Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (6): 1244-1254.doi: 10.3864/j.issn.0578-1752.2026.06.008

• PLANT PROTECTION • Previous Articles     Next Articles

Analysis of the Lethal Activity of Serine Carboxypeptidase DmSCBP1 from Dionaea muscipula Against Bactrocera dorsalis

LIU Jia1,2(), ZHOU ShiQi3, ZHOU Yan1,2, SHEN GuangMao1,2()   

  1. 1 College of Plant Protection, Southwest University, Chongqing 400715
    2 Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing 400715
    3 HanHong College, Southwest University, Chongqing 400715
  • Received:2025-11-25 Accepted:2025-12-22 Online:2026-03-16 Published:2026-03-24
  • Contact: SHEN GuangMao

RichHTML

0

PDF

47

Abstract:

【Background】The long-term use of chemical pesticides has led to a series of problems such as drug resistance and environmental residue. Therefore, the green control of pest requires safer and more efficient active substances. Plants are the largest source of active substances of natural products for pest control. Carnivorous plants have the ability to capture and digest insects, and the components of their secreted digestive fluids exhibit potential insecticidal activity. 【Objective】The purpose of this study is to identify the protein components in the digestive fluid of Dionaea muscipula, and to screen out the proteins with insecticidal activity by combining the transcriptome data of D. muscipula after being induced by prey. 【Method】The adults of Bactrocera dorsalis were put into the trap to observe and record the process of digestion, and confirm the digestive ability of the trap. By analyzing the transcriptome data of the traps and glands of D. muscipula, genes with stress expression in response to prey stimulation were identified. Combined with the proteomic sequencing of the digestive fluid within the traps, the proteases that play a crucial role in the digestion process were clarified. Recombinant proteins were expressed in Escherichia coli in vitro, and injected into or dripped onto B. dorsalis adult to test insecticidal activity of recombinant proteins. 【Result】By observing the process of D. muscipula digesting B. dorsalis, it was found that the D. muscipula has the ability to digest B. dorsalis. The secreted digestive fluid can damage the body wall of B. dorsalis, and it takes about 10 to 13 days to digest a B. dorsalis. Following enzymatic digestion, only the epidermal layer is left. After being stimulated by the prey, DNA-binding transcription factors, serine carboxypeptidase, calmodulin and K+ uptake protein were simultaneously identified in the differentially expressed genes of the transcriptome and proteome datasets, and they may play an important role in the process of digesting prey by D. muscipula. The expression levels of serine carboxypeptidase genes DmSCBP1, DmSCBP2, DmSCBP5, DmSCBP8 in the traps and glands significantly increased after being stimulated by the prey. Among them, the expression level of DmSCBP1 was the most significantly increased, and the protein sequence of this gene was also detected in the protein sequencing of the digestive fluid. The recombinant protein of DmSCBP1 was expressed through the prokaryotic expression system. The biological assay results showed that this protein had no contact toxicity on B. dorsalis, but the injection method could cause a high mortality, and the mortality increased with the increase of time and dose within a certain range. 【Conclusion】The recombinant DmSCBP1 has lethal activity against the B. dorsalis, but its effective usage methods need further improvement.

Key words: Dionaea muscipula, Bactrocera dorsalis, biopesticide, serine carboxypeptidase, insecticidal activity

Fig. 1

The process of D. muscipula digesting B. dorsalis"

Fig. 2

Key genes identified in the transcriptome of traps and glands and sequenced proteins in the secretion of D. muscipula"

Fig. 3

Phylogenetic tree and expression information of serine carboxypeptidase genes from D. muscipula"

Fig. 4

Protein sequence information of DmSCBP1 from D. muscipula"

Fig. 5

Lethal effect of recombinant DmSCBP1 on B. dorsalis by injection"

[1]
杨琴, 杨大全, 柴永飞, 杨毅娟, 龚占斌, 谭安超, 张秀英, 王顺富, 王斌, 李云国, 等. 橘小实蝇食饵剂筛选及田间抗性检测. 应用昆虫学报, 2025, 62(2): 506-513.
YANG Q, YANG D Q, CHAI Y F, YANG Y J, GONG Z B, TAN A C, ZHANG X Y, WANG S F, WANG B, LI Y G, et al. Optimizing baits for Bactrocera dorsalis and detecting insecticide resistance in a population of this pest in Yunnan. Chinese Journal of Applied Entomology, 2025, 62(2): 506-513. (in Chinese)
[2]
JIN T, ZENG L, LIN Y Y, LU Y Y, LIANG G W. Insecticide resistance of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), in mainland China. Pest Management Science, 2011, 67(3): 370-376.

doi: 10.1002/ps.v67.3
[3]
胡维月, 李振, 李凯, 李振亚, 席玉强, 尹新明. 橘小实蝇成虫的防治药剂筛选及抗性监测. 河南农业大学学报, 2023, 57(2): 269-276, 287.
HU W Y, LI Z, LI K, LI Z Y, XI Y Q, YIN X M. Screening of insecticides and resistance monitoring of Bactrocera dorsalis adults. Journal of Henan Agricultural University, 2023, 57(2): 269-276, 287. (in Chinese)
[4]
ABUQAMAR S F, EL-SAADONY M T, ALKAFAAS S S, ELSALAHATY M I, ELKAFAS S S, MATHEW B T, ALJASMI A N, ALHAMMADI H S, SALEM H M, EL-MAGEED T A, et al. Ecological impacts and management strategies of pesticide pollution on aquatic life and human beings. Marine Pollution Bulletin, 2024, 206: 116613.

doi: 10.1016/j.marpolbul.2024.116613
[5]
ALBASEER S S, JASPERS V L B, ORSINI L, VLAHOS P, AL-HAZMI H E, HOLLERT H. Beyond the field: How pesticide drift endangers biodiversity. Environmental Pollution, 2025, 366: 125526.

doi: 10.1016/j.envpol.2024.125526
[6]
张天竞, 杨旭, 周倩, 张涵钦, 刘蕊, 杨海荣, 袁野, 邢立国. 噻虫嗪对3种常见熊蜂单头工蜂的急性毒性. 农药, 2023, 62(9): 647-650.
ZHANG T J, YANG X, ZHOU Q, ZHANG H Q, LIU R, YANG H R, YUAN Y, XING L G. Acute toxicity and safety evaluation of thiamethoxam to 3 common bumblebee monocephalic workers. Agrochemicals, 2023, 62(9): 647-650. (in Chinese)
[7]
邱一蕾, 吴帆, 张莉, 李红亮. 亚致死剂量吡虫啉对中华蜜蜂神经代谢基因表达的影响. 中国农业科学, 2022, 55(8): 1685-1694. doi: 10.3864/j.issn.0578-1752.2022.08.018.
QIU Y L, WU F, ZHANG L, LI H L. Effects of sublethal doses of imidacloprid on the expression of neurometabolic genes in Apis cerana cerana. Scientia Agricultura Sinica, 2022, 55(8): 1685-1694. doi: 10.3864/j.issn.0578-1752.2022.08.018. (in Chinese)
[8]
刘京国, 赵晓萌, 杨爱珍, 师光禄. 含有α-胰凝乳蛋白酶作用位点的Cry3A突变体的构建、表达、纯化以及杀虫活性分析. 中国农业科学, 2011, 44(7): 1384-1389. doi: 10.3864/j.issn.0578-1752.2011.07.010.
LIU J G, ZHAO X M, YANG A Z, SHI G L. Construction, expression, purification and insecticidal activity analysis of Cry3A mutant with α-chymotrypsin site. Scientia Agricultura Sinica, 2011, 44(7): 1384-1389. doi: 10.3864/j.issn.0578-1752.2011.07.010. (in Chinese)
[9]
SPARKS T C, SPARKS J M, DUKE S O. Natural product-based crop protection compounds─Origins and future prospects. Journal of Agricultural and Food Chemistry, 2023, 71: 2259-2269.

doi: 10.1021/acs.jafc.2c06938
[10]
EL-SAYED A M, BYERS J A, SUCKLING D M. Pollinator-prey conflicts in carnivorous plants: When flower and trap properties mean life or death. Scientific Reports, 2016, 6: 21065.

doi: 10.1038/srep21065
[11]
SCHAEFER H M, RUXTON G D. Fatal attraction: Carnivorous plants roll out the red carpet to lure insects. Biology Letters, 2008, 4(2): 153-155.

doi: 10.1098/rsbl.2007.0607 pmid: 18198137
[12]
BENNETT K F, ELLISON A M. Nectar, not colour, may lure insects to their death. Biology Letters, 2009, 5(4): 469-472.

doi: 10.1098/rsbl.2009.0161 pmid: 19429649
[13]
MITHOFER A. Carnivorous plants and their biotic interactions. Journal of Plant Interactions, 2022, 17(1): 333-343.

doi: 10.1080/17429145.2022.2038710
[14]
ADAMEC L, MATUSÍKOVÁ I, PAVLOVIC A. Recent ecophysiological, biochemical and evolutional insights into plant carnivory. Annals of Botany, 2021, 128(3): 241-259.

doi: 10.1093/aob/mcab071 pmid: 34111238
[15]
HEDRICH R, FUKUSHIMA K. On the origin of carnivory: Molecular physiology and evolution of plants on an animal diet. Annual Review of Plant Biology, 2021, 72: 133-153.

doi: 10.1146/annurev-arplant-080620-010429 pmid: 33434053
[16]
HEDRICH R, KREUZER I. Demystifying the Venus flytrap action potential. New Phytologist, 2023, 239(6): 2108-2112.

doi: 10.1111/nph.19113 pmid: 37424515
[17]
DURAK G M, SPECK T, POPPINGA S. Shapeshifting in the Venus flytrap (Dionaea muscipula): Morphological and biomechanical adaptations and the potential costs of a failed hunting cycle. Frontiers in Plant Science, 2022, 13: 970320.

doi: 10.3389/fpls.2022.970320
[18]
SCHERZER S, SHABALA L, HEDRICH B, FROMM J, BAUER H, MUNZ E, JAKOB P, AL-RASCHEID K A S, KREUZER I, BECKER D, et al. Insect haptoelectrical stimulation of Venus flytrap triggers exocytosis in gland cells. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(18): 4822-4827.
[19]
PROCKO C, RADIN I, HOU C, RICHARDSON R A, HASWELL E S, CHORY J. Dynamic calcium signals mediate the feeding response of the carnivorous sundew plant. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119(30): e2206433119.
[20]
JAKSOVA J, ADAMEC L, PETRIK I, NOVAK O, SEBELA M, PAVLOVIC A. Contrasting effect of prey capture on jasmonate accumulation in two genera of aquatic carnivorous plants (Aldrovanda, Utricularia). Plant Physiology and Biochemistry, 2021, 166: 459-465.

doi: 10.1016/j.plaphy.2021.06.014
[21]
PAVLOVIČ A, KOLLER J, VROBEL O, CHAMRÁD I, LENOBEL R, TARKOWSKI P. Is the co-option of jasmonate signalling for botanical carnivory a universal trait for all carnivorous plants? Journal of Experimental Botany, 2024, 75(1): 334-349.

doi: 10.1093/jxb/erad359
[22]
LI Y X, CHEN A, LEU W M. Sessile trichomes play major roles in prey digestion and absorption, while stalked trichomes function in prey predation in Byblis guehoi. International Journal of Molecular Sciences, 2023, 24(6): 5305.

doi: 10.3390/ijms24065305
[23]
PLACHNO B J, KAPUSTA M, STOLARCZYK P, SWIATEK P. Do cuticular gaps make it possible to study the composition of the cell walls in the glands of Drosophyllum lusitanicum? International Journal of Molecular Sciences, 2024, 25(2): 1320.

doi: 10.3390/ijms25021320
[24]
PLACHNO B J, KAPUSTA M, STOLARCZYK P, SWIATEK P, LICHTSCHEIDL I. Differences in the occurrence of cell wall components between distinct cell types in glands of Drosophyllum lusitanicum. International Journal of Molecular Sciences, 2023, 24(20): 15045.

doi: 10.3390/ijms242015045
[25]
SICKEL W, VAN DE WEYER A L, BEMM F, SCHULTZ J, KELLER A. Venus flytrap microbiotas withstand harsh conditions during prey digestion. FEMS Microbiology Ecology, 2019, 95(3): fiz010.
[26]
MONTERO H, FUKUSHIMA K. Non-prey biotic interactions in carnivorous plants. Current Biology, 2023, 33(11): R497-R500.

doi: 10.1016/j.cub.2023.01.053
[27]
SUN P F, LU M R, LIU Y C, SHAW B J P, LIN C P, CHEN H W, LIN Y, HOH D Z, KE H M, WANG I F, et al. An acidophilic fungus promotes prey digestion in a carnivorous plant. Nature Microbiology, 2024, 9(10): 2522-2537.

doi: 10.1038/s41564-024-01766-y
[28]
KOCAB O, JAKSOVA J, NOVAK O, PETRIK I, LENOBEL R, CHAMRAD I, PAVLOVIC A. Jasmonate-independent regulation of digestive enzyme activity in the carnivorous butterwort Pinguicula × Tina. Journal of Experimental Botany, 2020, 71(12): 3749-3758.

doi: 10.1093/jxb/eraa159
[29]
DKHAR J, BHASKAR Y K, LYNN A, PAREEK A. Pitchers of Nepenthes khasiana express several digestive-enzyme encoding genes, harbor mostly fungi and probably evolved through changes in the expression of leaf polarity genes. BMC Plant Biology, 2020, 20(1): 524.

doi: 10.1186/s12870-020-02663-2
[30]
KOVACIK J, KLEJDUS B, REPCAKOVA K. Phenolic metabolites in carnivorous plants: Inter-specific comparison and physiological studies. Plant Physiology and Biochemistry, 2012, 52: 21-27.

doi: 10.1016/j.plaphy.2011.11.007 pmid: 22305064
[31]
RAJ G, KURUP R, HUSSAIN A A, BABY S. Distribution of naphthoquinones, plumbagin, droserone, and 5-O-methyl droserone in chitin-induced and uninduced Nepenthes khasiana: Molecular events in prey capture. Journal of Experimental Botany, 2011, 62(15): 5429-5436.

doi: 10.1093/jxb/err219
[32]
HATCHER C R, RYVES D B, MILLETT J. The function of secondary metabolites in plant carnivory. Annals of Botany, 2020, 125(3): 399-411.

doi: 10.1093/aob/mcz191 pmid: 31760424
[33]
KRASUSKA U, WAL A, STASZEK P, CIACKA K, GNIAZDOWSKA A. Do reactive oxygen and nitrogen species have a similar effect on digestive processes in carnivorous Nepenthes plants and humans? Biology, 2023, 12(10): 1356.

doi: 10.3390/biology12101356
[34]
WAL A, STASZEK P, PAKULA B, PARADOWSKA M, KRASUSKA U. ROS and RNS alterations in the digestive fluid of Nepenthes x ventrata trap at different developmental stages. Plants, 2022, 11(23): 3304.

doi: 10.3390/plants11233304
[35]
BEMM F, BECKER D, LARISCH C, KREUZER I, ESCALANTE- PEREZ M, SCHULZE W X, ANKENBRAND M, VAN DE WEYER A L, KROL E, AL-RASHEID K A, et al. Venus flytrap carnivorous lifestyle builds on herbivore defense strategies. Genome Research, 2016, 26(6): 812-825.

doi: 10.1101/gr.202200.115 pmid: 27197216
[36]
GRABHERR M G, HAAS B J, YASSOUR M, LEVIN J Z, THOMPSON D A, AMIT I, ADICONIS X, FAN L, RAYCHOWDHURY R, ZENG Q, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology, 2011, 29(7): 644-652.

doi: 10.1038/nbt.1883 pmid: 21572440
[37]
ASHBURNER M, BALL C A, BLAKE J A, BOTSTEIN D, BUTLER H, CHERRY J M, DAVIS A P, DOLINSKI K, DWIGHT S S, EPPIG J T, et al. Gene Ontology: Tool for the unification of biology. Nature Genetics, 2000, 25(1): 25-29.

doi: 10.1038/75556
[38]
TRAPNELL C, WILLIAMS B A, PERTEA G, MORTAZAVI A, KWAN G, VAN BAREN M J, SALZBERG S L, WOLD B J, PACHTER L. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nature Biotechnology, 2010, 28(5): 511-515.

doi: 10.1038/nbt.1621 pmid: 20436464
[39]
SUBRAMANIAN A, TAMAYO P, MOOTHA V K, MUKHERJEE S, EBERT B L, GILLETTE M A, PAULOVICH A, POMEROY S L, GOLUB T R, LANDER E S, et al. Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(43): 15545-15550.
[40]
PARK G W, HWANG H, KIM K H, LEE J Y, LEE H K, PARK J Y, JI E S, PARK S R, YATES J R, KWON K H, et al. Integrated proteomic pipeline using multiple search engines for a proteogenomic study with a controlled protein false discovery rate. Journal of Proteome Research, 2016, 15(11): 4082-4090.

pmid: 27537616
[41]
TAMURA K, DUDLEY J, NEI M, KUMAR S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 2007, 24(8): 1596-1599.

doi: 10.1093/molbev/msm092 pmid: 17488738
[42]
ARNOLD K, BORDOLI L, KOPP J, SCHWEDE T. The SWISS- MODEL workspace: A web-based environment for protein structure homology modelling. Bioinformatics, 2006, 22(2): 195-201.

doi: 10.1093/bioinformatics/bti770
[43]
JAKUBEC D, SKODA P, KRIVAK R, NOVOTNY M, HOKSZA D. PrankWeb 3: Accelerated ligand-binding site predictions for experimental and modelled protein structures. Nucleic Acids Research, 2022, 50(W1): W593-W597.

doi: 10.1093/nar/gkac389 pmid: 35609995
[44]
周奕轩, 张晨夕, 别之龙, 成金桃. 捕蝇草的捕虫机理及应用前景研究进展. 植物生理学报, 2020, 56(10): 2047-2060.
ZHOU Y X, ZHANG C X, BIE Z L, CHENG J T. Research progress on insect trapping mechanism and application prospects of Venus flytrap (Dionaea muscipula). Plant Physiology Journal, 2020, 56(10): 2047-2060. (in Chinese)
[45]
LIBIAKOVA M, FLOKOVA K, NOVAK O, SLOVAKOVA L, PAVLOVIC A. Abundance of cysteine endopeptidase dionain in digestive fluid of Venus flytrap (Dionaea muscipula Ellis) is regulated by different stimuli from prey through jasmonates. PLoS ONE, 2014, 9(8): e104424.

doi: 10.1371/journal.pone.0104424
[46]
KREUZER I, SCOSSA F, TOHGE T, FERNIE A R, HEDRICH R. Venus flytraps’ metabolome analysis discloses the metabolic fate of prey animal foodstock. The Plant Journal, 2025, 123(3): e70391.

doi: 10.1111/tpj.v123.3
[47]
SCHERZER S, KROL E, KREUZER I, KRUSE J, KARL F, VON RÜDEN M, ESCALANTE-PEREZ M, MÜLLER T, RENNENBERG H, AL-RASHEID K A S, et al. The Dionaea muscipula ammonium channel DmAMT1 provides NH4+ uptake associated with Venus flytrap’s prey digestion. Current Biology, 2013, 23(17): 1649-1657.

doi: 10.1016/j.cub.2013.07.028
[48]
LICHTSCHEIDL I, LANCELLE S, WEIDINGER M, ADLASSNIG W, KOLLER-PEROUTKA M, BAUER S, KRAMMER S, HEPLER P K. Gland cell responses to feeding in Drosera capensis, a carnivorous plant. Protoplasma, 2021, 258(6): 1291-1306.

doi: 10.1007/s00709-021-01667-5
[49]
RAVEE R, SALLEH F I M, GOH H H. Discovery of digestive enzymes in carnivorous plants with focus on proteases. PeerJ, 2018, 6: e4914.

doi: 10.7717/peerj.4914
[50]
JAKSOVA J, LIBIAKOVA M, BOKOR B, PETRIK I, NOVAK O, PAVLOVIC A. Taste for protein: Chemical signal from prey stimulates enzyme secretion through jasmonate signalling in the carnivorous plant Venus flytrap. Plant Physiology and Biochemistry, 2020, 146: 90-97.

doi: S0981-9428(19)30470-X pmid: 31734521
[51]
叶玲飞, 罗光宇, 向建华, 刘爱玲, 陈信波. 丝氨酸羧肽酶及其类蛋白的研究进展. 植物生理学报, 2013, 49(2): 122-126.
YE L F, LUO G Y, XIANG J H, LIU A L, CHEN X B. Research progress of serine carboxypeptidases and serine carboxypeptidase-like proteins. Plant Physiology Journal, 2013, 49(2): 122-126. (in Chinese)
[52]
ZHANG D Z, ZHAO Y Q, WANG J Z, ZHAO P, BRS1 mediates plant redox regulation and cold responses. BMC Plant Biology, 2021, 21(1): 268.

doi: 10.1186/s12870-021-03045-y pmid: 34116634
[53]
CHEN J, LI W, JIA Y. The serine carboxypeptidase-like gene SCPL41 negatively regulates membrane lipid metabolism in Arabidopsis thaliana. Plants, 2020, 9(6): 696.

doi: 10.3390/plants9060696
[54]
LI Z Y, TANG L Q, QIU J H, ZHANG W, WANG Y F, TONG X H, WEI X J, HOU Y X, ZHANG J. Serine carboxypeptidase 46 regulates grain filling and seed germination in rice (Oryza sativa L.). PLoS ONE, 2016, 11(7): e0159737.

doi: 10.1371/journal.pone.0159737
[55]
DONG H L, YANG L, LIU Y L, TIAN G F, TANG H, XIN S S, CUI Y X, XIONG Q, WAN H F, LIU Z, et al. Detection of new candidate genes controlling seed weight by integrating gene coexpression analysis and QTL mapping in Brassica napus L. Crop Journal, 2023, 11(3): 842-851.

doi: 10.1016/j.cj.2022.09.009
[56]
QI X L, DONG Y X, LIU C L, SONG L L, CHEN L, LI M. A 5.2-kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase- like enhances fruit firmness in Prunus avium. Plant Biotechnology Journal, 2024, 22(6): 1622-1635.

doi: 10.1111/pbi.v22.6
[57]
KENNEY E, YAPARLA A, HAWDON J M, O’HALLORAN D M, GRAYFER L, ELEFTHERIANOS I. A putative lysozyme and serine carboxypeptidase from Heterorhabditis bacteriophora show differential virulence capacities in Drosophila melanogaster. Developmental and Comparative Immunology, 2021, 114: 103820.

doi: 10.1016/j.dci.2020.103820
[58]
许再福. 普通昆虫学. 北京: 科学出版社, 2009: 65-68.
XU Z F. General Entomology. Beijing: Science Press, 2009: 65-68. (in Chinese)
[59]
曹伟平, 丰硕, 程佳旭, 陈丹, 吴青君, 宋健. 布氏白僵菌菌株JG-17的鉴定及对3种金龟子的毒力测定. 中国农业科学, 2024, 57(12): 2364-2377. doi: 10.3864/j.issn.0578-1752.2024.12.008.
CAO W P, FENG S, CHENG J X, CHEN D, WU Q J, SONG J. Species identification of Beauveria brongniartii strain JG-17 and virulence against three Scarabaeoidae pests. Scientia Agricultura Sinica, 2024, 57(12): 2364-2377. doi: 10.3864/j.issn.0578-1752.2024.12.008. (in Chinese)
[60]
FANG W G, LU H L, KING G F, ST LEGER R J. Construction of a hypervirulent and specific mycoinsecticide for locust control. Scientific Reports, 2014, 4: 7345.

doi: 10.1038/srep07345 pmid: 25475694
[61]
DE-THIER J S, PYATI P, BELL J, READSHAW J J, BROWN A P, FITCHES E C. Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA. Microbial Cell Factories, 2023, 22(1): 157.

doi: 10.1186/s12934-023-02176-1
[62]
SUKIRAN N A, PYATI P, WILLIS C E, BROWN A P, READSHAW J J, FITCHES E C. Enhancing the oral and topical insecticidal efficacy of a commercialized spider venom peptide biopesticide via fusion to the carrier snowdrop lectin (Galanthus nivalis agglutinin). Pest Management Science, 2023, 79(1): 284-294.

doi: 10.1002/ps.v79.1
[63]
XUE Q, SWEVERS L, TANING C N T. Plant and insect virus-like particles: Emerging nanoparticles for agricultural pest management. Pest Management Science, 2023, 79(9): 2975-2991.

doi: 10.1002/ps.v79.9
[1] SUN Zheng, LAI ZhongXiao, ZHAO XiaoMin, JIANG ZhiLi, CHEN GuangYou, MA ZhiQing. Application Evaluation of the Whole-Process Biological Management Scheme for Apple Pests in the Weibei Dry Highland [J]. Scientia Agricultura Sinica, 2023, 56(6): 1102-1112.
[2] GUAN RuoBing,LI HaiChao,MIAO XueXia. Commercialization Status and Existing Problems of RNA Biopesticides [J]. Scientia Agricultura Sinica, 2022, 55(15): 2949-2960.
[3] LIU Jing-guo, ZHAO Xiao-meng, YANG Ai-zhen, SHI Guang-lu. Construction, Expression, Purification and Insecticidal Activity Analysis of Cry3A Mutant with α-Chymotrypsin Site [J]. Scientia Agricultura Sinica, 2011, 44(7): 1384-1389.
[4] ZHANG Shu,WANG Min,HAN Mei-lin,CHEN Qiang,MA Rong-cai,GAO Jun-lian
. Study on Synthesis of HrpNEcc Protein in Recombinant Escherichia coli Induced by Yeast Extract
[J]. Scientia Agricultura Sinica, 2009, 42(11): 3880-3887 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd