甘草酸二钾体外诱导感染MDV鸡胚成纤维细胞 凋亡发挥抗病毒作用

doi:10.3864/j.issn.0578-1752.2013.18.022

Abstract

Abstract: 【Objective】The aim of the study is to test the antiviral activity and mechanism of dipotassium glycyrrhizinate (DG) against Marek’s disease virus (MDV) in vitro.【Method】 Using MTT test and standard plaque reduction assay on chick embryo fibroblast cells (CEFs), the antiviral effect of DG against MDV was observed by antiviral assay, time-of-addition assay and virucidal assay, and the antiviral mechanism was tested by cell apoptosis assay.【Result】Antiviral assay showed that DG could reduce the damage of MDV to CEFs , EC50 was (893.5±36.99) µg•mL-1，SI＞3.36 and the inhibition ratio was 94.4%, at the maximum with no-cytotoxic concentration. Time-of-addition assay and virucidal assay demonstrated that DG could inhibit the viral replication in the full replication cycle and inactivated MDV particles in non time-dependent manner. Moreover, DG could promote MDV-infected cells apoptosis in vitro.【Conclusion】DG could inhibit MDV on CEFs by promoting MDV-infected cells apoptosis in vitro.

Key words: dipotassium glycyrrhizinate , MDV , anti-virus , cell apoptosis

CONG Xiang-Ming-1, NIU Li-1, BAI Xi-Yun-1, WU Cai-Hong-2, SUN Yao-Gui-1, HE Jun-Ping-1, BAI Yuan-Sheng-3, LI Hong-Quan-1. Dipotassium Glycyrrhizinate Against MDV on CEFs by Promoting MDV-Infected Cells Apoptosis in vitro[J].Scientia Agricultura Sinica, 2013, 46(18): 3914-3921.

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Dipotassium Glycyrrhizinate Against MDV on CEFs by Promoting MDV-Infected Cells Apoptosis in vitro

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