Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (12): 2607-2614.doi: 10.3864/j.issn.0578-1752.2013.12.023

• RESEARCH NOTES • Previous Articles     Next Articles

The Virus-Free Technology of Bulblets of Chongming Daffodil (Narcissus tazetta var. chinensis) in vitro

 YANG  Liu-Yan, ZHANG  Yong-Chun, LI  Yu-Xiu, DAI  Fu-Ming   

  1. 1.Forestry and Pomology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106
    2.Shanghai Forestry Pest Control and Quarantine Station, Shanghai 200072
    3.Eco-Environment and Plant Protection Institute, Shanghai Academy of Agricultural Sciences ,Shanghai 201106
  • Received:2012-09-20 Online:2013-06-15 Published:2013-03-25

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Abstract: 【Objective】The objective of this experiment is to explore a simple-operate method to eradicate viruses of Chongming Daffodil (Narcissus tazetta var. chinensis), and detect the effect of virus-free technology.【Method】The treatments of DHT+thermotherapy and DHT+virazole were used to eliminate viruses in bulblets in vitro of Chongming Daffodil, and the effect of treatments were tested by indicator plant and RT-PCR.【Result】Thermotherapy treatment had effect on the growth and survival rate of bulblets in vitro. Thermotherapy with DHT treatment decreased the content of viruses，and when the concentration of DHT was less than 15 mg•L-1, there was less inhibitory effect on growth. Virazole at 30 mg•L-1+DHT 30 mg•L-1 had good inhibitory effect on NMaV, and there was a very low level of virus detected by RT-PCR. NDV and NCLV had higher sensitivity to two antiviral compounds, and could be mostly eradicated.【Conclusion】There was a negative effect on thermotherapy treatment. DHT with thermotherapy treatment could decrease the content of viruses detected by indicator plant. The content of NMaV in bulblets in vitro treated with 30 mg L-1DHT +30 mg L-1virazole was reduced to a very low level, and the treatment had no impact on bulblet’s survival. NDV-free and NCLV-free plants were obtained by the treatment of combination with 10 mg L-1DHT and 10 mg L-1virazole, this result was proved by RT-PCR detection.

Key words: Chongming Daffodil(Narcissus tazetta var. chinensis) , virus elimination , thermotherapy , antiviral compounds , RT-PCR detection

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