长期施肥措施下黑土活性碳组分和碳水解酶的动态变化

doi:10.3864/j.issn.0578-1752.2026.18.011

Abstract

Abstract:

【Objective】This study investigated the dynamics and interrelationships between soil labile carbon fractions and carbon-hydrolyzing enzymes under long-term fertilization practices, aiming to provide a theoretical basis for enhancing black soil fertility and optimizing fertilization strategies in Northeast China.【Method】A long-term fertilization experiment established in 1989 in Gongzhuling, Jilin Province, was utilized. Soil samples were collected from five treatments: no fertilization (CK), nitrogen and potassium fertilization (NK), nitrogen-phosphorus-potassium fertilization (NPK), NPK plus straw return (NPKS), and NPK plus manure (NPKM). Measurements included soil nutrients, activities of amylase (AMY), sucrase (SUC), cellobiohydrolase (CBH), β-1,4-glucosidase (βG), and content of particulate organic carbon (POC), easily oxidizable organic carbon (EOC), dissolved organic carbon (DOC), and microbial biomass carbon (MBC). Data were analyzed using ANOVA, correlation analysis, Mantel tests, and partial least squares path modeling to elucidate changes and underlying linkages.【Result】Compared with CK, the NPKM treatment demonstrated the most comprehensive improvement in soil organic carbon and its active components. Soil organic carbon (SOC) content increased by 6.7 g·kg^-1, while POC, EOC, MBC, and DOC content was significantly increased by 2.5 g·kg^-1, 3.0 g·kg^-1, 97.5 mg·kg^-1, and 14.1 mg·kg^-1, respectively, with the geometric mean of active carbon also rising substantially by 50.1%. Under the NPKS treatment, SOC, POC, and MBC content was also increased significantly by 2.3 g·kg^-1, 1.5 g·kg^-1, and 93.0 mg·kg^-1, respectively. In contrast, both the NPK and NK treatments reduced POC and MBC content, with the geometric mean of active carbon decreasing by 24.7% and 26.8%, respectively. The carbon pool management index increased significantly only under the NPKM treatment by 55.9% compared with CK, while the carbon pool activity decreased significantly by 33.6% under the NK treatment. Regarding enzyme activity, all fertilization treatments enhanced AMY activity compared with CK. Additionally, the NK and NPK treatments significantly increased CBH and βG activities, with the NK treatment showing the greatest increase: CBH rose by 167.3 μmol·g^-1·h^-1 and βG by 457.7 μmol·g^-1·h^-1. In terms of soil nutrients, the NPKM treatment significantly increased total nitrogen (TN), total phosphorus (TP), available phosphorus (AP), and available potassium (AK) content, with increases of 0.72 g·kg^-1, 1.85 g·kg^-1, 148.9 mg·kg^-1, and 402.1 mg·kg^-1, respectively, compared with CK. Conversely, the NK treatment resulted in a significant decrease in pH by 2 units and a reduction in TP content by 0.2 g·kg^-1. Furthermore, the NK treatment increased soil C:P and N:P, while other fertilization treatments generally decreased C:N, C:P, and N:P. Correlation and path analysis indicated that soil TN, TP, AP, and AK directly promoted the accumulation of POC, EOC, and MBC. In contrast, higher C:P and N:P stimulated CBH and βG activities, thereby accelerating carbon decomposition and mineralization, which inhibited the accumulation of POC, EOC, and MBC.【Conclusion】Long-term combined application of chemical fertilizers with organic amendments not only improved soil nutrient content but also increased labile organic carbon by optimizing nutrient balance and modulating carbon-hydrolyzing enzyme activities. Thus, integrating organic manure with fertilizers represents the optimal strategy for enhancing black soil fertility in Northeast China.

Key words: black soil, long-term fertilization, combined organic-inorganic fertilization, carbon cycle enzymes, carbon pool stability, soil organic carbon components

HAN XiaoYu, ZHU YuPing, LIU HongFang, SUN ZhiMei, ZHANG XiuZhi, GAO HongJun, PENG Chang, ZHANG ShuXiang. Dynamics of Black Soil Labile Carbon Fractions and Carbon- Hydrolyzing Enzymes Under Long-Term Fertilization Regimes[J].Scientia Agricultura Sinica, 2026, 59(18): 4092-4104.

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References 56

[1]	Haynes R J. Labile organic matter fractions as central components of the quality of agricultural soils: An overview[J]. Advances in Agronomy, 2005, 85: 221-268.
[2]	樊军, 郝明德. 黄土高原旱地轮作与施肥长期定位试验研究Ⅱ.土壤酶活性与土壤肥力[J]. 植物营养与肥料学报, 2003, 9(2): 146-150.
	Fan J, Hao M D. Study on long-term experiment of crop rotation and fertilization in the Loess Plateau Ⅱ. Relationship between soil enzyme[J]. Plant Nutrition and Fertilizer Science, 2003, 9(2): 146-150. (in Chinese)
[3]	German D P, Weintraub M N, Grandy A S, Lauber C L, Rinkes Z L, Allison S D. Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies[J]. Soil Biology and Biochemistry, 2011, 43(7): 1387-1397. doi: 10.1016/j.soilbio.2011.03.017
[4]	Duval M E, Galantini J A, Martínez J M, Limbozzi F. Labile soil organic carbon for assessing soil quality: Influence of management practices and edaphic conditions[J]. Catena, 2018, 171: 316-326. doi: 10.1016/j.catena.2018.07.023
[5]	史帆, 李文广, 易树生, 杨娜, 陈玉萌, 郑伟, 张雪辰, 李紫燕, 翟丙年. 有机无机肥配施下旱地麦田土壤有机碳组分含量的变化特征[J]. 中国农业科学, 2025, 58(4): 719-732. DOI: 10.3864/j.issn.0578-1752.2025.04.008.
	Shi F, Li W G, Yi S S, Yang N, Chen Y M, Zheng W, Zhang X C, Li Z Y, Zhai B N. The variation characteristics of soil organic carbon fractions under the combined application of organic and inorganic fertilizers[J]. Scientia Agricultura Sinica, 2025, 58(4): 719-732. DOI: 10.3864/j.issn.0578-1752.2025.04.008. (in Chinese)
[6]	徐烨红, 巴雯雯, 王炫清, 陆超, 罗佳, 马艳. 设施土壤溶解性有机质含量和质量对不同碳组分有机肥的响应[J]. 土壤学报. https://link.cnki.net/urlid/32.1119.P.20250402.1543.006.
	Xu Y H, Ba W W, Wang X Q, Lu C, Luo J, Ma Y. Response of dissolved organic matter content and quality in greenhouse soils to the application of organic fertilizers with various carbon components[J]. Acta Pedologica Sinica. https://link.cnki.net/urlid/32.1119.P.20250402.1543.006. (in Chinese)
[7]	Sheoran S, Prakash D, Raj D, Yadav P K, Singh R, Gupta R K, Alamri S, Siddiqui M H, Khan S. Organic manure and fertilizer N management strategies improve soil health at different growth stages of pearl millet under pearl millet-wheat sequence[J]. BMC Plant Biology, 2025, 25(1): 117. doi: 10.1186/s12870-025-06128-2 pmid: 39865269
[8]	孙赫阳, 万忠梅, 刘德燕, 廖霞, 丁维新. 有机肥与无机肥配施对潮土N₂O排放的影响[J]. 环境科学, 2020, 41(3): 1474-1481.
	Sun H Y, Wan Z M, Liu D Y, Liao X, Ding W X. Effect of organic fertilizer and inorganic fertilizer application on N₂O emissions from fluvo-aquic soil in the North China Plain[J]. Environmental Science, 2020, 41(3): 1474-1481. (in Chinese)
[9]	何伟, 王会, 韩飞, 胡国庆, 娄燕宏, 宋付朋, 潘红, 魏猛, 诸葛玉平. 长期施用有机肥显著提升潮土有机碳组分[J]. 土壤学报, 2020, 57(2): 425-445.
	He W, Wang H, Han F, Hu G Q, Lou Y H, Song F P, Pan H, Wei M, Zhuge Y P. Effect of long-term application of organic manure expanding organic carbon fractions in fluvo-aquic soil[J]. Acta Pedologica Sinica, 2020, 57(2): 425-445. (in Chinese)
[10]	张瑞, 张贵龙, 姬艳艳, 李刚, 常泓, 杨殿林. 不同施肥措施对土壤活性有机碳的影响[J]. 环境科学, 2013, 34(1): 277-282.
	Zhang R, Zhang G L, Ji Y Y, Li G, Chang H, Yang D L. Effects of different fertilizer application on soil active organic carbon[J]. Environmental Science, 2013, 34(1): 277-282. (in Chinese)
[11]	Blair G J, Lefroy R, Lisle L. Soil carbon fractions based on their degree of oxidation, and the development of a carbon management index for agricultural systems[J]. Australian Journal of Agricultural Research, 1995, 46(7): 1459-1466. doi: 10.1071/AR9951459
[12]	张嫒, 郑朝霞, 赵志远, 冯天宇, 郑伟, 翟丙年. 有机无机肥长期配施对果园土壤碳库及温室气体排放的影响[J]. 环境科学, 2023, 44(10): 5823-5831.
	Zhang A, Zheng Z X, Zhao Z Y, Feng T Y, Zheng W, Zhai B N. Effects of long-term combined application of organic and inorganic fertilizers on soil carbon pool and greenhouse gas emissions in orchards[J]. Environmental Science, 2023, 44(10): 5823-5831. (in Chinese)
[13]	郭亚军, 邱慧珍, 张玉娇, 张建斌, 王友玲, 张春红, Anning Dominic Kwadwo. 不同施肥方式对马铃薯农田土壤有机碳组分和碳库管理指数的影响[J]. 土壤通报, 2021, 52(4): 912-919.
	Guo Y J, Qiu H Z, Zhang Y J, Zhang J B, Wang Y L, Zhang C H, Anning D K. Effects of four fertilization regimes on soil organic carbon fractions and carbon pool management index of potato farmland[J]. Chinese Journal of Soil Science, 2021, 52(4): 912-919. (in Chinese)
[14]	冯继广, 张秋芳, 袁霞, 朱彪. 氮磷添加对土壤有机碳的影响: 进展与展望[J]. 植物生态学报, 2022, 46(8): 855-870. doi: 10.17521/cjpe.2021.0358
	Feng J G, Zhang Q F, Yuan X, Zhu B. Effects of nitrogen and phosphorus addition on soil organic carbon: Review and prospects[J]. Chinese Journal of Plant Ecology, 2022, 46(8): 855-870. (in Chinese) doi: 10.17521/cjpe.2021.0358
[15]	滕臻, 曹小青, 孙孟瑶, 李培玺, 徐小牛. 不同生态恢复模式对巢湖湖滨湿地土壤活性碳库及其管理指数的影响[J]. 生态环境学报, 2019, 28(4): 752-760. doi: 10.16258/j.cnki.1674-5906.2019.04.014
	Teng Z, Cao X Q, Sun M Y, Li P X, Xu X N. Effect of different ecological restoration patterns on soil labile organic carbon and carbon pool management index of lakeside wetland of Chaohu Lake[J]. Ecology and Environment Sciences, 2019, 28(4): 752-760. (in Chinese) doi: 10.16258/j.cnki.1674-5906.2019.04.014
[16]	Geisseler D, Scow K M. Long-term effects of mineral fertilizers on soil microorganisms-A review[J]. Soil Biology and Biochemistry, 2014, 75: 54-63. doi: 10.1016/j.soilbio.2014.03.023
[17]	Spohn M, Klaus K, Wanek W, Richter A. Microbial carbon use efficiency and biomass turnover times depending on soil depth- Implications for carbon cycling[J]. Soil Biology and Biochemistry, 2016, 96: 74-81. doi: 10.1016/j.soilbio.2016.01.016
[18]	章磊, 徐祎萌, 白美霞, 周燕, 秦华, 徐秋芳, 陈俊辉. 生物质炭配施有机物料对红壤碳组分及酶生态化学计量特征的影响[J]. 浙江农林大学学报, 2024, 41(3): 506-516.
	Zhang L, Xu Y M, Bai M X, Zhou Y, Qin H, Xu Q F, Chen J H. Effects of biochar combined with organic amendments on carbon composition and eco-enzymatic stoichiometry of red soil[J]. Journal of Zhejiang A&F University, 2024, 41(3): 506-516. (in Chinese)
[19]	Burns R G, Deforest J L, Marxsen J, Sinsabaugh R L, Stromberger M E, Wallenstein M D, Weintraub M N, Zoppini A. Soil enzymes in a changing environment: Current knowledge and future directions[J]. Soil Biology and Biochemistry, 2013, 58: 216-234. doi: 10.1016/j.soilbio.2012.11.009
[20]	Kalbitz K, Schwesig D, Schmerwitz J, Kaiser K, Haumaier L, Glaser B, Ellerbrock R, Leinweber P. Changes in properties of soil-derived dissolved organic matter induced by biodegradation[J]. Soil Biology and Biochemistry, 2003, 35(8): 1129-1142. doi: 10.1016/S0038-0717(03)00165-2
[21]	Fontaine S, Mariotti A, Abbadie L. The priming effect of organic matter: A question of microbial competition[J]. Soil Biology and Biochemistry, 2003, 35(6): 837-843. doi: 10.1016/S0038-0717(03)00123-8
[22]	Waring B G, Weintraub S R, Sinsabaugh R L. Ecoenzymatic stoichiometry of microbial nutrient acquisition in tropical soils[J]. Biogeochemistry, 2014, 117(1): 101-113. doi: 10.1007/s10533-013-9849-x
[23]	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
	Bao S D. Soil and Agricultural Chemistry Analysis[M]. 3rd ed. Beijing: China Agriculture Press, 2000. (in Chinese)
[24]	Cambardella C A, Elliott E T. Particulate soil organic-matter changes across a grassland cultivation sequence[J]. Soil Science Society of America Journal, 1992, 56(3): 777-783. doi: 10.2136/sssaj1992.03615995005600030017x
[25]	Lefroy R D B, Blair G J, Strong W M. Changes in soil organic matter with cropping as measured by organic carbon fractions and ¹³C natural isotope abundance[J]. Plant and Soil, 1993, 155(1): 399-402.
[26]	Vance E D, Brookes P C, Jenkinson D S. An extraction method for measuring soil microbial biomass C[J]. Soil Biology and Biochemistry, 1987, 19(6): 703-707. doi: 10.1016/0038-0717(87)90052-6
[27]	关松荫. 土壤酶及其研究法[M]. 北京: 农业出版社, 1986.
	Guan S Y. Soil Enzyme and Its Research Method[M]. Beijing: Agriculture Press, 1986. (in Chinese)
[28]	季若彤, 解雪峰, 贾振毅, Cambule Armindo Henrique, 缪源卿, 徐梓晴, 田再洋. 耐盐水稻种植对滨海盐渍土壤有机碳库及碳转化酶活性的影响[J]. 环境科学. https://doi.org/10.13227/j.hjkx.202411085.
	Ji R T, Xie X F, Jia Z Y, Henrique C A, Miao Y Q, Xu Z Q, Tian Z Y. Effects of salt-tolerant rice cultivation on organic carbon pool and carbon conversion enzyme activities in coastal saline soil[J]. Environmental Science. https://doi.org/10.13227/j.hjkx.202411085. (in Chinese)
[29]	钟泽坤. 增温和降雨改变对黄土丘陵区撂荒草地土壤碳循环关键过程的影响[D]. 杨凌: 西北农林科技大学, 2021.
	Zhong Z K. Effects of experimental warming and precipitation manipulation on the key processes of soil carbon cycle in abandoned farmland of the Loess Hilly Region[D]. Yangling: Northwest A&F University, 2021. (in Chinese)
[30]	Zhou G P, Fan K K, Gao S J, Chang D N, Li G L, Liang T, Liang H, Li S, Zhang J D, Che Z X, Cao W D. Green manuring relocates microbiomes in driving the soil functionality of nitrogen cycling to obtain preferable grain yields in thirty years[J]. Science China Life Sciences, 2024, 67(3): 596-610. doi: 10.1007/s11427-023-2432-9
[31]	Chivenge P, Vanlauwe B, Six J. Does the combined application of organic and mineral nutrient sources influence maize productivity: A meta-analysis[J]. Plant and Soil, 2011, 342(1): 1-30. doi: 10.1007/s11104-010-0626-5
[32]	Kirkby C A, Richardson A E, Wade L J, Batten G D, Blanchard C, Kirkegaard J A. Carbon-nutrient stoichiometry to increase soil carbon sequestration[J]. Soil Biology and Biochemistry, 2013, 60: 77-86. doi: 10.1016/j.soilbio.2013.01.011
[33]	Pagliari P H, Laboski C A M. Investigation of the inorganic and organic phosphorus forms in animal manure[J]. Journal of Environmental Quality, 2012, 41(3): 901-910. doi: 10.2134/jeq2011.0451 pmid: 22565271
[34]	Sigurdarson J J, Svane S, Karring H. The molecular processes of urea hydrolysis in relation to ammonia emissions from agriculture[J]. Reviews in Environmental Science and Bio/Technology, 2018, 17(2): 241-258. doi: 10.1007/s11157-018-9466-1
[35]	Guo J H, Liu X J, Zhang Y, Shen J L, Han W X, Zhang W F, Christie P, Goulding K W T, Vitousek P M, Zhang F S. Significant acidification in major Chinese croplands[J]. Science, 2010, 327(5968): 1008-1010. doi: 10.1126/science.1182570 pmid: 20150447
[36]	Chen R R, Senbayram M, Blagodatsky S, Myachina O, Dittert K, Lin X G, Blagodatskaya E, Kuzyakov Y. Soil C and N availability determine the priming effect: Microbial N mining and stoichiometric decomposition theories[J]. Global Change Biology, 2014, 20(7): 2356-2367. doi: 10.1111/gcb.12475 pmid: 24273056
[37]	Lü K M, Li X Z, Chen K Q, Wu B, He B F, Schenk G. Improving the catalytic efficiency of a GH5 processive endoglucanase by a combinatorial strategy using consensus mutagenesis and loop engineering[J]. ACS Catalysis, 2024, 14(9): 6856-6867. doi: 10.1021/acscatal.4c01083
[38]	Fujii K, Inagaki Y, Hayakawa C, Ono K. Decoupling of cellulose decomposition and glucose mineralization in volcanic forest soils[J]. Soil Science and Plant Nutrition, 2023, 69(3): 199-208. doi: 10.1080/00380768.2023.2175178
[39]	郭超, 盛茂银, 何宇, 王霖娇, 石庆龙, 罗娜娜. 土地利用方式对西南喀斯特土壤碳、氮、磷化学计量特征及酶活性的影响[J]. 土壤通报, 2023, 54(2): 382-391.
	Guo C, Sheng M Y, He Y, Wang L J, Shi Q L, Luo N N. Effects of land use types on soil carbon, nitrogen, phosphorus stoichiometric characteristics and enzyme activities in the karst area of southwest China[J]. Chinese Journal of Soil Science, 2023, 54(2): 382-391. (in Chinese)
[40]	Cotrufo M F, Wallenstein M D, Boot C M, Denef K, Paul E. The Microbial Efficiency-Matrix Stabilization (MEMS) framework integrates plant litter decomposition with soil organic matter stabilization: Do labile plant inputs form stable soil organic matter[J]. Global Change Biology, 2013, 19(4): 988-995. doi: 10.1111/gcb.2013.19.issue-4
[41]	李天娇, 张乃于, 申文艳, 宋天昊, 刘红芳, 刘晓燕, 张秀芝, 彭畅, 杨劲峰, 张淑香. 长期施肥对黑土和棕壤团聚体稳定性的影响及驱动因素[J]. 中国农业科学, 2024, 57(19): 3835-3847. DOI: 10.3864/j.issn.0578-1752.2024.19.010.
	Li T J, Zhang N Y, Shen W Y, Song T H, Liu H F, Liu X Y, Zhang X Z, Peng C, Yang J F, Zhang S X. Effects of long-term fertilization on soil aggregate stability and its driving factors in black soil and brown soil[J]. Scientia Agricultura Sinica, 2024, 57(19): 3835-3847. DOI: 10.3864/j.issn.0578-1752.2024.19.010. (in Chinese)
[42]	Kuzyakov Y. Priming effects: Interactions between living and dead organic matter[J]. Soil Biology and Biochemistry, 2010, 42(9): 1363-1371. doi: 10.1016/j.soilbio.2010.04.003
[43]	李金垚, 潘雯, 王佳, 薛亮, 张显松, 李生. 土壤活性有机碳及碳库管理指数对石漠化治理措施的响应[J]. 林业科学研究, 2022, 35(5): 156-163.
	Li J Y, Pan W, Wang J, Xue L, Zhang X S, Li S. Response of active organic carbon and carbon pool management index of karst soil to rocky desertification control measures[J]. Forest Research, 2022, 35(5): 156-163. (in Chinese)
[44]	哈文秀, 周金星, 庞丹波, 关颖慧, 崔明. 岩溶区不同恢复方式下土壤有机碳组分及酶活性研究[J]. 北京林业大学学报, 2019, 41(2): 1-11.
	Ha W X, Zhou J X, Pang D B, Guan Y H, Cui M. Soil organic carbon fraction and enzyme activities under different restoration methods in karst area[J]. Journal of Beijing Forestry University, 2019, 41(2): 1-11. (in Chinese)
[45]	于贵瑞, 高扬, 王秋凤, 刘世荣, 申卫军. 陆地生态系统碳-氮-水循环的关键耦合过程及其生物调控机制探讨[J]. 中国生态农业学报, 2013, 21(1): 1-13.
	Yu G R, Gao Y, Wang Q F, Liu S R, Shen W J. Discussion on the key processes of carbon-nitrogen-water coupling cycles and biological regulation mechanisms in terrestrial ecosystem[J]. Chinese Journal of Eco-Agriculture, 2013, 21(1): 1-13. (in Chinese)
[46]	宁川川, 王建武, 蔡昆争. 有机肥对土壤肥力和土壤环境质量的影响研究进展[J]. 生态环境学报, 2016, 25(1): 175-181. doi: 10.16258/j.cnki.1674-5906.2016.01.026
	Ning C C, Wang J W, Cai K Z. The effects of organic fertilizers on soil fertility and soil environmental quality: A review[J]. Ecology and Environmental Sciences, 2016, 25(1): 175-181. (in Chinese)
[47]	高鹏, 崔新卫, 聂新星, 朱校奇, 鲁耀雄, 彭福元. 中药渣有机肥施用对土壤理化性质及枳壳产质量的影响[J]. 中国土壤与肥料, 2022(10): 73-80.
	Gao P, Cui X W, Nie X X, Zhu X Q, Lu Y X, Peng F Y. Effects of Chinese medicinal residue organic fertilizer application on soil physicochemical properties and Aurantii fructus yield and quality[J]. Soil and Fertilizer Sciences in China, 2022(10): 73-80. (in Chinese)
[48]	王旭, 李斐, 赵世翔. 冻融交替对土壤CO₂排放影响的研究进展[J]. 土壤通报, 2022, 53(3): 728-737.
	Wang X, Li F, Zhao S X. Freeze-thaw regime effects on soil CO₂ emission: A review[J]. Chinese Journal of Soil Science, 2022, 53(3): 728-737. (in Chinese)
[49]	Joshi R, Kasi M, Wadhawan T, Khan E. Production and removal of soluble organic nitrogen by nitrifying biofilm[J]. Journal of Environmental Chemical Engineering, 2021, 9(4): 105440. doi: 10.1016/j.jece.2021.105440
[50]	Ghosh B N, Meena V S, Alam N M, Dogra P, Bhattacharyya R, Sharma N K, Mishra P K. Impact of conservation practices on soil aggregation and the carbon management index after seven years of maize-wheat cropping system in the Indian Himalayas[J]. Agriculture, Ecosystems & Environment, 2016, 216: 247-257. doi: 10.1016/j.agee.2015.09.038
[51]	于兆丰, 方明晴, 唐硕, 孙铭轩, 欧阳俊芃. 苏北县域农田土壤碳库的剖面变化特征分析[J]. 地理科学研究, 2022, 11(3): 290-295.
	Yu Z F, Fang M Q, Tang S, Sun M X, Ouyang J P. Study on profile change characteristics of farmland soil carbon pool in northern Jiangsu[J]. Geographical Science Research, 2022, 11(3): 290-295. (in Chinese) doi: 10.12677/GSER.2022.113030
[52]	王传杰, 王齐齐, 徐虎, 高洪军, 朱平, 徐明岗, 张文菊. 长期施肥下农田土壤-有机质-微生物的碳氮磷化学计量学特征[J]. 生态学报, 2018, 38(11): 3848-3858.
	Wang C J, Wang Q Q, Xu H, Gao H J, Zhu P, Xu M G, Zhang W J. Carbon, nitrogen, and phosphorus stoichiometry characteristics of bulk soil, organic matter, and soil microbial biomass under long-term fertilization in cropland[J]. Acta Ecologica Sinica, 2018, 38(11): 3848-3858. (in Chinese)
[53]	Sinsabaugh R L, Hill B H, Follstad Shah J J. Ecoenzymatic stoichiometry of microbial organic nutrient acquisition in soil and sediment[J]. Nature, 2009, 462(7274): 795-798. doi: 10.1038/nature08632
[54]	Allison S D, Vitousek P M. Responses of extracellular enzymes to simple and complex nutrient inputs[J]. Soil Biology and Biochemistry, 2005, 37(5): 937-944. doi: 10.1016/j.soilbio.2004.09.014
[55]	申文艳. 长期施肥下黑土磷转化微生物特征及其对磷组分的影响[D]. 北京: 北京农学院, 2025.
	Shen W Y. Microbial characteristics of phosphorus transformation in black soil under long-term fertilization and their effects on phosphorus components[D]. Beijing: Beijing Agricultural University, 2025. (in Chinese)
[56]	Manzoni S, Taylor P, Richter A, Porporato A, Ågren G I. Environmental and stoichiometric controls on microbial carbon-use efficiency in soils[J]. The New Phytologist, 2012, 196(1): 79-91. doi: 10.1111/nph.2012.196.issue-1

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处理 Treatment	化肥投入 Inorganic fertilizer input N-P-K (kg∙hm^-2)	有机物料投入 Organic material input N-P-K (kg∙hm^-2)
CK	0-0-0	0-0-0
NK	165-0-68	0-0-0
NPK	165-36-68	0-0-0
NPKS	112-36-68	53-6-77
NPKM	50-36-68	115-39-77

处理	酸碱度 pH	全氮 TN (g∙kg^-1)	全磷 TP (g∙kg^-1)	全钾 TK (g∙kg^-1)	有效磷 AP (mg∙kg^-1)	速效钾 AK (mg∙kg^-1)	碳氮比 C:N	碳磷比 C:P	氮磷比 N:P
CK	7.76±0.32a	1.25±0.04cd	1.03±0.04c	20.61±0.45a	7.39±0.51d	191.30±8.77b	11.87±0.78a	14.45±1.47b	1.22±0.06b
NK	5.76±0.13d	1.36±0.02bc	0.84±0.05d	20.54±0.60a	8.41±1.16d	208.03±10.48b	11.00±0.15b	17.74±1.01a	1.61±0.08a
NPK	6.44±0.14c	1.22±0.04d	1.13±0.04c	20.61±0.28a	26.22±0.91b	202.01±8.92b	10.95±0.07b	11.80±0.34c	1.08±0.02c
NPKS	7.92±0.04a	1.45±0.08b	1.51±0.05b	20.58±0.16a	17.46±1.1c	209.71±6.05b	11.85±0.28a	11.32±0.44c	0.96±0.04d
NPKM	7.30±0.19b	1.97±0.12a	2.88±0.13a	21.22±0.16a	156.33±10.42a	255.89±17.57a	10.91±0.19b	7.47±0.24d	0.68±0.01e

Dynamics of Black Soil Labile Carbon Fractions and Carbon- Hydrolyzing Enzymes Under Long-Term Fertilization Regimes

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