Abstract: 【OBJECTIVE】High temperature resistance trait is very important in cucumber production in summer. In order to higher the efficiency of breeding high temperature resistance cucumber lines, molecular markers associated to this trait was analyzed in this research;【METHOD】Two inbred lines, 863-7 (heat-tolerant) and 863-6 (heat-susceptible), were used to construct F2 population. Bulked segregant analysis and selective genotyping were used to screen the candidate SSR and SRAP markers. The screened markers were analyzed in the F2 population;【RESULTS】Sixteen candidate markers, which possibly associated to the high temperature resistance, were gotten. After analyzed with F2 population, 10 markers including 1 SSR marker and 9 SRAP markers were found to be associated with high temperature resistance QTL, each accounted for 6%~17% of phenotypic variation. Mapmaker analysis revealed that these markers represented 3 QTLs accounting for 32.3% of total phenotypic variation;【CONCLUSION】Molecular markers linked to high temperature resistance QTL were selected in this research. It will lay a foundation for high temperature resistance line breeding in cucumber.