Abstract:

【Objective】 To determine the acaricidal bioactivities of Artemisia annua against Panonychus citri,so to provide a scientific basis for the comprehensive development and utilization of A. annua. 【Method】 Collect The whole A. annua plants were collected in June and July respectively, and the whole plants were cut into root, stems and leaves, petroleum ether (30-60℃), petroleum ether (60-90℃), ethanol, acetone and water were used and extracted by the parallel and sequenced solvents extraction methods, finally 54 kinds of extracts were obtained and their indoor acaricidal bioactivity against P. citri were determined. 【Results】 The results showed that the biological activity of the A. annua plant in July was better than in June and the acetone parallel extract of leaf in July was stronger than other extracts. The LC50 of the acetone parallel extract of leaf in July was 0.4222 mg?ml-1, which was only 44% of 0.9489 mg?ml-1 in June. With the bio-guided isolation method, the acaricidal activity of different components isolated from A. annua July leaf parallel acetone extracts by column chromatography were determined in the laboratory. The 20 kinds of components were obtained, the acaricidal activity of component 17 was the strongest. There was a significant difference between component 17 and other components (P＜0.05). 【Conclusion】 The acetone parallel extract of leaf in July showed a strongest biological activity against P. citri. And separated by column chromatography, 20 kinds of components were obtained, component 17 exhibited a highest acaricidal bioactivity and certain acaricidal bioactivity compound would be identified in further studies.

Key words: Artemisia annua, Panonychus citri, acaricidal bioactivity