胆碱对鹅体内脂质代谢及肝脏FAS基因mRNA表达的影响

doi:10.3864/j.issn.0578-1752.2013.13.015

Abstract

Abstract: 【Objective】 This experiment was conducted to study the effects of choline supplemented in diet on lipids metabolism and FAS mRNA expression in liver tissue of Qingnonghui geese. 【Method】 One hundred and eighty Qingnonghui geese at 1 day of age were selected and randomly divided into 6 groups with 3 replicates for each group and 10 geese for each replicate. Six groups of geese were fed the diets supplemented with 0, 600, 1 200, 1 800, 2 400 and 3 000 mg•kg-1 choline in the basal diets, respectively. The experiment lasted for fifteen weeks. The contents of liver and serum total cholesterol, triglyceride, serum low density lipoprotein cholesterol, high density lipoprotein cholesterol, blood glucose, insulin and glucagon level, liver lipoprotein lipase, hepatic lipase and serum cholinesterase activities, the gene expression of fatty acid synthetase mRNA were measured.【Result】 The results showed that at 4 weeks of age, supplementation of choline in diet significantly decreased the contents of liver and serum total cholesterol, triglyceride, serum low density lipoprotein cholesterol, insulin level, and serum cholinesterase activity (P＜0.05 or P＜0.01), and significantly increased the contents of serum glucagon and the activity of liver lipoprotein lipase and hepatic lipase (P＜0.05), but had no significant difference in contents of serum high density lipoprotein cholesterol and blood glucose (P＞0.05). At 15 weeks of age, supplementation of choline in diet significantly decreased the contents of liver and serum total cholesterol, triglyceride, serum low density lipoprotein cholesterol, insulin level and serum cholinesterase activity (P＜0.05 or P＜0.01), and significantly increased the contents of serum high density lipoprotein cholesterol, glucagon and the activities of liver lipoprotein lipase and hepatic lipase (P＜0.05), but had no significant difference in the contents of blood glucose (P＞0.05). Supplementation of choline in diet significantly increased the gene expression of fatty acid synthetase mRNA (P＜0.01), and the trend of it was decrease first and then increase, but had no significant conic relation between them (R2＜0.700). 【Conclusion】Comprehensively considering the effects of choline on lipids metabolism in liver of geese, under this experimental conditions, the optimal dietary of choline in diet is recommended as 1 200-1 800 mg•kg-1 and 1 200 mg•kg-1 for 1-4 and 5-15 weeks of age geese.

Key words: choline , Qingnonghui goose , liver lipid and blood biochemical indexes , serum hormone , FAS gene

ZHANG Wen-Xu, WANG Bao-Wei, GE Wen-Hua, ZHANG Ming-Ai, LI Wen-Li. Effects of Choline on Lipids Metabolism and FAS mRNA Expression in Liver of Qingnonghui Geese[J].Scientia Agricultura Sinica, 2013, 46(13): 2777-2787.

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Effects of Choline on Lipids Metabolism and FAS mRNA Expression in Liver of Qingnonghui Geese

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