Abstract: Shoot regeneration and rooting from hypocotyl explants of Diospyros.oleifera Cheng were studied. The result showed that modified Murashige and Skoog(MS)[MS(1/2N)] containing 0.1 mg•L-1 indole acetic acid and 2.0 mg•L-1 zeatin was the most optimum for shoot regeneration and the shoot percentage and average shoots number per explant were 81.67% and 5.32±2.05 respectively. As for regenerated shoots rooting, the modified Murashige and Skoog containing 0.5 mg•L-1 both indole acetic acid and indole-3-butyric acid was most effective, in which rooting percentage, average roots number and length per shoot reached up to 100%, 3.17 and 2.43 centimeter respectively. There were many ateral roots at the cut ends and the plantlets growed rapidly and vigorously. After rooting, the regenerated plants were transplant in pots where 96% plants survived after one month.