Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (7): 1331-1336 .doi: 10.3864/j.issn.0578-1752.at-2006-7621

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES • Previous Articles     Next Articles

Construction of Full-length cDNA Libraries from Root and Shoot of Ae.tauschii and EST Annotation and Comparative Analysis

GuangYao Zhao   

  1. 中国农业科学院作物科学研究所/农业部作物种质资源与生物技术重点开放实验室；国家农作物基因资源与基因改良重大科学工程
  • Received:2006-05-24 Revised:2006-10-08 Online:2007-07-10 Published:2007-07-10
  • Contact: GuangYao Zhao

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Abstract: Abstract: Expressed sequenced tags (EST) and full-length cDNA were important tool and resources for biological functional genomic and genetic research., the progenitor for D genome of bread wheat, was an important material for wheat genetic and genomic research.. Two full-length cDNA libraries were constructed from shoot and root of Aegilops tauschii Y2282 respectively by use of Cap-trapper method and 6616 ESTs and 6973 ESTs were obtained from the 2 libraries respectively. By use of our bioinformatic analysis platform base on Linux system ， we did electronic annotation for the EST, including blasting against public database such as protein ,nucleotide and triticum and Aegilops EST databases from NCBI. By use of result of GO ontology catalogue ,which was obtained from software GoPipee, and expression profile ,which were come from Phrap assembling result, some EST differently or specifically expressed in 2 cDNA libraries were obtained. Maybe the results could help us understand the molecular basis of different functions which organs perform.

Key words: Aegilops taushii, EST, Full-length cDNA library, Function annotation, Bioinformatics

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