光照时间和环境温度对种鹅繁殖系统及相关激素mRNA表达、分泌的影响

doi:10.3864/j.issn.0578-1752.2015.13.015

Abstract

Abstract: 【Objective】The experiment was conducted to study the effects of illumination time and ambient temperature treatments on reproductive system and gene expression and secretion of hormone in breeding Yangzhou geese, so that the functional characteristics of illumination time and ambient temperature could be preliminarily discovered. 【Method】 The geese of 200-day-old and receiving gradually decreased natural sunlight were selected as experimental animals. At the beginning of experiment, the sunlight time was 9.7 h a day, and the ambient temperature was about 5℃. Treatments were assigned in a completely randomized design with factorial arrangement (2 × 3) including 2 levels of ambient temperature (0-5℃, 25-30℃) and 3 levels of illumination time (8 h, 12 h, 16 h). The illumination increased and decreased based on the natural day. In this study 120 adult female geese were randomly assigned to 6 groups with 20 birds each. Feed and water were freely available. On day 30, 5 geese of each treatment were taken randomly to collect 3 mL blood sample from wing venous for determination of related hormone indexes. At the same time, 3 geese of each treatment were got randomly to be slaughtered and hypothalamus and pituitary were collected quick-freezed and then conserved in ultra-low-temperature storage for determination of related gene expression. The reproductive system was separated to determine weight or length, simultaneously oviduct magnums were taken for tissue slice. 【Result】Serum concentrations of luteinizing hormone (LH) in 16 h illumination treatments were significantly more than those in 8 h and 12 h illumination treatments (P＜0.05). Serum concentrations of prolactin (PRL) in 16 h illumination treatments were significantly more than those in 12 h illumination treatments (P＜0.05). Serum concentrations of PRL in high temperature treatments (25-30℃) were significantly higher than those in low temperature treatments (0-5℃) (P＜0.05). There were significant interactions on serum concentrations of LH, PRL and estradiol (E₂) between illumination time and ambient temperature (P＜0.05). The ovary index and follicle index of 12 h illumination treatments were significantly more than those of 16 h illumination treatments (P＜0.05). The follicle number of 8 h illumination treatments was significantly more than that of 16 h illumination treatments (P＜0.05). The oviduct length of 12 h illumination treatments was significantly more than those of 8 h and 16 h illumination treatments (P＜0.05), and the oviduct index of high temperature treatments was significantly higher than that of low temperature treatments (P＜0.05). There were no significant effects on ovary index, follicle index, follicle number, oviduct index and oviduct length under different ambient temperatures (P＞0.05). The oviduct magnum of 8 h and 12 h illumination treatments and the high temperature treatments showed scarlet. In addition, the pseudostratified columnar epithelium and lamina propria were thicker, morever their rugas were obvious, and the secretory glands were rich. However, the oviduct magnum of 16 h illumination treatments and the low temperature treatments showed dull red. Their pseudostratified columnar epithelium and lamina propria were obviously thinner, furthermore rugas were fuzzy, and the secretory glands were less. Relative expression quantity of PRL gene of 16 h illumination treatments was significantly higher than that of 8 h and 12 h illumination treatments (P＜0.05). There were no interactions on reproductive system, mRNA expression and secretion of hormone in breeding geese (P＞0.05). 【Conclusion】Long time illumination of 16 h suppressed growth of ovarian and follicle, and illumination of 12 h increased oviduct length. Long time illumination of 16 h increased expression abundance of PRL gene. Therefore, long illumination time depressed reproductive function, and the optimum illumination time of geese was 12 h. High temperature increased serum concentration of LH and PRL, and the organization structure of breeding geese were affected by low temperature, so the ambient temperature should be controlled, and the breeding geese need to avoid being raised in the extreme temperature environment.

Key words: illumination, ambient temperature, breeding goose, reproductive hormone, reproductive system

YANG Hai-ming, JU Xiao-jun, WANG Zhi-yue, DING Jia-tong, WANG Xin-xi, CHEN Yong-hua. Effects of Illumination Time and Ambient Temperature on Reproductive System and Gene Expression and Secretion of Hormone in Breeding Geese[J].Scientia Agricultura Sinica, 2015, 48(13): 2635-2644.

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Effects of Illumination Time and Ambient Temperature on Reproductive System and Gene Expression and Secretion of Hormone in Breeding Geese

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