Abstract:

The simultaneous expression of multiple genes and genetically modified (GM) food security research have been considered as the research focus for genetic engineering in plants presently. Unlike conventional gene transformation technologies, plant artificial chromosomes provide one solution to the stable expression of multiple transgenes. As plant artificial chromosome segregation is independent of host chromosomes, they provide a platform for accelerating plant breeding and for studying the specific chromatin domains inserted into them. The generation of artificial plant chromosomes and their applications were reviewed in this article.

Key words: plant artificial chromosome, site-specific recombination, minichromosome, gene stacking, genetically modified food security