Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (12): 2590-2596 .

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Cloning, Prokaryotic Expression of the Buffalo Mature PrP Gene

  

  1. 湖南科技大学生命科学学院
  • Received:2005-08-08 Revised:2005-12-08 Online:2006-12-10 Published:2006-12-10

Abstract: There are no reports on buffalo PrP till now. For the sake, with the designed primers, the buffalo mature PrP gene was amplified by PCR method. And the recombinant plasmid was constructed,which expresses buffalo mature PrP fusion protein. Then the vector was sequenced. The PrP sequences of the family Bovidae were analyzed and it shows the buffalo mature PrP gene contain five copies of a short, G-C-rich element which encodes the octapeptide,and there are two nonapeptides and three octapeptides.After transformed the positive recombinant plasmids, which designated as pET-32a- BPrP , to BL21 (DE3), and the product was achieved a high expression level. After ultrasonicated, the sediment was isolated and purified. And the product was confirmed by Western Blot.

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