Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3603-3612.doi: 10.3864/j.issn.0578-1752.2011.17.013

• HORTICULTURE • Previous Articles     Next Articles

Development of EST-Derived SSR Markers and Their Application in Strawberry Genetic Diversity Analysis

DONG  Qing-Hua, WANG  Xi-Cheng, ZHAO  Mi-Zhen, SONG  Chang-Nian, GE  An-Jing, WANG  Jing   

  1. 1. 北京农学院植物科学技术学院
    2.南京农业大学园艺学院/江苏省果树品种改良与种苗繁育工程中心
    3. 江苏省农业科学院园艺研究所
  • Received:2010-12-27 Revised:2011-06-01 Online:2011-09-01 Published:2011-06-22

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Abstract: 【Objective】 The objective of this study was to analyze the SSR distribution in strawberry ESTs and to develop new EST-derived SSR markers, and application of EST-SSR markers in strawberry genetic diversity analysis was also validated. 【Method】 A total of 55 750 EST sequences of strawberry were obtained from NCBI. These sequences were screened by using MISA software to search for SSR motifs. Sixty pairs of primers were designed by the software Primer3.0 Plus. The PCR products of these primers were detected by PAGE and some of them were recovered for sequencing. 【Result】 A total of 1 490 SSRs were identified from 1 334 strawberry EST sequences. The dinucleotide, trinucleotide and hexanucleotide repeats were the dominant types with the frequency of 36.17%, 26.51% and 19.87%, respectively. Among the 60 EST-SSR primers, 42 amplified distinct bands and expected products, and 36 were polymorphic. Eleven polymorphic EST-SSR amplicons were validated by resequencing the PCR products. A dendrogram tree generated by the 11 validated polymorphic EST-SSR markers revealed reasonable genetic relationships among 20 strawberry cultivars. 【Conclusion】 It is an efficient and economic way to develop SSR markers from the strawberry EST sequences, and the EST-SSR markers can be used in strawberry genetic analysis.

Key words: strawberry, EST, SSR, genetic diversity

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