北方寒旱区白菜型冬油菜抗寒性与抗旱性评价及其关系

doi:10.3864/j.issn.0578-1752.2015.18.018

Abstract

Abstract: 【Objective】An experiment was conducted to study the cold and drought resistance and its relationship of winter rapeseed. The aim of this study was to provide valuable methods and theories for improvement and overall evaluation of cold and drought resistance of winter rapeseed in north China.【Method】Six rapeseed varieties which have different cold resistance abilities were used to simulate low temperature and drought stress which were implemented by natural drop in temperature (15℃--5℃) and manual water control (days of drought stress were 4 d, 7 d, and 10 d). Changes in morphology, physiology, biochemistry and growth of the winter rapeseed were analyzed. Subordinate function coefficients, fuzzy clustering, correlation analysis and principal component analysis were used to evaluate the cold and drought tolerance comprehensively.【Result】The results showed that the over wintering rate of the 6 different winter rapeseed varieties varied from 20.1% to 94.7%. Morphological characteristics of botany of the rapeseed variety with stronger cold-resistance level was prostrate at seedling stage, the growing point was sagging and below the surface, the leaves color presented dark green and bristles much-ness. The physiological and biochemical indexes changed significantly after cold stress, relative conductivity and content of MDA increased under cold stress, and the increase rate of which became slow with the increase of levels of cold-resistance. But the activities of SOD, POD, CAT and content of SP, SS, Pro were overall ascendant, the variation trend of which became larger with the increase of level of cold-resistance. Under drought stress, the relative conductivity and the content of MDA increased, and the content of chlorophyll and leaf relative water and the ratio of bond water and free water decreased, the length of seedling, fresh and dry weight of leaves and roots decreased. They have a strong capacity of soil water retention, and the wilting coefficient was small, and the rate of range became slow with the strong level of drought-resistance of winter rapeseed varieties. The order of cold-resistance levels of the six winter rapeseed varieties is Longyou 7＞ Longyou 6＞Longyou 9＞Yanyou 2＞Tianyou 2＞Vision, and the drought-resistance levels of the six winter rapeseed varieties is Longyou 6＞Longyou 7＞Longyou 9＞Yanyou 2＞Vision＞Tianyou 2 by integrated evaluation of the main components .【Conclusion】In north China, cold and dry exist side by side and different varieties have significant different abilities of cold and drought resistance, which resulted in cross adaptation of winter rapeseed, so the winter rapeseed varieties with strong cold resistance planted in north China also have strong drought resistance.

Key words: cold and arid region, winter rapeseed (Brassica campestris L.), cold resistance, drought resistance

LIU Hai-qing, SUN Wan-cang, LIU Zi-gang, WU Jun-yan, QIAN Wu, WANG Zhi-jiang, GUO Ren-di, MA Li, HOU Xian-fei, LIU Lin-bo. Evaluation of Drought Resistance and Cold Resistance and Research of Their Relationship at Seedling Stage of Winter Rapeseed (Brassica campestris L.) in Cold and Arid Regions in North China[J].Scientia Agricultura Sinica, 2015, 48(18): 3743-3756.

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Evaluation of Drought Resistance and Cold Resistance and Research of Their Relationship at Seedling Stage of Winter Rapeseed (Brassica campestris L.) in Cold and Arid Regions in North China

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