Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (9): 1869-1886.doi: 10.3864/j.issn.0578-1752.2026.09.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Effects of Nitrogen Reduction and Potassium Enhancement Fertilization Mode on Photosynthetic Fluorescence Characteristics and Photosynthetic Product Accumulation of Maize in Ningxia Yellow Irrigation Area

CAI QiaoHong(), ZHOU Tian, SU Ming, HONG ZiQiang, LI FanGuo, LI Tong, WANG Hua, KANG JianHong(), WU HongLiang()   

  1. College of Agriculture, Ningxia University, Yinchuan 750021
  • Received:2025-09-23 Accepted:2026-03-19 Online:2026-05-01 Published:2026-05-06
  • Contact: KANG JianHong, WU HongLiang

Abstract:

【Objective】Focusing on the effects of nitrogen reduction and potassium increase fertilization mode on photosynthetic fluorescence characteristics and dry matter accumulation of maize, this paper aimed to elucidate its internal physiological mechanism and provide the theoretical support for the precise fertilization management and green high-yield cultivation of maize nitrogen and potassium.【Method】Field experiments were carried out in Pingjibao Farm, Ningxia from 2023 to 2024, using maize variety Xianyu 1321 as experimental material. The experiment adopted the crack zone design, and the main area was set to different nitrogen application rates, with a total of two levels, namely conventional nitrogen application of 450 kg·hm-2 (N0) and nitrogen reduction of 30% (N1, 315 kg·hm-2). The secondary area was 4 gradients with different potassium application rates, namely conventional potassium application of 45 kg·hm-2 (K0), potassium increase of 50% (K1, 68 kg·hm-2), potassium increase of 100% (K2, 90 kg·hm-2), and potassium increase of 150% (K3, 113 kg·hm-2). The photosynthetic fluorescence parameters, dry matter accumulation and transport and yield components of maize were systematically measured, and the synergistic effects of nitrogen reduction and potassium increase were comprehensively analyzed.【Result】Photosynthetic and fluorescence parameters of maize showed a trend of increasing first and then decreasing with growth period, and N1K2 treatment performed the best. Data from a two-year experiment at tasseling stage showed that net photosynthetic rate (Pn) under N1K2 treatment was increased by 17.53%, 6.74% and 8.16% compared with N1K0, N1K1 and N1K3 treatments, respectively. Transpiration rate (Tr) was significantly increased by 11.57% and 10.89% compared with N1K0 and N1K3 treatments, with no significant difference from N1K1, indicating that appropriate potassium application could effectively improve leaf transpiration efficiency. Maximum photochemical efficiency of PSII (Fv/Fm) was increased by 4.93%-12.71% compared with N1K0, N1K1 and N1K3 treatments. Performance index on absorption basis (PI) was increased by 2.53%-7.81% compared with N1K0, N1K1 and N1K3 treatments. Dry matter accumulation and transport analysis revealed that the N1K2 treatment significantly enhanced pre-harvest dry matter transport to grains, with pre-harvest dry matter accumulation, transport rate, and grain contribution rate increasing by 61.90%, 21.14%, and 27.73% compared with N0K0 treatment, respectively. Yield analysis showed that with production followed a pattern of initial increase followed by decline with nitrogen reduction and potassium increase, achieving peak yields of 18 586.39 and 19 279.5 kg·hm-2 over two years under the N1K2 treatment. Correlation analysis and principal component analysis further confirmed the significant positive correlation between photosynthetic fluorescence parameters and yield under the nitrogen-reduction and potassium-increase pattern.【Conclusion】Reducing nitrogen by 30% and increasing potassium by 100% was the optimal fertilization mode for maize production in the Yellow River diversion irrigation area of Ningxia. N1K2 treatment significantly improved leaf photosynthetic performance and photochemical efficiency, promoted the efficient transport of post-flowering photosynthetic products to grains, increased dry matter accumulation, significantly improved nitrogen fertilizer utilization rate for ensuring high yield of maize, realized the synergistic optimization of photosynthetic efficiency and nutrient use efficiency, and provided a reliable fertilization basis for local maize green production.

Key words: nitrogen reduction and potassium increase, photosynthetic fluorescence properties, accumulation of photosynthetic products, yield, maize

Table 1

Basic physical and chemical properties of 0-20 cm soil in the experimental site from 2023 to 2024"

年份
Year
pH 全氮
Total N (g·kg -1)
全磷
Total P (g·kg -1)
速效钾
Available K (mg·kg -1)
碱解氮
Available N (mg·kg -1)
有机质
Organic matte (g·kg -1)
2023 7.72 0.62 0.64 81.36 41.14 12.18
2024 7.81 0.54 0.55 80.09 45.06 11.83

Fig. 1

Daily average temperature and rainfall distribution in the test area from 2023 to 2024"

Table 2

Nitrogen, phosphorus and potassium fertilizer application rates at different growth stages of maize"

生育时期
Growth period
氮肥处理
Nitrogen fertilizer treatment (kg·hm-2)
钾肥处理
Potassium fertilizer treatment
(kg·hm-2)
磷肥
P2O5
(kg·hm-2)
总施肥量
Total fertilizer application rate
450 (N0) 315 (N1) 45 (K1) 68 (K2) 90 (K3) 113 (K3) 138
苗期 V3 45.0 31.5 13.6 18.0 9.0 22.6 27.6
拔节期 V6 135.0 94.5 18.0 27.2 36.0 45.2 55.2
大喇叭口期 V12 135.0 94.5 13.5 20.4 27.0 33.9 41.4
抽雄期 VT 45.0 31.5 4.5 6.8 9.0 11.3 13.8
灌浆期 R3 90.0 63.0 0 0 0 0 0

Fig. 2

Dynamic changes in net photosynthetic rate of corn under different treatments N0: Conventional nitrogen application (450 kg·hm-2); N1: Nitrogen application reduced by 30% (315 kg·hm-2); K0: Conventional potassium application (45 kg·hm-2); K1: Potassium application increased by 50% (68 kg·hm-2); K2: Potassium application increased by 100% (90 kg·hm-2); K3: Potassium application increased by 150% (113 kg·hm-2); V6: Jointing stage; V12: Large trumpet stage; VT: Tasseling stage; R3: Grain filling stage; R6: Maturity stage. The lowercase letter in the same row of data indicates that there is a significant difference at the 0.05 significance level. The same as below"

Fig. 3

Dynamic changes in corn transpiration rate under different treatments"

Fig. 4

Dynamic changes in stomatal conductance of corn leaves under different treatments"

Fig. 5

Dynamic changes in intercellular carbon dioxide concentration in corn leaves under different treatments"

Fig. 6

Dynamic changes in PSII maximum photochemical efficiency of maize under different treatments"

Fig. 7

Dynamic changes in PSII potential activity of maize under different treatments"

Fig. 8

Dynamic changes in the comprehensive fluorescence performance index of corn under different treatments"

Fig. 9

Dynamic changes in the light energy absorbed per reaction center in corn under different treatments"

Fig. 10

Dynamic variation of dry matter accumulation in maize under synergistic regulation of nitrogen reduction and potassium increase"

Fig. 11

Dynamic changes of nitrogen reduction and potassium increase on dry matter allocation of maize"

Table 3

Changes of nitrogen reduction and potassium increase on dry matter transport before and after flowering of maize"

年份
Year
施氮处理
Nitrogen application treatment
施钾处理
Potassium application treatment
花前 Preanthesis 花后 Postanthesis
积累量
DMR (kg·hm-2)
转运率
DMRE (%)
贡献率
DMRCG (%)
积累量
DMA (kg·hm-2)
贡献率
DMAC (%)
2023 N0 K0 2269.50a 18.01a 12.71ab 15005.10c 87.73ab
K1 2481.90a 21.08a 12.29ab 16978.80b 87.71ab
K2 2505.60a 18.31a 14.68a 19082.10a 89.42a
K3 2305.80a 19.87a 10.58a 14093.70d 85.53b
N1 K0 2395.80b 20.53ab 10.99b 18992.10c 89.02a
K1 2430.30b 18.88b 10.50b 18669.60c 85.56b
K2 3318.90a 22.58a 14.74a 22617.60a 90.67a
K3 2370.00b 19.68ab 9.33b 20388.30b 89.50a
2024 N0 K0 3681.00b 25.98b 16.46b 14214.00b 74.72c
K1 3805.20b 25.35b 16.79b 17490.45a 78.83b
K2 5005.80a 33.44a 21.17a 17876.10a 83.54a
K3 3488.40b 32.10b 15.65b 17832.45a 84.35a
N1 K0 3096.00c 23.67c 14.17c 17790.00c 85.83a
K1 5877.60a 30.44ab 23.33a 18175.35bc 75.90c
K2 6314.85a 30.71a 22.52a 20404.95a 77.48c
K3 4757.70b 27.73b 19.36b 18574.95b 81.05b
ANOVA 年份Year (Y) ** ** ** ** **
施氮量Nitrogen application (N) ** ** ** ** NS
施钾量Potassium application (K) ** ** ** ** **
N×K ** * ** ** NS
Y×N ** NS ** ** NS
Y×K ** ** ** ** **
Y×N×K ** NS * ** *

Table 4

Effects of nitrogen reduction and potassium reduction and potassium reduction on maize yield from 2023 to 2024"

年份
Year
施氮处理
Nitrogen application treatment
施钾处理
Potassium application treatment
穗数
Ear number
(ear/hm2)
穗粒数
Grain number
per ear
百粒重
100-kernel
Weight (g)
产量
Yield
(kg·hm-2)
2023 N0 K0 83031.12c 484.88a 35.73b 15650.37b
K1 84562.48b 501.68a 36.82ab 16210.02b
K2 87624.64a 510.70a 37.84a 17597.08a
K3 82447.48c 483.53a 35.29b 15888.59b
N1 K0 86746.42b 507.41c 36.42c 15261.12b
K1 88133.91b 536.22a 38.76b 18203.54a
K2 90941.46a 540.98a 40.27a 18586.39a
K3 88018.23b 519.26b 38.53b 18161.88a
2024 N0 K0 85702.73c 666.79a 28.21b 16352.58b
K1 87311.51b 688.01a 29.07a 16488.74b
K2 90497.75a 701.38a 29.15a 17305.46a
K3 87151.67b 672.43a 28.06a 16785.16b
N1 K0 83630.90d 652.80c 27.90c 14895.10b
K1 88008.11c 715.66ab 32.02a 18392.79a
K2 92543.93a 731.15a 30.12b 19279.56a
K3 90261.99b 703.31b 29.16b 18384.68a
ANOVA 年份Year (Y) ** ** ** **
施氮量Nitrogen application (N) ** ** ** **
施钾量Potassium application (K) ** ** ** **
N×K ** * ** **
Y×N ** NS * NS
Y×K ** NS * NS
Y×N×K * NS * NS

Fig. 12

Correlation analysis and principal component analysis of photosynthetic fluorescence parameters and yield of maize by reducing nitrogen and increasing potassium"

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