Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (12): 2570-2580.doi: 10.3864/j.issn.0578-1752.2021.12.008

• PLANT PROTECTION • Previous Articles     Next Articles

A New Integrated Soil Disinfection Machine Improves the Uniformity of Dazomet in Soil

FANG WenSheng1(),CAO AoCheng1(),WANG QiuXia1,YAN DongDong1,LI Yuan1,JIN Xi2,ZHAO QiLong3,4,QIU YaoKang5,ZHAO HongMing5   

  1. 1Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193
    2Joint Center of Soil Remediation of Baoding University and Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Baoding 071000, Hebei
    3Anhui Chunhui Ecological Environment Technology Co. LTD, Wuhu 241000, Anhui
    4Chunhui (Shanghai) Agricultural Science and Technology Development Co., LTD, Shanghai 200135
    5Jiangsu Nantong Shizhuang Chemical Co. LTD, Nantong 226000, Jiangsu
  • Received:2020-08-20 Accepted:2020-09-25 Online:2021-06-16 Published:2021-06-24
  • Contact: AoCheng CAO E-mail:fws0128@163.com;caoac@vip.sina.com

Abstract:

【Objective】Dazomet is the granular soil fumigant with the largest applied area and used amount in China, which has excellent control effect on soil-borne pathogens, nematode, underground insects and weeds. However, dazomet application through hand can easily lead to drug drift and uneven soil distribution, which significantly reducing the fumigation effects. Therefore, it is high time to find an efficient and safe application technology of dazomet. In this study, an integrated soil disinfection machine was applied to increase the depth of pesticide application and improve the distribution uniformity of soil layer of dazomet.【Method】The field fumigation was combined with indoor culture experiments to evaluate the distribution uniformity of the fumigant in different depth layers (0-40 cm) as well as the control effects on Fusarium and Phytophthora following dazomet fumigation by a new integrated soil disinfection machine (NIM). The headspace sampling and GC-MS were used to investigate the vertical distribution characteristics of gas methyl isothiocyanate (MITC) and residual MITC in soil at different depths of 0-40 cm. The specific culture medium was used to isolate the soil-borne pathogens of Fusarium and Phytophthora in soil, and then the occurrence of pathogenic bacteria at different depths of 0-40 cm were analyzed.【Result】The fumigation tests in Anhui, Shanxi and Hebei all showed that, under the model of machine application, fumigant dazomet was evenly distributed in 0-40 cm deep soil layer, the concentration of MITC in gas and in soil was 1.46-3.02 and 18.67-26.27 μg/g soil, respectively, and there was no significant difference in MITC solubility among different deep soil layers. However, in the hand-sprinkling mode, dazomet was mainly distributed in 0-10 cm deep soil layer, and no MITC was detected in 20-40 cm deep soil layer. In addition, MITC solubility in 20-40 cm deep soil layer could not be increased by increasing dazomet application dosage under the hand-sprinkling mode, but MITC solubility in 0-10 cm deep soil layer under the hand-sprinkling mode and 0-40 cm deep soil layer under the machine application mode was significantly increased. Furthermore, the results showed that the decrease rate of Fusarium and Phytophthora at 0-40 cm depth was 90%-100% under machine application mode, while the decrease rate at 20-40 cm depth was less than 43% under hand-sprinkling mode. Even though the decrease rate of Fusarium and Phytophthora was as much as 90%-100% at 0-10 cm depth under hand-sprinkling mode. There was no significant difference on Phytophthora control effect between 40 and 60 g·m-2 dazoment application dosage.【Conclusion】The new soil disinfection machine in this study can effectively improve dazomst soil distribution uniformity and increase the prevention and control effects of soil pathogens in the deep soil layer. The pesticide content at each depth soil layer was significantly increased under the machine application mode when dazoment application was increased.

Key words: soil fumigation, dazomet, disinfecting apparatus, soil-borne disease, methyl isothiocyanate (MITC)

Fig. 1

Schematic diagram of the new integrated soil disinfection machine"

Fig. 2

Field fumigation drawing of the new integrated soil disinfection machine and the traditional hand-sprinkling mode"

Table 1

Physical and chemical properties of experimental plots"

含水量
Water content
(%)
pH
(1﹕2.5)
电导率
Conductivity
(μs·cm-1)
硝态氮
NO3--N
(mg·kg-1)
铵态氮
NH4+-N
(mg·kg-1)
有效磷
Available phosphorus
(mg·kg-1)
速效钾
Available potassium
(mg·kg-1)
有机质
Organic matter
(g·kg-1)
山西运城
Yuncheng, Shanxi
16.2 8.4 289.5 16.1 2.4 150.1 259.3 20.0
河北满城
Mancheng, Hebei
29.6 7.8 350.0 36.0 1.6 381.1 490.6 18.0
安徽长丰
Changfeng, Anhui
23.4 7.3 314.6 36.9 10.1 404.2 422.5 12.3

Fig. 3

Comparison of gas solubility of MITC in different depth soil layers (5-40 cm) under two application methods"

Fig. 4

MITC solubility at depths of 5-40 cm under machine application mode"

Fig. 5

Soil solubility of MITC in different depths (5-40 cm) under two application methods"

Table 2

Count and decrease rate of Fusarium and Phytophthora in different soil depths under dazomet hand-sprinkling and machine application"

安徽Anhui 山西Shanxi 河北Hebei
CK 手撒-40
Hand-40
机施-40
Machine-40
CK 手撒-40
Hand-40
机施-40
Machine-40
CK 手撒-40
Hand-40
机施-40
Machine-40
手撒-60
Hand-60
机施-60
Machine-60
镰孢菌
Fusarium
5 cm 1600±120 0 0 100±20 0 0 0 0 0 0 0
10 cm 1740±135 0 0 140±35 0 0 0 0 0 0 0
20 cm 1440±127 0 0 140±27 80±25
(43%)
0 0 0 0 0 0
30 cm 600±120 450±20
(25%a)
0 100±20 120±22
(-20%)
0 0 0 0 0 0
40 cm 500±130 370±14
(26%a)
0 100±30 165±45
(-65%)
0 0 0 0 0 0
疫霉菌
Phytophthora
5 cm 3600±302 0 0 1600±200 0 0 1920±230 0 0 0 0
10 cm 6520±250 590±310
(91%a)
0 1520±105 0 0 1880±405 0 0 0 0
20 cm 7920±270 2748±280
(65%b)
145±25
(98%a)
1920±205 500±60
(74%a)
0 1700±106 600±45
(65%a)
133±11
(92%a)
578±22
(66%a)
137±22
(92%a)
30 cm 1660±350 1660±210
(0%d)
140±35
(92%a)
660±50 600±80
(1%c)
0 1660±115 853±40
(48%b)
138±25
(92%a)
984±35
(41%b)
158±45
(90%a)
40 cm 1000±330 780±320
(22%c)
0 1000±140 800±50
(20%b)
0 1050±112 670±30
(36%c)
102±12
(90%a)
596±23
(43%b)
28±18
(97%a)
[1] JEFFRIES M D, GANNON T W, REYNOLDS W C, YELVERTON F H, SILCOX C A. Herbicide applications and incorporation methods affect dazomet efficacy on bermudagrass. HortTechnology, 2017,27(1):24-29.
doi: 10.21273/HORTTECH03564-16
[2] FRAEDRICH S W, DWINELL L D. An evaluation of dazomet incorporation methods on soilborne organisms and pine seedling production in southern nurseries. Southern Journal of Applied Forestry, 2003,27(1):41-51.
doi: 10.1093/sjaf/27.1.41
[3] MAO L G, WANG Q X, YAN D D, XIE H W, LI Y, GUO M X, CAO A C. Evaluation of the combination of 1,3-dichloropropene and dazomet as an efficient alternative to methyl bromide for cucumber production in China. Pest Management Science, 2012,68(4):602-609.
doi: 10.1002/ps.2303
[4] SHI W, LI M, WEI G, TIAN R, LI C, WANG B, LIN R, SHI C, CHI X, ZHOU B, GAO Z. The occurrence of potato common scab correlates with the community composition and function of the geocaulosphere soil microbiome. Microbiome, 2019,7:14.
doi: 10.1186/s40168-019-0629-2
[5] 曹坳程, 郭美霞, 王秋霞, 李园, 颜冬冬. 世界土壤消毒技术进展. 中国蔬菜, 2010(21):17-22.
CAO A C, GUO M X, WANG Q X, LI Y, YAN D D. Progress of soil disinfection technology in the world. Chinese Vegetables, 2010(21):17-22. (in Chinese)
[6] 曹坳程, 王久臣. 土壤消毒原理与应用. 北京: 科学出版社, 2015.
CAO A C, WANG J C. Principle and Application of Soil Fumigation. Beijing: Science Press, 2015. (in Chinese)
[7] 毛连纲. 新型熏蒸剂的毒力评价及应用技术研究[D]. 北京: 中国农业科学院, 2015.
MAO L G. Toxicity evaluation of new fumigants and research on the application technology[D]. Beijing: Chinese Academy of Agricultural Sciences, 2015. (in Chinese)
[8] FU C H, HU B Y, CHANG T T, HSUEH K L, HSU W T. Evaluation of dazomet as fumigant for the control of brown root rot disease. Pest Management Science, 2012,68(7):959-962.
doi: 10.1002/ps.v68.7
[9] PRIDER J, WILLIAMS A. Using dazomet to reduce broomrape seed banks in soils with low moisture content. Crop Protection, 2014,59:43-50.
doi: 10.1016/j.cropro.2014.01.013
[10] HUSSAIN M, MAŇASOVÁ M, ZOUHAR M, RYŠÁNEK P. Comparative virulence assessment of different nematophagous fungi and chemicals against northern root-knot nematodes, Meloidogyne hapla, on carrots. Pakistan Journal of Zoology, 2020,52(1):199-206.
[11] MAO L G, JIANG H Y, WANG Q X, YAN D D, CAO A C. Efficacy of soil fumigation with dazomet for controlling ginger bacterial wilt (Ralstonia solanacearum) in China. Crop Protection, 2017,100:111-116.
doi: 10.1016/j.cropro.2017.06.013
[12] MAO L G, YAN D D, WANG Q X, LI Y, OUYANG C B, LIU P F, SHEN J, GUO M X, CAO A C. Evaluation of the combination of dimethyl disulfide and dazomet as an efficient methyl bromide alternative for cucumber production in China. Journal of Agricultural and Food Chemistry, 2014,62(21):4864-4869.
doi: 10.1021/jf501255w
[13] LIN C M, PRESTON 3rd J F, WEI C I. Antibacterial mechanism of allyl isothiocyanate. Journal of Food Protection, 2000,63(6):727-734.
doi: 10.4315/0362-028X-63.6.727
[14] ROBERTS T R, HUTSON D H. Metabolic Pathways of Agrochemicals. Part 2: Insecticides and Fungicides. Royal Society of Chemistry Cambridge, UK, 1999: 1221-1224.
[15] FANG W S, WANG Q X, YAN D D, HUANG B, REN Z J, WANG Q, SONG Z X, LIU X M, LI Y, OUYANG C B, CAO A C. Environmental factors and soil amendment affect the decomposition rate of dazomet fumigant. Journal of Environment Quality, 2018,47(5):1223-1231.
doi: 10.2134/jeq2018.01.0003
[16] CONSOLAZIO N, LOWRY G V, KARAMALIDIS A K. Hydrolysis and degradation of dazomet with pyrite: Implications for persistence in produced waters in the Marcellus Shale. Applied Geochemistry, 2019,108:104383.
doi: 10.1016/j.apgeochem.2019.104383
[17] 方文生. 生物炭对异硫氰酸甲酯降解/活性的影响与机制[D]. 北京: 中国农业科学院, 2016.
FANG W S. Effects and mechanism of biochar on methyl isothiocyanate of degradation and boiactivity[D]. Beijing: Chinese Academy of Agricultural Sciences, 2016. (in Chinese)
[18] 鲍士旦. 土壤农化分析. 3版. 北京: 中国农业出版社, 2018.
BAO S D. Soil Agrochemical Analysis. 3rd ed. Beijing: China Agriculture Press, 2018. (in Chinese)
[19] WANG Q X, GAO S D, WANG D, SPOKAS K, CAO A C, YAN D D. Mechanisms for 1,3-dichloropropene dissipation in biochar-amended soils. Journal of Agricultural and Food Chemistry, 2016,64(12):2531-2540.
doi: 10.1021/acs.jafc.5b04941
[20] FANG W S, WANG Q X, HAN D W, LIU P F, HUANG B, YAN D D, OUYANG C B, LI Y, CAO A C. The effects and mode of action of biochar on the degradation of methyl isothiocyanate in soil. The Science of the Total Environment, 2016,565:339-345.
doi: 10.1016/j.scitotenv.2016.04.166
[21] KOMADA H. Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soil. Review of Plant Protection Research, 1975,8:114-124.
[22] MASAGO H, YOSHIKAWA M, FUKADA M. Selective inhibition of Pythium spp. on a medium for direct isolation of Phytophthora spp. from soils and plants. Phytopathology, 1977,67(3):425-428.
[23] 蔡祖聪, 黄新琦. 土壤学不应忽视对作物土传病原微生物的研究. 土壤学报, 2016,53(2):305-310.
CAI Z C, HUANG X Q. Soil-borne pathogens should not be ignored by soil science. Acta Pedologica Sinica, 2016,53(2):305-310. (in Chinese)
[24] 黄新琦, 蔡祖聪. 土壤微生物与作物土传病害控制. 中国科学院院刊, 2017,32(6):593-600.
HUANG X Q, CAI Z C. Soil microbes and control of soil-borne diseases. Bulletin of Chinese Academy of Sciences, 2017,32(6):593-600. (in Chinese)
[25] MARTIN F N. Development of alternative strategies for management of soilborne pathogens currently controlled with methyl bromide. Annual Review of Phytopathology, 2003,41:325-350.
doi: 10.1146/annurev.phyto.41.052002.095514
[26] 王秋霞, 颜冬冬, 王献礼, 吕平香, 李雄亚, 曹坳程. 土壤熏蒸剂研究进展. 植物保护学报, 2017,44(4):529-543.
WANG Q X, YAN D D, WANG X L, LÜ P X, LI X Y, CAO A C. Research advances in soil fumigants. Journal of Plant Protection, 2017,44(4):529-543. (in Chinese)
[27] MAO L G, WANG Q X, YAN D D, MA T T, LIU P F, SHEN J, LI Y, OUYANG C B, GUO M X, CAO A C. Evaluation of chloropicrin as a soil fumigant against Ralstonia solanacarum in ginger (Zingiber officinale Rosc.) production in China. PLoS ONE, 2014,9(3):e91767.
doi: 10.1371/journal.pone.0091767
[28] WANG D, YATES S R, GAO S. Chloropicrin emissions after shank injection: Two-dimensional analytical and numerical model simulations of different source methods and field measurements. Journal of Environment Quality, 2011,40(5):1443-1449.
doi: 10.2134/jeq2010.0233
[29] WANG Q X, MAO L G, WANG D, YAN D D, MA T T, LIU P F, ZHANG C L, WANG R Q, GUO M X, CAO A C. Emission reduction of 1,3-dichloropropene by soil amendment with biochar. Journal of Environment Quality, 2014,43(5):1656-1662.
doi: 10.2134/jeq2014.02.0075
[30] 曹坳程, 刘晓漫, 郭美霞, 王秋霞, 李园, 欧阳灿彬, 颜冬冬. 作物土传病害的危害及防治技术. 植物保护, 2017,43(2):6-16.
CAO A C, LIU X M, GUO M X, WANG Q X, LI Y, OUYANG C B, YAN D D. Incidences of soil-borne diseases and control measures. Plant Protection, 2017,43(2):6-16. (in Chinese)
[1] LIU RuiChi,CHENG YouPu,CHAI ALi,SHI YanXia,XIE XueWen, PATIGULI,LI BaoJu. Establishment and Application of a Triplex PCR Detection System for Vegetable Soil-Borne Pathogens [J]. Scientia Agricultura Sinica, 2019, 52(12): 2069-2078.
[2] LI Sheng-hua,LIU Ke-xing,LIAO Zong-wen
. Method for Simplification of Characteristic Carbon Sources for Biolog Analysis of Soil Microbial Community and Its Application
[J]. Scientia Agricultura Sinica, 2010, 43(3): 523-528 .
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!