半无叶型豌豆根瘤菌接种效应及其竞争结瘤能力分析

doi:10.3864/j.issn.0578-1752.2013.14.010

Abstract

Abstract: 【Objective】The objective of this study is to determine the effects of inoculating pea with different Rhizobium leguminosarum strains on pea’s growth and development, yield, grain quality, the competitive nodulation ability of the inoculated strains, and rhizobium strains best matching with the pea variety. 【Method】 A pot experiment was conducted by inoculating a Canadian semi-leafless pea variety 'MISER' with 9 R. leguminosarum strains (CCBAU 33109, CCBAU 43228, CCBAU 43227, CCBAU 43232, ACCC 16058, CCBAU 41242, CCBAU 43242 and 2 commercial rhizobia F98-1, F98-4) in low nitrogen soil from a cotton field in Huarong County, Hunan Province. Growing stages of pea were investigated. Pea’s main growth characteristics, nodulation and nitrogen fixation performance were measured at full-flowering stage, and meanwhile, the nodule occupancy of each inoculated strain was assayed using BOX-PCR fingerprinting technology. Pea’s main growth characteristics, yield, yield components, crude protein and crude fiber content of dry grains were determined at maturing stage. 【Result】 Compared with not-inoculated treatment (CK), the podding and pod ripening periods of pea inoculated with CCBAU 33109 were significantly increased by 4.1 and 7 d (P＜0.05), respectively. At pod ripening period, the numbers of main stem nodes of pea inoculated with ACCC 16058 were significantly increased by 2.8, 3.8 and 2.9, compared with CK and treatments inoculated with F98-1 and F98-4, respectively. The height of pea inoculated with ACCC 16058 increased significantly by 15.67% compared with F98-4. Compared with CK and the treatment inoculated with F98-1, inoculating with ACCC 16058 significantly increased MISER’s crude protein content by 9.88% and 10.63%, respectively. The grain fresh weight per plant of peas inoculated with CCBAU 41242 was also significantly 40.46% heavier than that of peas inoculated with F98-1. The nodule occupancy of CCBAU 43228 was 57.50%, higher than that of any other strain. 【Conclusion】Based on the analysis above, rhizobia CCBAU 33109, ACCC 16058, CCBAU 41242 and CCBAU 43228 matched better with the pea variety ‘MISER’ than other strains.

Key words: semi-leafless pea , Rhizobium leguminosarum , inoculation , competitive nodulation

GUO Li-Mei-12, YANG Yong-2, ZHANG Shi-Chen-2, HU Yue-Gao-2, SUI Xin-Hua-3, ZENG Zhao-Hai-2. Effects and Competitive Nodulation Ability of Inoculation with Rhizobium leguminosarum Strains on a Semi-Leafless Pea[J].Scientia Agricultura Sinica, 2013, 46(14): 2942-2952.

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Effects and Competitive Nodulation Ability of Inoculation with Rhizobium leguminosarum Strains on a Semi-Leafless Pea

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