蒺藜苜蓿,小G蛋白,ROP,共生互作,根毛生长发育
," /> 蒺藜苜蓿,小G蛋白,ROP,共生互作,根毛生长发育
,"/> Medicago truncatula,small G protein,ROP,symbiotic interaction,development of root hair
,"/> <font face="Verdana">Role of Small GTP-Binding Protein ROP in Medicago truncatula in Symbiosis: 2. Function Analysis of MtROP5 Associated with Development of Root Hair#br# </font>

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (8): 1563-1570 .doi: 10.3864/j.issn.0578-1752.2010.08.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Role of Small GTP-Binding Protein ROP in Medicago truncatula in Symbiosis: 2. Function Analysis of MtROP5 Associated with Development of Root Hair#br#

LIU Wei, CHEN Ai-min, FENG Li-xing, CAO Lian-pu, SUN Jie, WANG Yan-zhang#br#   

  1. (石河子大学农学院)
  • Received:2009-10-13 Revised:2010-01-08 Online:2010-04-15 Published:2010-04-15
  • Contact: WANG Yan-zhang

Abstract:

【Objective】 The function of MtROP5 in Medicago truncatula was studied for establishment of the groundwork to investigate the regulatory mechanism of ROP in symbiotic interaction. 【Method】 The promoter sequence of MtROP5 was obtained using PCR, and the expression vectors containing MtROP5 were constructed. The method of Agrobacterium rhizogenes-mediated root transformation was used to investigate the function of MtROP5. 【Result】 The sequence of MtROP5 promoter that is at about 2.1 kb upstream of the translation origin point was obtained. The results showed that the MtROP5 promoter allowed GUS expression in every part of the root, GUS displayed more abundant expression level in vascular bundle and initials of lateral root than other parts of root. The transcriptional activity of MtROP5 promoter was induced to increase in the transgenic roots after infected by Sinorhizobium meliloti Rm1021. Compared with root hairs of the transgenic root of control, the length of transgenic root hairs of overexpression and constitutively active mutant of MtROP5 increased markedly by 74.6% and 54.8%, respectively, the length of transgenic root hair of RNAi interference of MtROP5 reduced by 47.6%, but the dominantly negative mutant of MtROP5 did not affect the development of root hairs. 【Conclusion】 MtROP5 in M. truncatula regulated the development of root hair, and probably involved in the signal transduction at the early stage of symbiotic interaction between host plant and Rhizobium.

Key words: Medicago truncatula')">Medicago truncatula, small G protein, ROP, symbiotic interaction, development of root hair

[1] CHEN XiaoWei, WANG XiaoLong. Accounting Framework of Carbon Footprint on Integrated Cropping-Breeding Farming System: A Case on Maize-Cow-Recycling Manure Model [J]. Scientia Agricultura Sinica, 2023, 56(2): 314-332.
[2] YANG GaiQing, WANG LinFeng, LI WenQing, ZHU HeShui, FU Tong, LIAN HongXia, ZHANG LiYang, TENG ZhanWei, ZHANG LiJie, REN Hong, XU XinYing, LIU XinHe, WEI YuXuan, GAO TengYun. Study on Milk Quality Based on Circadian Rhythm [J]. Scientia Agricultura Sinica, 2023, 56(2): 379-390.
[3] ZHAO HaiXuan,ZHANG YiTao,LI WenChao,MA WenQi,ZHAI LiMei,JU XueHai,CHEN HanTing,KANG Rui,SUN ZhiMei,XI Bin,LIU HongBin. Spatial Characteristic and Its Factors of Nitrogen Surplus of Crop and Livestock Production in the Core Area of the Baiyangdian Basin [J]. Scientia Agricultura Sinica, 2023, 56(1): 118-128.
[4] ZHANG Wei,YAN LingLing,FU ZhiQiang,XU Ying,GUO HuiJuan,ZHOU MengYao,LONG Pan. Effects of Sowing Date on Yield of Double Cropping Rice and Utilization Efficiency of Light and Heat Energy in Hunan Province [J]. Scientia Agricultura Sinica, 2023, 56(1): 31-45.
[5] FENG XiangQian,YIN Min,WANG MengJia,MA HengYu,CHU Guang,LIU YuanHui,XU ChunMei,ZHANG XiuFu,ZHANG YunBo,WANG DanYing,CHEN Song. Effects of Meteorological Factors on Quality of Late Japonica Rice During Late Season Grain Filling Stage Under ‘Early Indica and Late Japonica’ Cultivation Pattern in Southern China [J]. Scientia Agricultura Sinica, 2023, 56(1): 46-63.
[6] LI YiLing,PENG XiHong,CHEN Ping,DU Qing,REN JunBo,YANG XueLi,LEI Lu,YONG TaiWen,YANG WenYu. Effects of Reducing Nitrogen Application on Leaf Stay-Green, Photosynthetic Characteristics and System Yield in Maize-Soybean Relay Strip Intercropping [J]. Scientia Agricultura Sinica, 2022, 55(9): 1749-1762.
[7] XIE YiTong,ZHANG Fei,SHI Jie,FENG Li,JIANG Li. Effects of Exogenous Sucrose on the Postharvest Quality and Chloroplast of Gynura bicolor D.C [J]. Scientia Agricultura Sinica, 2022, 55(8): 1642-1656.
[8] GOU ZhiWen,YIN Wen,CHAI Qiang,FAN ZhiLong,HU FaLong,ZHAO Cai,YU AiZhong,FAN Hong. Analysis of Sustainability of Multiple Cropping Green Manure in Wheat-Maize Intercropping After Wheat Harvested in Arid Irrigation Areas [J]. Scientia Agricultura Sinica, 2022, 55(7): 1319-1331.
[9] LIU Jiao,LIU Chang,CHEN Jin,WANG MianZhi,XIONG WenGuang,ZENG ZhenLing. Distribution Characteristics of Prophage in Multidrug Resistant Escherichia coli as well as Its Induction and Isolation [J]. Scientia Agricultura Sinica, 2022, 55(7): 1469-1478.
[10] TAN XianMing,ZHANG JiaWei,WANG ZhongLin,CHEN JunXu,YANG Feng,YANG WenYu. Prediction of Maize Yield in Relay Strip Intercropping Under Different Water and Nitrogen Conditions Based on PLS [J]. Scientia Agricultura Sinica, 2022, 55(6): 1127-1138.
[11] ZHAO Ling, ZHANG Yong, WEI XiaoDong, LIANG WenHua, ZHAO ChunFang, ZHOU LiHui, YAO Shu, WANG CaiLin, ZHANG YaDong. Mapping of QTLs for Chlorophyll Content in Flag Leaves of Rice on High-Density Bin Map [J]. Scientia Agricultura Sinica, 2022, 55(5): 825-836.
[12] JIANG JingJing,ZHOU TianYang,WEI ChenHua,WU JiaNing,ZHANG Hao,LIU LiJun,WANG ZhiQin,GU JunFei,YANG JianChang. Effects of Crop Management Practices on Grain Quality of Superior and Inferior Spikelets of Super Rice [J]. Scientia Agricultura Sinica, 2022, 55(5): 874-889.
[13] HUANG JiaQuan,LI Li,WU FengNian,ZHENG Zheng,DENG XiaoLing. Proliferation of Two Types Prophage of ‘Candidatus Liberibacter asiaticus’ in Diaphorina citri and their Pathogenicity [J]. Scientia Agricultura Sinica, 2022, 55(4): 719-728.
[14] ZHANG XueLin, WU Mei, HE TangQing, ZHANG ChenXi, TIAN MingHui, LI XiaoLi, HOU XiaoPan, HAO XiaoFeng, YANG QingHua, LI ChaoHai. Effects of Crop Residue Decomposition on Soil Inorganic Nitrogen and Greenhouse Gas Emissions from Fluvo-Aquic Soil and Shajiang Black Soil [J]. Scientia Agricultura Sinica, 2022, 55(4): 729-742.
[15] YANG BinJuan,LI Ping,HU QiLiang,HUANG GuoQin. Effects of the Mixted-cropping of Chinese Milk Vetch and Rape on Soil Nitrous Oxide Emission and Abundance of Related Functional Genes in Paddy Fields [J]. Scientia Agricultura Sinica, 2022, 55(4): 743-754.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!