Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (20): 4309-4316.doi: 10.3864/j.issn.0578-1752.2011.20.020

• VETERINARY SCIENCE • Previous Articles     Next Articles

Effects of Different Immune Status on Blood Biochemical Parameters and Cellular Immune Response in Broiler Chickens

 FENG  Yan, YANG  Xiao-Jun, WANG  Xiao-Fei, QIN  Ding-Kui, YIN  Rui-Qing, YAO  Jun-Hu   

  1. 1.西北农林科技大学动物科技学院
    2.山西农业大学生命科学学院
  • Received:2011-04-23 Online:2011-10-15 Published:2011-07-21

Abstract: 【Objective】 This experiment was conducted to determine the effects of different immune statuses on blood biochemical parameters and cellular immune response of broiler chickens. 【Method】 One hundred and eighty 1-day-old AA broilers were allocated into four groups. Each treatment had 5 replicate cages of 9 broilers per replicate pen. This study will set different immune stress models, including the control group (no vaccines, NV), conventional vaccines group (CV), immune hyperthyroidism group (LPS) and immune suppression group (CYP). The T lymphocytes and subgroups were isolated from periphery blood from immunized and unvaccinated birds and were detected by CD3/CD4/CD8 triple labelling by flow cytometric analysis and MTT assays, respectively.【Result】The results showed that different immunological statuses had no significant effect on feed conversion ratio of broiler chickens among 4 treatment (P>0.05). LPS group and CYP group had significantly lower broiler body weight gain and feed intake than NV group (P<0.05). Serum TP, GLU, and CHLO contents were no significantly different among treatments (P>0.05). On d 21 and 35, ALB of LPS and CYP groups was significantly lower than CV and NV groups (P<0.05), and ALB levels of NV and CV groups were not significantly different (P>0.05). On d 21, α-AGP of LPS group was significantly lower than CV group (P<0.05), BUN of LPS group was significantly higher than NV group (P<0.05). CPY and LPS inhibited the T, B lymphocyte proliferation, and CD4+/CD8+ T cell percentage of LPS group was significantly lower than NV group (P<0.05). 【Conclusion】 Chickens with no vaccines maintained normal growth performance and immune response. LPS stress increased the cellular immune response of chickens. Tolerance of chicken to CYP increased with age.

Key words: AAbroiler, immunestatus, bloodparameters, MTTassay, lymphocytecluster

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