Abstract: The Rf3 gene is one of two or more nuclear genes required for fertility restoration in male-sterile S-cytoplasm(CMS-S) male. It's near isogenic lines, Ps and Pf, were developed by anther, which serves as materials for isolating and cloning Rf3 gene by using of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) we had improved in this study. The results showed that there was a special protein RRPⅡ(MW25.2kD,pI5.0) in the leaves of Ps during the period of the meiosis of pollen mother cell. It may be play a role in fertility restoration in CMS-S maize. The further isolation and purification of this protein will be beneficial to clone Rf3 gene and discover the molecular mechanism of fertility expressional model. And, the trends and directions of the joint studies for genomics and proteomics were discussed here.

Key words: Male-sterile S-cytoplasm(CMS-S) male maize, Near isogenic lines, Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), 25.2kD Special protein RRPⅡ subunit