低分子量有机酸对植烟土壤酶活性和细菌群落结构的影响

doi:10.3864/j.issn.0578-1752.2015.24.008

Abstract

Abstract: 【Objective】Soil biological properties can reflect soil quality rapidly. The objective of this study is to clarify the effects of the low molecular weight organic acids on soil biological properties, and to provide a scientific basis for improving soil quality. 【Method】According to the amount of low molecular weight organic acids during tobacco early growth period and previous research results, a laboratory experiment was carried out to investigate the effects of six kinds of organic acids (benzoic acid, cinnamic acid, lauric acid, phthalic acid, myristic acid and palmitic acid) whose concentrations were 1, 2 and 3 g·kg^-1 (carbon/soil ratio) on four kinds of soil enzymes activities (soil catalase, alkaline phosphatase, urease and sucrase) and bacterial community structure. Tobacco field soil was cultivated in a dark environment at 25℃ for 30 d and the analysis methods for the bacterial community included the related evolutionary tree, principal co-ordinates analysis (PCoA), the classification of the relative abundance columnar diagram and the species abundance clustering heat maps, and so on. 【Result】Soil sucrase activity with three concentrations of cinnamic acid was lower than that of the control, cinnamic acid had the most inhibitory effect on soil sucrase activity. Soil urease activity with three concentrations of cinnamic acid, phthalic acid and benzoic acid were all lower than that of the control, their inhibitions were stronger. Cinnamic acid and benzoic acid had higher inhibitory effect on soil catalase activity, the value of benzoic with 3 g·kg^-1 concentration was the lowest. Cinnamic acid, phthalic acid and benzoic acid inhibited the soil alkaline phosphatase activity greatly whose three concentrations were lower than that of the control and benzoic acid with 3 g·kg^-1 was the lowest. The classification of the columnar diagram of different samples showed lauric acid with 2 g·kg^-1concentration had bigger differences with the others, contrasted with the 6 others, the content of Cyanophyta, Chloroplast, Streptophyta and Streptophyta- unclassified were the highest at Phylum, Class, Order and Family levels separately, the analysis results were consistent with that of the related evolutionary tree and PCoA. The other 6 treatments had no obvious differences at above the Genus level and all the treatments had differences at the Genus level. 【Conclusion】The concentrations of low molecular weight organic acids had significant effects on soil enzyme activities, and soil bacteria may not be the main reason affecting soil enzyme activities.

Key words: root exudates, low molecular weight organic acid, soil enzyme activity, bacterial community structure

YU Hui-yong, SONG Xiao-li, WANG Shu-sheng, CAO Li-jun, GUO Li, WANG Xiao-li, PENG Gong-yin. Effects of Low Molecular Weight Organic Acids on Soil Enzymes Activities and Bacterial Community Structure[J].Scientia Agricultura Sinica, 2015, 48(24): 4936-4947.

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