Abstract: 【Objective】In order to establish an experimental system for annfaf gene function study in strawberry.【Method】Utilizing anti-sense technology, annfaf gene which was cloned from strawberry fruit, was inserted directionally into pROKⅡ, a plant intermediate expression vector, to construct an anti-sense fusion gene and a plant expression vector pRRJ4. Genetic transformation to strawberry was mediated by EHA105. Transgenic assays were performed using PCR and Southern blot.【Result】The anti-sense fusion annfaf gene had been integrated into strawberry genome.【Conclusion】An experimental system for selecting deficient transgenic plants which might benefit for further study on the mechanism of maturation of non-climacteric fruits such as strawberry was established. Also these deficient plants can be used as intermediate materials for fresh-keeping strawberry breeding.

Key words: Strawberry (Fragaria×ananassa Duch.), Anti-sense gene of annfaf, Plant expression vector, Transgenic plants