were as following: 4.6918, 4.2665, 3.7712, 4.6124, 4.3167, 3.8173, 4.3042, 4.2358, and 4.1970. Cloning and sequencing analysis on polymorphic fragments of three PCR-RFLP in H-FABP gene showed that HinfⅠ-RFLP is due to T→C mutation in 1 324 bp, HaeⅢ-RFLP is due to C→G mutation in 1 811 bp, HinfⅠ*- RFLP is due to C→T mutation in 1 970 bp. Sequencing results had 97.9% and 98.3% homology with the sequence X98558 and Y16180 published by GenBank. 【Conclusion】Polymorphisms in 5′-upstream region and the second intron of H-FABP gene had certain effect on IMF content in Wei pig. However, whether these polymorphisms are important genetic markers, which are related with H-FABP gene and IMF content in Wei pig, needs to be further studied.
    Effect of Different Dietary Carbohydrate Structure on Fermentation and Amino Acid Profile of Microorganisms in the Rumen of Goats
    WANG Hong-rong,ZHANG Jie
    Scientia Agricultura Sinica. 2011, 44(5):  1071-1076 . 
    Abstract ( 729 )   PDF (215KB) ( 490 )   Save
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    【Objective】 The main objective was to investigate the effect of different dietary carbohydrate structure on fermentation and variation in amino acid profile of microorganisms in the rumen of goats, and to provide evidence for a global debate whether amino acids profile in the rumen microbes is constant or not.【Method】Four Xuhuai goats fitted with permanent rumen cannulas were used in a 4×4 Latin Square design experiment. Diets based on corn, soybean meal and rice straw were divided into 4 treatments according to ratio of nonstructural and structural carbohydrates (A: 0.33, B: 0.63, C: 1.17, D: 2.18). The experiment was conducted to determine the effect of dietary NSC/SC on rumen fermentation patterns and the amino acid profile variation of rumen microbes.【Result】 With the increase of dietary NSC/SC, there were some tendencies that the rumen pH decreased significantly (P<0.05), NH3-N concentration in rumen increased significantly (P<0.05). The percent of acetate decreased, the percent of propionate increased, and the ratio of acetate to propionate decreased in rumen significantly (P<0.05) corresponding with the increase of dietary NSC/SC. Some amino acids concentration of rumen bacteria varied significantly (P<0.05), such as Asp, Ser, Gly, Val, Met, Leu, Phe, His, Arg, and Pro. Different NSC/SC ratios also affected some protozoa amino acids, such as Asp, Ala, Leu, Phe, Lys, His, Arg, and Pro. 【Conclusion】 It is concluded that amino acid profile derived from rumen microbes is mainly variable with dietary carbohydrate structure in term of NSC/SC ratio.