Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (20): 4012-4020.doi: 10.3864/j.issn.0578-1752.2017.20.015


Effects of Different Carriers on Near Infrared Quantitative Model of Vitamin E in Premix

WANG Yanni1, CHEN Yueyan2, ZHA Shanshan3, WANG Shi1, XIAO Zhiming1, LI Shouxue1, FAN Xia1   

  1. 1Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081; 2Animal Feed Quality and safety of Veterinary Testing Centre in Liaoning Province, Shenyang 110016; 3PerkinElmer, Beijing 100015
  • Received:2017-04-25 Online:2017-10-16 Published:2017-10-16

Abstract: 【Objective】Vitamin E is an important feed additive, the accurate determination of its content is of great significance for the preparation of dietary. In this paper, the rapid quantitative method of vitamin E in different carrier premixed feeds was studied. The aim of this paper was to describe the applicability of near infrared spectroscopy to different carrier premixed feeds, and to establish a near-infrared rapid measuring method for vitamin content in premixed feed.【Method】Commercial vitamin E powder was collected and mixed with the silica, defatted rice bran and stone powder. The premix of vitamin E in the concentration range of 5-250 IU·g-1was prepared by blending and collected its near-infrared spectra. This study compared the effects of different scanning times and resolution on the quality of the near infrared spectroscopy, compared the characteristic peak of vitamin E in premix of different carrier and different spectral preprocessing methods quantitative accuracy of PLS model and the difference of the model was analyzed from the point of view of VIP Score in PLS model. 【Result】 The results showed that 16 cm-1 resolution and 32 scans were suitable for spectral acquisition. There were significant differences in NIR spectra of premix with different carriers. The vitamin E absorption peaks of silica, defatted rice bran and stone powder as the carriers of the premix were 8 700-8 200, 7 100- 7 450, 6 020-5 550, 4 440-4 200, 5 210-4 760, 8 550-8 050, 7 410, 5 950-5 200, 4 400-4 050, 4 750-4 600 cm-1 and 8 550-8 050,   7 250, 5 950-5 200, 4 600-4 000, and 4 800-4 700 cm-1, respectively. The optimal spectral pretreatment methods for the near-infrared quantitative models were SNV+1stDeriv(2,15), MSC+1stDeriv(2,21)and SNV+2stDeriv(2,15), respectively, the calibration (Rc2)were 0.988, 0.949 and 0.941, respectively, the standard error of calibration (SEP)were 8.86, 10.36 and 23.37, respectively, the validation (Rp2) are 0.989, 0.980 and 0.921 respectively, the standard error of prediction(SEP)were 8.86, 10.36 and 23.37 respectively, and ratio of performance to standard deviation(RPD)were 8.10, 6.90 and 3.07 respectively. The VIP Scores value of PLS model with three different carrier diluents were extracted, and the contribution of the VIP Score values of silica were significant and that associated with vitamin E characteristic peaks was the highest, then was the skim rice bran and followed by stone powder.【Conclusion】Near-infrared spectroscopy for the analysis of vitamin E premixed feed, with the silica and skim rice bran as the carriers of the pre-mixed feed is better, with stone powder as the carrier of premixed feed, applicability of the measuring method is poor.

Key words: carrier, vitamin E, premix feed, quantitative model

[1]    杨凤. 动物营养学. 北京: 中国农业出版社, 1999.
YANG F. Animal Nutrition. Beijing: China Agricultural Press, 1999. (in Chinese)
[2]    国务院令2011年第609号. 饲料和饲料添加剂管理条例.
State Council Decree No. 609 of 2011. Regulations on the Administration of Feed and Feed Additives. (in Chinese)
[3]    龙伊, 朱晓莉, 邓江玲. 如何正确选择添加剂预混料的载体和稀释剂. 草食家畜, 2009(4): 55-56.
LONG Y, ZHU X L, DENG J L. How to properly select the additive premix for the carrier and diluent. Herbivorous Livestock, 2009(4): 55-56. (in Chinese)
[4]    任一平, 陈恒武, 陈青俊, 包敛斌, 黄百芬, 何巧红. 柱后光化学衍生荧光检测高效液相色谱法测定食品中的维生素B1. 分析化学, 2000, 28(5): 554-558.
REN Y P, CHEN H W, CHEN Q J, BAO L B, HUANG B F, HE Q H. Determination of thiamine in food by high performance liquid chromatography with post-column photochemical derivatization and flouresence detection. Chinese Journal of Analytical Chemistry, 2000, 28(5): 554-558. (in Chinese)
[5]    王晓峰. 高效液相色谱法测定预混料中维生素的研究. 价值工程, 2015(7): 284-285.
WANG X F. On the thesis research of vitamin in premixed fodder by HPLC. Value Engineering, 2015(7): 284-285.
[6]    ERIK C, ELS V, STEFAAN D S. Determination of α-tocopherol by reversed-phase HPLC in feed and animal-derived foods without saponification. The Science of Food and Agriculture, 2016, 96: 522-529.
[7]    梁琳, 王英. HPLC 法同步测定维生素预混合饲料中5种B族维生素. 中国饲料, 2016(1): 21-23, 38.
LIANG L, WANG Y. Simultaneous determination of 5 B vitamins in vitamin premix feed by HPLC. Chinese Feed, 2016(1): 21-23, 38. (in Chinese)
[8]    杨发树, 赵艳, 刘耀敏, 张凤枰. 高效液相色谱法测定预混合饲料中的维生素K3. 饲料研究, 2016(2): 51-53.
YANG S F, ZHAO Y, LIU Y Q, ZHANG F P. Determination of vitamin K3 in premix feed by high performance liquid chromatography. Feed Research, 2016(2): 51-53. (in Chinese)
[9]    周兴藩. 不同非常规蛋白饲料原料成分特性的表征研究[D]. 北京: 中国农业大学工学院, 2014.
ZHOU X F. Characterization of different unconventional protein fennd materials[D]. Beijing: College of Engineering, China Agricultural University, 2014. (in Chinese)
[10]   陆婉珍. 现代近红外光谱分析技术. 北京: 中国石油化工出版社, 2001.
LU W Z. Modern Near Infrared Spectroscopy Analytical Technology. Beijing: China Petrochemical press, 2001. (in Chinese)
[11]   褚小立. 化学计量学方法与分子光谱分析技术. 化学工业出版社, 2011.
CHU X L. Chemometrics and Molecular Spectroscopy. Chemical Industry Press, 2011. (in Chinese)
[12]   INMACULADA G, JOSÉ M G C M B R, M. MILAGROS D Z. Near infrared spectroscopy (NIRS) reflectance technology for determination of tocopherols in animal feeds. Analytica Chimica Acta, 2006, 558: 132-136.
[13]   王文杰, 穆淑兰, 刘兰兰, 任鹏. 近红外光谱法检测预混料中的维生素A. 天津畜牧兽医, 1998, 15(2): 12-14.
WANG W J, MU S L, LIU L L, REN P. Determination of vitamin A in premix by near infrared spectroscopy. Tianjin Animal Husbandry and Veterinary, 1998, 15(2): 12-14. (in Chinese)
[14]   王文杰, 穆淑兰, 刘兰兰, 任鹏. 预混料中维生素D3、K3的近红外光谱分析. 中国饲料, 1998(15): 23-24.
WANG W J, MU S L, LIU L L, REN P. Analysis of vitamin D3 and K3 in premix by near infrared spectroscopy. China Feed, 1998(15): 23-24. (in Chinese)
[15]   李秋玫, 饶宏英, 阮静. 利用近红外漫反射(NIR)技术快速检测多维饲料中维生素E的含量. 中国饲料, 2005, 15(4): 36-37.
LI Q M, RAO H Y, RUAN J. Rapid detection of vitamin E in multi dimensional feed by near infrared diffuse reflectance spectroscopy (NIR). China Feed, 2005, 15(4): 36-37. (in Chinese)
[16]   石冬冬, 刘志英, 常淑平, 马书宇, 刘庆生. 利用近红外图谱技术同时检测预混料中多种维生素的研究. 粮食与饲料工业, 2015, 12(8): 61-65.
SHI D D, LIU Z Y, CHANG S Pg, MA S Y, LIU Q S. Determination of several vitamins contents in premix feed by near-infrared spectroscopy. Cereal and Feed Industry, 2015, 12(8): 61-65. (in Chinese)
[17]   DOUGLAS A S, F. JANES H, STANLEY R. C. Principles of Instrumental Analysis. Thomson Brooks, Cole, 2007.
[18]   耿姝. 适宜中药材体系的近红外分析方法影响因素研究[D]. 杭州: 浙江大学药学院, 2016.
GENG S. Studies on influential factors of near-infrared spectroscopy for chinese medicinals system[D]. Hangzhou: College of Pharmaceutial Science, Zhejiang University, 2016. (in Chinese)
[19]   XUE J J, YANG Z L, HAN L J, CHEN L J. Study of the influence of NIRS acquisition parameters on the spectral repeatability for on-line measurement of crop straw fuel properties. Fuel, 2014, 117: 1027-1033.
[20]   吴静姝, 刘翠玲, 邢素霞, 孙梅. 光谱分辨率的选取对食用油近红外模型性能影响分析. 北京工商大学学报, 2012, 30(1): 66-68.
WU J S, LIU C L, XING S X, SUN M. Research on NIR model of edible oil impacted by spectral resolution determination. Journal of Beijing Technology and Business University, 2012, 30(1): 66-68. (in Chinese)
[21]   段焰青, 杨涛, 孔祥勇, 汤丹瑜, 李青青. 样品粒度和光谱分辨率对烟草烟碱NIR预测模型的影响. 云南大学学报(自然科学版), 2006, 28(4): 340-344.
DUAN Y Q, YANG T, KONG X Y, TANG D Y, LI Q Q. Effect of particle size and spectral resolution on nicotine NIR prediction model of tobacco. Journal of Yunnan University, 2006, 28 (4): 340-344. (in Chinese)
[22]   闵顺耕, 李宁, 张明祥. 近红外光谱分析中异常值的判别与定量模型优化. 光谱学与光谱分析, 2004, 24(10): 1205-1209.
MIN S G, LI N, ZHANG X M. Outlier diagnosis and calibration model optimization for near infrared spectroscopy analysis. Spectroscopy and Spectral Analysis, 2004, 24(10): 1205-1209. (in Chinese)
[23]   刘贤, 韩鲁佳. 近红外漫反射光谱法快速测定秸秆青贮饲料成分含量. 光谱学与光谱分析, 2006, 26(11): 2016-2019.
LIU X, HAN L J. Feasibility of using near-infrared reflectance spectroscopy for the analysis of compositions of straw silage. Spectroscopy and Spectral Analysis, 2006, 26(11): 2016-2019. (in Chinese)
[24]   彭自栋, 申俊峰, 曹卫东, 刘海明, 李金春, 张兆宇. 近红外分析提取蚀变信息及其找矿实践—以甘肃岗岔金矿为例. 中国地质调查, 2015, 2(3): 28-39.
PENG Z D, SHEN J F, CAO W D, LIU H M, LI J C, ZHANG Z Y. Extraction of alteration information by near infrared analysis and its prospecting practice—taking Gansu Gangzha Gold Mine as an example. Geological Survey of China, 2015, 2(3): 28-39. (in Chinese)
[25]   耿韫, 郄冰冰. 近红外光谱特征峰快速鉴别1例假劣药品. 河北医药, 2015, 34(9): 1405-1406.
GENG Y, XI B B. Nearly infrared spectrum characteristic peak rapid identification of 1 case of counterfeit drugs. Hebei Medical Journal, 2015, 34(9): 1405-1406. (in Chinese)
[26]   李秀明, 刘波, 朱克旭, 涂登云. 近红外光谱图特征峰在假药分析中的应用. 中国药事, 2008, 22(8): 558-559.
LI X M, LIU B, ZHU K X, TU D Y. Application of near infrared spectral spectrum characteristic peak in counterfeit drug analysis. Chinese Medicine, 2008, 22(8): 558-559. (in Chinese)
[27]   MARTR B, REMO Bi, ANDREA D. Tracing the origin of extra virgin olive oils by infrared spectroscopy and chemometrics: A case study. Analytica Chimica Acta, 2012, 717: 39-51.
[28]   FAN X, TANG S C, LI G Z, ZHOU X F. Non-invasive detection of protein content in several types of plant feed materials using a hybrid near infrared spectroscopy model. PLoS One, 11(9): 1-12.
[29]   孙德欣, 娜日娜, 宋文静. 添加剂应用载体和稀释剂的必要性. 江西饲料, 2014(4): 11-13.
SUN D X, NA R N, SONG W J. The necessity of application of additives and diluents. Jiangxi Feed, 2014(4): 11-13. (in Chinese)
[30]   蔡娟. 饲料预混料载体与稀释剂的选择. 饲料与添加剂, 2003(9): 20-21.
CAI J. Selection of feed premix carrier and diluent. Feed and Additives, 2003(9): 20-21. (in Chinese)
[1] YAO XingLan,YANG WenZhu,LUO YanZhong,CHEN RuMei,WANG Lei,ZHANG Lan. Acquisition and Characteristic Analysis of Transgenic Maize with phyA2, ZmTMT, and Bar [J]. Scientia Agricultura Sinica, 2020, 53(24): 4982-4991.
[2] ZHAO Jiao, ZHOU Zhao-Hong, LIANG Xiao-Fang, MAO Xiang-Bing, CHEN Dai-Wen, YU Bing. Effects of GSPs and VE on Growth Performance, Serum Redox Status and Hepatic Oxidative Damage in Piglets Under Oxidative Stress [J]. Scientia Agricultura Sinica, 2013, 46(19): 4157-4164.
[3] GUO An-Qiang, WAN Yong-Shan, LIU Feng-Zhen. Cloning and Polymorphism of 2-Methyl-6-Phytyl-1, 4-Benzoquinol Methyltransferase Gene (VTE3) in Arachis [J]. Scientia Agricultura Sinica, 2012, 45(9): 1685-1695.
[4] PENG Bing-Yong, GAO Yu-Peng, WANG Chun-Jiang, ZHENG Hai-Zu, HUANG Fu-Yi-Fan, JI Jian-Xin. Modulation of Dietry Vitamin E on Economic Characterization of Immunosupressed Laying Hens [J]. Scientia Agricultura Sinica, 2011, 44(24): 5083-5091.
[5] LEI Hong-tao,PANG Jie,HE Li-ping,YANG Jin-yi,SUN Yuan-ming,QIN Yi-lan,LIANG Ming-biao
. Identification of Hapten-Carrier Protein Conjugate by Three Electrophoresis Methods
[J]. Scientia Agricultura Sinica, 2010, 43(3): 565-570 .
[6] XU Miao-yun,ZHOU Jian,ZHANG Lan,FAN Yun-liu,WANG Lei
. Cloning of Glycine Max 2-Methyl- 6-Phytyl-1,4-Benzoquinol Methyltransferase (Gm VTE3) Gene and Engineering Vitamin E Content in Seed of Transgenic Tobacoo
[J]. Scientia Agricultura Sinica, 2010, 43(10): 1994-1999 .
Full text



No Suggested Reading articles found!