Abstract: To prepare hapten BFNB of carbofuran,Balb/c mice were immunized by synthesized BFNB-BSA. The splenocytes of immunized mice were fused with Sp2/0 cells and the cultural supernatants of hybridoma cell were screened by indirect ELISA with BFNB-OVA as coated antigen. Purified McAbs was got from ascites fluids deposited by octanoic acid and ammonia sulfate. The specificity and affinity of McAbs were characterized by ELISA.A hybridoma cell line (5D3) secreting anti-carbofuran McAb had been established. The titre of culture medium and ascites were up to 1∶2.048×103 and 1∶1.024×106 respectively. The immunoglobulin class of the McAb was IgG1. McAbs showed a high affinity (Ka5D3＝2.54×109L/mol, IC50=1.9 ng/mL) and minimum cross reactivity for several analogous compounds of carbofuran (7×10-4％ for carbofuranphenol, others less than 10-4％).The specific monoclonal antibody against carbofuran was obtained successfully and this made it possible to establish ELISA for rapid detection of carbofuran.

Key words: Carbofuran, Monoclonal antibodies