卵巢摘除对山羊血清GH水平和组织中GHR基因表达的影响

doi:10.3864/j.issn.0578-1752.2014.01.021

Abstract

Abstract: 【Objective】This study was conducted to investigate the effects of ovariectomy on the meat performance, serum GH (growth hormone) levels and the relative expression levels of GHR (growth hormone receptor) mRNA in longissimus and biceps femoris tissues, liver and perirenal fat tissue, and to reveal the reasons of fattening after ovariectomy in female goats.【Method】Forty young hybrid does Kid of Boer Goats (Boer goat ♂ × Guanzhong dairy goat ♀) with similar body weight at about 5 months old were randomly divided into treatment group (n=20) and control group (n=20), the works of insect repellent and epidemic prevention were implemented a month ago before the beginning of the experiment. The goats in the treatment group were ovariectomized at the beginning of the experinment and the goats were not done in control group. Venous blood samples (10 ml) were collected from each goat to separate serum via jugular vein puncture using clean sterile syringes and needles with no additive at 8:00 in the morning on days 0, 10, 20, 30, 40 and 50 during the experiment. Then the serum was separated from blood samples to determine the levels of the growth hormone (GH) with specific ELISA kits (enzyme-linked immunosorbent assay kit) according to the manufacturer’s instruction. The slaughter rate, lean percentage of carcass, loin eye area and proportion of bone and meat were measured after 10 goats (5 goats of each group) were slaughtered on the 50 th day. At the same time, longissimus and biceps femoris tissues, liver and perirenal fat samples were collected immediately after goats were sacrificed, then three biological replicates were collected at each sample, and the samples were rinsed with sterile saline to remove blood clots and wrapped in aluminum foil with good marks, shock-frozen in liquid nitrogen. The mRNA expression levels of GHR gene in liver, longissimus muscle, biceps femoris and perirenal fat were detected with real-time quantitative PCR (qRT-PCR) and the method of 2-ΔΔCt (the Ct mean value of threshold cycle). 【Result】The serum GH levels of the ovariectomized goats on the 20th day showed an upward trend and were up to 9.50 μg•L-1 which were significantly higher than those of the controls (P＜0.05). On the 50th day, the levels were up to 11.55 μg•L-1 that was 2.21 μg•L-1 higher than those of the control group (9.34 μg•L-1) significantly (P＜0.01). In addition, the goats in treatment group with higher slaughter rate, lean percentage of carcass and loin eye area compared with that in the control group. The slaughter rate in ovariectomized goat was up to 45.62% which was higher than that in the control group (42.41%) and the difference was significant (P＜0.05), but the goats had a lower proportion of bone and meat (P＜0.05) in treatment group. In this study, the expression levels of GHR gene in liver of ovariectomized goats were 3.21 times (P＜0.01) more than that of the control group. Compared with the control group, a very significant increase in the GHR mRNA level was observed in longissimus of ovariectomized goats, and the expression level was the highest in the present study which was 4.87 times higher than that of the control group (P＜0.01). At the same time, a higher expression of GHR mRNA in biceps femoris (3.17 times) was also observed in ovariectomized goats, and the difference was significant compared with the control group (P＜0.05). As to perirenal fat, the expression levels of GHR gene in treatment group was 1.91 times more than that in the control group, and the difference was significant (P＜0.05)【Conclusion】 Results of the study showed that there were significant effects of ovariectomy on slaughter rate, proportion of bone and meat, serum GH levels. Meanwhile, the expression levels of GHR gene in liver, longissimus muscle, biceps femoris and perirenal fat tissues of female goats were also influenced by the ovariectomy. It is indicated that the ovariectomy increased the slaughter rate and decreased the proportion of bone and meat through enhancing the levels of serum GH and expression levels of GHR gene in the target tissues, and improved the meat performance of ovariectomized goats finally.

Key words: ovariectomy , female goats , serum GH , GHR gene

ZHANG Lei-1, WANG Yan-Yan-1, ZHOU Zhan-Qin-1, LI Guang-1, FU Ming-Zhe-1, ZHANG Suo-Liang-2, YIN Hai-Ke-2, ZHANG Sheng-Gang-2, REN Bao-Hua-2. Effects of Ovariectomy on Serum GH Levels and Expression of GHR in Some Tissues of Female Goats[J].Scientia Agricultura Sinica, 2014, 47(1): 199-208.

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