仿生法评定饲料干物质消化率的影响因素

doi:10.3864/j.issn.0578-1752.2013.15.014

Abstract

Abstract: 【Objective】 The objective of the present study was to determine the effects of the factors on the in vitro dry matter digestibility (DMD) of feeds using Simulative Digestion System. It would provide a method for meaning the DMD of feeds using Simulative Digestion System. 【Method】 A single factor completely randomized design with 6 treatments was adopted and each treatment contained 5 replicates with 1 digestion tube per replicate. The treatments 1-2, 3-4 and 5-6 were used to determine the effects of a preliminary method of dialysis tube, removing of the oil and fat from undigested residues, and running direction of peristaltic pump on the DMD of corn, soybean meal and wheat bran, respectively.【Result】 No significant differences were revealed on the DMD of feeds using two preliminary methods of dialysis tube (P＞0.05). Removing of the oil and fat from undigested residues, the DMD of corn, soybean meal and wheat bran were increased by 3%, and the in vitro ether extract digestibility of feeds was more than 90% (P＜0.05), but the in vitro digestibility of other nutrients was not affected by this method (P＞0.05). The DMD of feeds was different in the pushing and pulling method of running direction of peristaltic pump (P＜0.05). Although the DMD of feeds was less in the pushing method than in pulling, the results with well accuracy and precision were obtained in pushing method. 【Conclusion】The removing of the oil and fat from undigested residues and the pushing direction of peristaltic pump are suitable for the determination of DMD of feeds using Simulative Digestion System. The preliminary of dialysis tube in two methods did not significantly affect the DMD of feeds.

Key words: simulative digestion system , in vitro dry matter digestibility , preliminary method of dialysis tube , removing of the oil and fat from undigested residues , running direction of peristaltic pump

CHEN Liang, ZHANG Hong-Fu, GAO Li-Xiang, ZHAO Feng. Factors Affecting the Measure of the in vitro Dry Matter Digestibility of Feeds Using Simulative Digestion System[J].Scientia Agricultura Sinica, 2013, 46(15): 3199-3205.

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