中华蜜蜂二倍体雄蜂人工培育及形态测定

doi:10.3864/j.issn.0578-1752.2014.22.019

Abstract

Abstract: 【Objective】 The objective of this experiment is to study artificial rearing and determination of morphological index of diploid drones, and to reveal the biological characters of Apis cerana cerana. 【Method】 Queens were firstly reared by the method of larvae double-transferring, and then anaesthetized with CO₂. The colony were fed with sucrose for stimulative feeding. Unfertilized eggs were laid by queen, and they were marked by painting after emergence. Haploid drones of sexual maturity were caught for insemination with their mother queen. The queen was then controlled to lay eggs on an empty frame. Larvae developed from the eggs were transferred to incubator (RH: 95%; T: 35℃) for artificial rearing. Larvae in first 3 days were fed with diet containing 90% royal jelly and 10% ddH₂O. During 4-6 days old, the larvae food was changed to 50% royal jelly, 6% fructose, 6% glucose, 1% yeast extract and 37% ddH₂O. From 7 days old to defecating period the diet is constituted of 43% royal jelly, 9% fructose, 9% glucose, 1% yeast extract and 38% ddH₂O. The cell ploidy of cultivated drones was determined using flow cytometry instrument and morphological index of haploid and diploid drones in colony were measured. 【Result】 The total emerged rate in honeybee of artificial feeding was low (36% in average), in which 26.9% were drones. There were 92% of emerged drones reared in vitro and 82% developed in natural colony drones were diploid examined by the cell ploidy. According to the morphological index investigation, the birth weight and reproductive organs of diploid drones (99.78 and 6.05 mg, respectively) were significantly smaller than that of haploid drones (105.64 and 7.02 mg, respectively), and in other indexs (the fore wing length, the fore wing width, the number of the hooks on the wing, etc) the difference was not significant. 【Conclusion】 After inbreeding, the queens (A. cerana cerana) can produce the diploid drones, and some larvae can reach adult stage. And some morphological differences exist between them and haploid drones.

Key words: Apis cerana cerana; diploid drones, artificial rearing, morphological determination

GAN Hai-yan, LI Shu-yun, ZENG Zhi-jiang, YAN Wei-yu. Artificial Rearing and Morphological Determination of the Diploid Honey Bee in Apis cerana cerana[J].Scientia Agricultura Sinica, 2014, 47(22): 4533-4539.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2014.22.019

https://www.chinaagrisci.com/EN/Y2014/V47/I22/4533

References

[1] 曾志将. 养蜂学. 2版. 北京: 中国农业出版社, 2009.

Zeng Z J. Apiculture. 2nd ed. Beijing: China Agriculture Press, 2009. (in Chinese)

[2] 颜伟玉, 吴晓波, 王子龙, 曾志将. 蜂群中的二倍体雄蜂. 蜜蜂杂志, 2010(12): 10-12.

Yan W Y, Wu X B, Wang Z L, Zeng Z J. The dilploid drones in colony. Journal of Bee, 2010(12): 10-12. (in Chinese)

[3] Cournault L,Aron S. Diploid males, diploid sperm production, and triploid females in the ant Tapinoma erraticum. Naturwissenschaften, 2009, 96: 1393-1400.

[4] Beye M, Hasselmann M, Fondrk M K, Page R E, Omholt S W. The gene csd is the primary signal for sexual development in the honeybee and encodes an SR-type protein. Cell, 2003, 114(4): 419-429.

[5] Woyke J. The hatchability of ‘lethal’ eggs in a two sex-allele fraternity of honeybees. Journal of Apicultural Research, 1962, 1: 6-13.

[6] Woyke J. Drone larvae from fertilized eggs of the honeybee. Journal of Apicultural Research, 1963, 2(1): 19-24.

[7] Woyke J. What happens to diploid drone larvae in a honeybee colony. Journal of Apicultural Research, 1963, 2(2): 73-75.

[8] Woyke J. Rearing and viability of diploid drone larvae. Journal of Apicultural Research, 1963, 2(2): 77-84.

[9] Woyke J. Study on the comparative viability of diploid and haploid larval drone honeybees. Journal of Apicultural Research, 1965, 4(1): 12-16.

[10] Woyke J. Rearing diploid drone larvae in queen cells in a colony. Journal of Apicultural Research, 1965, 4(3): 143-148.

[11] Woyke J. Do honeybees eat diploid drone larvae because they are in worker cells? Journal of Apicultural Research, 1965, 4(2): 65-70.

[12] Woyke J, Knytel A. The chromosome number as proof that drones can arise from fertilized eggs of the honeybee. Journal of Apicultural Research, 1966, 5(3): 149-154.

[13] Woyke J. A method of rearing diploid drones in a honeybee colony. Journal of Apicultural Research, 1969, 8(2): 65-74.

[14] Woyke J. Sex derermination in Apis cerana indica. Journal of Apicultural Research, 1979, 18(2): 122-127.

[15] Woyke J. Evidence and action of cannibalism substance in Apis cerana indica. Journal of Apicultural Research, 1980, 19(1): 6-16.

[16] Hoshiba H, Okada I, Kusanagi A.Thediploid drone of Apis cerana japonica and its chromosomes. Journal of Apicultural Research, 1981,20(3): 143-147.

[17] Polaczek B, Neumann P, Schricker B, Moritz R F A. A new, simple method of rearing diploid drones in the honeybee (Apis mellifera L.). Apidologie, 2000, 31: 525-530.

[18] 李位三, 余林生. 中华蜜蜂二倍体雄蜂的产生及生物学特性. 蜜蜂杂志, 1990(12): 3-6.

Li W S, Yu L S. The generation and biological characteristics of the Apis cerana cerana diploid drones. Journal of Bee, 1990(12): 3-6. (in Chinese)

[19] 余林生, 孟祥金, 陈宏权. 中华蜜蜂产生二倍体雄蜂的生物学研究. 中国养蜂, 2004, 55(6): 7-9.

Yu L S, Meng X J, Chen H Q. Biological research on diploid drones produced by Apis cerana cerana. Apiculture of China, 2004, 55(6): 7- 9. (in Chinese)

[20] 江敬晧. 室内人工饲养蜜蜂幼虫之研究[D]. 台北: 国立台湾大学, 1999.

Jiang J H. Study on indoor artificial feeding of honeybee larvae[D]. Taibei: National Taiwan University, 1999. (in Chinese)

[21] 耿小丽, 魏臻武, 姚喜红, 赵艳. 苜蓿花药培养再生植株染色体倍性检测研究. 草地学报, 2010, 18(5): 714-718.

Geng X L, Wei Z W, Yao X H, Zhao Y. Anther culture of Medicago sativa L. and ploidy detection. Acta Agrestia Sinica, 2010, 18(5): 714-718. (in Chinese)

[22] Dolezel J, Binarova P, Lucretti S. Analysis of nuclear DNA content in plant cells by flow cytometry. Biologia Plantarum, 1989, 31(2): 113-120.

[23] Vandenberg J D, Shimanuki H. Technique for rearing worker honeybees in the laboratory. Journal of Apicultural Research, 1987, 26(2): 90-97.

[24] Peng C Y S, Mussen E. Effects of chlortetracycline of honeybee worker larvae reared in vitro. Journal of Invertebrate Pathology, 1992, 60: 127-133.

[25] 刘光楠, 张飞, 颜伟玉, 吴小波, 曾志将. 蜂王幼虫与工蜂幼虫发育期食物消耗量的研究. 应用昆虫学报, 2011, 48(1): 113-115.

Liu G N, Zhang F, Yan W Y, Wu X B, Zeng Z J. Study on the quantity consuming of food in larvae stage of queen and worker. Chinese Journal of Applied Entomology, 2011, 48(1): 113-115. (in Chinese)

[26] 王倩, 孙亮先, 肖培新, 刘锋, 康明江, 胥保华. 室内人工培育中华蜜蜂幼虫技术研究. 山东农业科学, 2009(11): 113-116.

Wang Q, Sun L X, Xiao P X, Liu F, Kang M J, Xu B H. Study on technology for indoor artificial feeding of Apis cerana cerana larvae. Shandong Agricultural Sciences, 2009(11): 113-116. (in Chinese)

[27] 颜伟玉, 潘珂, 李琳, 曾志将. 培育雄蜂和工蜂所需能量值的研究. 经济动物学报, 2004, 8(3): 146-147.

Yan W Y, Pan K, Li L, Zeng Z J. Studies on the general energy needed for rearing a drone and a worker. Journal of Economic Animal, 2004, 8(3): 146-147. (in Chinese)

[28] Cournault L, Aron S. Rapid determination of sperm number in ant queens by flow cytometry. Insectes Sociaux, 2008, 55: 283-287.

[29] Woyke J. Comparative biometrical investigation on diploid drones of the honeybee Ⅲ. The abdomen, and weight. Journal of Apicultural Research, 1978, 17(4): 206-217.

[30] Herrmann M, Trenzcek T, Fahrenhorst H, Engels W. Characters that differ between diploid and haploid honey bee (Apis mellifera) drones. Genetics and Molecular Research, 2005, 4(4): 624-641.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Artificial Rearing and Morphological Determination of the Diploid Honey Bee in Apis cerana cerana

RichHTML

PDF

Abstract

Cite this article

share this article

References

Related Articles 1

Metrics

Comments

Recommended 0