Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (15): 3352-3373.doi: 10.3864/j.issn.0578-1752.2026.15.009

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Cover Crop Species and Incorporation Ratios on Soil Fertility and Economic Returns in Salinized Cotton Fields

WANG YuXia1(), LUO ChuXin2, LI ZiShuang1, DU MengYang1, LI HongJie1, ZHOU XiaoLin1, LIU BaiLin1, CAO WeiDong3, LI ZiZhong2()   

  1. 1 Dezhou Academy of Agricultural Sciences, Dezhou 253000, Shandong
    2 College of Land Science and Technology, China Agricultural University, Beijing 100193
    3 Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2025-10-15 Accepted:2026-01-05 Online:2026-08-01 Published:2026-08-03
  • Contact: LI ZiZhong

Abstract:

【Objective】Aiming at the soil degradation problems (including low organic matter content, nutrient imbalance, and secondary salinization) caused by long-term continuous cropping of cotton fields in northern Shandong, this study aimed to identify the suitable catch crop species and optimal incorporation ratio for salinized cotton fields in this region, so as to provide the technical support for ecological restoration and green production of cotton fields.【Method】The experiment was conducted at the experimental demonstration base in Wucheng County, Dezhou City, Shandong Province from 2022 to 2023. A split-plot design was adopted, with the main plots being three catch crop species: Orychophragmus violaceus, Vicia villosa Roth, and Lolium perenne L., and the subplots being four incorporation ratios: 100%, 70%, 30%, and 0. Winter fallow bare land was used as the control (CK). The biomass and nutrient accumulation of catch crops were systematically determined before incorporation, and soil physicochemical properties (such as available phosphorus, available potassium, alkali-hydrolyzable nitrogen, organic matter, pH, and electrical conductivity), microbial biomass carbon and nitrogen, and activities of key soil enzymes (such as urease, sucrase, and catalase) were dynamically monitored at the seedling, flowering, and harvest stages of cotton. The comprehensive soil fertility index (IFI) was calculated using the fuzzy mathematics membership function combined with principal component analysis, and a comprehensive evaluation was conducted by integrating cotton yield and annual economic benefits.【Result】(1) There were significant differences in soil improvement functions among different catch crops (P<0.05): Vicia villosa Roth had the highest total nitrogen accumulation (70.4 kg·hm-2), showing the best performance in improving soil nitrogen supply and comprehensive fertility; Lolium perenne L. had the highest fresh weight (31 951.4 kg·hm-2), dry weight (6 058.0 kg·hm-2), and total carbon accumulation (3 129.9 kg·hm-2), with significant advantages in carbon input and biomass accumulation; Orychophragmus violaceus had stable nutrient release and balanced overall performance. (2) The incorporation ratio significantly regulated soil indicators (P<0.05), and the 100% incorporation treatment performed the best. Compared with CK, it reduced soil electrical conductivity by 32.7% on average, increased organic matter by 15.9%, and enhanced the activities of urease, sucrase, and catalase by 142.3%, 46.4%, and 54.6%, respectively. (3) There were growth stage differences between soil physicochemical properties and biological activities: organic matter was the core for driving factor at the cotton seedling stage (significantly positively correlated with microbial biomass carbon and nitrogen, and enzyme activities, P<0.05); the inhibitory effect of electrical conductivity was significantly enhanced at the harvest stage (correlation coefficients -0.5--0.9). (4) The IFI under 100% incorporation treatment of Vicia villosa Roth was the highest (0.76), with a cotton yield of 5 263.6 kg·hm-2 and a net income of 21 477.0 yuan/hm2, an increase of 50.0% compared with CK; although 100% incorporation of Lolium perenne L. increased soil carbon storage, it significantly inhibited cotton growth, and the net income decreased by 24.0% compared with CK.【Conclusion】100% incorporation of Vicia villosa Roth was the optimal improvement model for slightly salinized cotton fields in northern Shandong, which could achieve the coordinated improvement of ecological and economic benefits. The matching mechanism between catch crop functional characteristics and incorporation ratio under this model provided a reference for the improvement of similar salinized farmland.

Key words: cover crop, incorporation ratio, salinized cotton fields, microbial biomass carbon and nitrogen, enzyme activity, soil fertility, economic benefit

Table 1

Above-ground biomass and nutrient accumulation of different cover crops before incorporation"

填闲作物
种类
Cover crop
specie
鲜重
Fresh
weight
(kg·hm-2)
含水量
Moisture content
(%)
干重
Dry
weight
(kg·hm-2)
养分含量Nutrient content (%) 养分累积量Nutrient accumulation (kg·hm-2) C/N
氮干基
N dry-mass basis
磷干基
P dry-mass basis
钾干基
K dry-mass basis
碳干基
C dry-mass basis
TN TP TK TC
Ory 20431.1c 81.5b 3768.1c 1.5b 0.5a 1.8a 47.6b 57.0b 19.3a 69.4c 1794.6b 31.5b
Vic 26068.7b 84.0a 4181.4b 1.7a 0.4a 1.9a 45.4b 70.4a 18.6a 78.5b 1898.9b 27.0c
Lol 31951.4a 81.0b 6058.0a 1.1c 0.4a 1.7b 51.7a 68.1a 25.3a 105.7a 3129.9a 46.0a

Fig. 1

The effects of cover crop species, incorporation ratios, and their interaction on soil available potassium and electrical conductivity at the cotton seedling stage (a) Effects of different cover crops on AK and EC; (b) Effects of different incorporation ratios treatments on AK and EC; (c) Effects of the interaction between different cover crops and incorporation ratios on AK; (d) Effects of the interaction between cover crop species and incorporation ratios on EC. The same as Fig.2. CK: Bare fallow control; Error bars represent standard errors; * Indicates significant differences between treatments (P<0.05, LSD test); ** Indicates extremely significant differences between treatments (P<0.01, LSD test). The same as below"

Fig. 2

Effects of cover crop species, incorporation ratios, and their interaction on soil available potassium and electrical conductivity at the cotton harvest stage"

Fig. 3

Effects of cover crop species, incorporation ratios, and their interaction on soil alkali-hydrolyzable nitrogen and available phosphorus at the cotton seedling stage (a) The impact of different cover crops on AN and AP; (b) The impact of different incorporation ratios on AN and AP; (c) The impact of the interaction between cover crops and incorporation ratios on AN; (d) The impact of the interaction between cover crops and incorporation ratios on AP. The same as Fig.4"

Fig. 4

Effects of cover crop species, incorporation ratios, and their interaction on soil alkali-hydrolyzable nitrogen and available phosphorus at the cotton harvest stage"

Fig. 5

Effects of cover crop species, incorporation ratios, and their interaction on soil pH and organic matter at the cotton seedling stage (a) The impact of different cover crops on pH and SOM; (b) The effect of different incorporation ratios on pH and SOM; (c) The impact of the interaction between cover crops and incorporation ratios on pH; (d) The impact of the interaction between cover crops and incorporation ratios on SOM. The same as Fig.6"

Fig. 6

Effects of cover crop species, incorporation ratios, and their interaction on soil pH and organic matter at the cotton harvest stage"

Table 2

ANOVA for soil basic physicochemical properties as affected by cover crop species and incorporation ratio"

棉花生育时期
Cotton growth period
方差来源
Source of variation
df AP AK AN SOM EC pH
F P F P F P F P F P F P
苗期
Seedling stage
填闲作物Cover crop 3 47.85 0.00 9.89 0.00 12.04 0.00 24.55 0.00 14.85 0.00 1.06 0.36
翻压比例Incorporation ratio 4 15.37 0.00 3.98 0.02 13.17 0.00 7.85 0.00 1.71 0.19 0.62 0.61
填闲作物×翻压比例Cover crop×Incorporation ratio 12 2.72 0.04 0.35 0.90 1.10 0.39 2.61 0.04 1.48 0.23 0.73 0.63
收获期
Harvest stage
填闲作物Cover crop 3 3.82 0.04 18.40 0.00 38.39 0.00 3.07 0.07 6.35 0.01 3.74 0.04
翻压比例Incorporation ratio 4 16.59 0.00 18.27 0.00 8.54 0.00 14.00 0.00 70.10 0.00 3.52 0.03
填闲作物×翻压比例Cover crop×Incorporation ratio 12 1.89 0.12 5.16 0.00 0.83 0.56 3.42 0.01 2.37 0.06 0.55 0.77

Fig. 7

Effects of different treatments on soil microbial biomass carbon and microbial biomass nitrogen throughout the cotton growing season Different letters indicate significant differences among treatments at the same growth stage (P<0.05). The same as below"

Fig. 8

Effects of different treatments on soil urease (URE), invertase (INV), and catalase (CAT) activities throughout the cotton growing season"

Fig. 9

Correlation analysis of soil physicochemical properties and microbial biomass carbon and nitrogen, enzyme activity (a) Cotton seedling stage; (b) Cotton harvest stage. MBC: Microbial biomass carbon; MBN: Microbial biomass nitrogen; INV: Invertase; CAT: Catalase; URE: Urease. The same as below"

Fig. 10

Principal component analysis of soil physicochemical properties and microbial biomass carbon, nitrogen, and enzyme activity"

Table 3

Comparison of comprehensive soil fertility index (IFI) among treatments during the harvest period"

处理Treatment IFI
Ory100% 0.71b
Ory70% 0.59c
Ory30% 0.51d
Ory0 0.43ef
Vic100% 0.76a
Vic70% 0.61c
Vic30% 0.53d
Vic0 0.45ef
Lol100% 0.73ab
Lol70% 0.62c
Lol30% 0.53d
Lol0 0.46e
CK 0.41f

Table 4

Effects of different fallow crops and utilization methods on economic benefits of cotton fields"

处理
Treatment
牧草年收入
Annual forage revenue (yuan/hm2)
棉花产量
Cotton yield(kg·hm-2)
棉花年收入
Annual cotton revenue (yuan/hm2)
周年总收入
Annual gross income (yuan/hm2)
周年总投入
Annual total cost (yuan/hm2)
周年净收益
Annual net benefit (¥/hm2)
Ory100% 0.00 5063.67ab 37977.50ab 37977.50cd 18000 19977.50cd
Ory70% 1838.80 4923.40bc 36925.50bc 38764.30bc 17700 21064.30bc
Ory30% 4290.53 4511.18de 33833.88de 38124.41bcd 17400 20724.41c
Ory0 6129.33 4297.07ef 32228.00ef 38357.33bc 17100 21257.33bc
Vic100% 0.00 5263.60a 39477.00a 39477.00bc 18000 21477.00bc
Vic70% 2346.19 4677.50cd 35081.25cd 37427.44cd 17700 19727.44cd
Vic30% 5474.43 4491.55de 33686.63de 39161.06bc 17400 21761.06bc
Vic0 7820.62 4511.75de 33838.13de 41658.74a 17100 24558.74a
Lol100% 0.00 3849.90g 28874.25g 28874.25f 18000 10874.25f
Lol70% 2875.62 4406.05def 33045.38def 35921.00d 17700 18221.00d
Lol30% 6709.78 4123.25fg 30924.38fg 37634.16cd 17400 20234.16cd
Lol0 9585.41 4102.85fg 30771.38fg 40356.78ab 17100 23256.78ab
CK 0.00 4188.50f 31413.75f 31413.75e 17100 14313.75e
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