新疆南疆猪繁殖与呼吸综合征病毒分子流行病学研究

doi:10.3864/j.issn.0578-1752.2012.20.019

Abstract

Abstract: 【Objective】The objective of the study is to determine whether the porcine reproductive and respiratory syndrome virus infection is present in pig farms of South Xinjiang, and to determine the types and gene characteristics of PRRSV strain, thus pertinency effective prevention of PRRS can be made. 【Method】Seven pairs of primers were designed, and by using RT-PCR, the cloning, sequencing and sequence analysis on suspected PRRS pig tissue samples were conducted. 【Result】 There were 35 PRRSV positive in 47 test samples. The positive rate was as high as 74.47% (35/47). The 35 strains were all American type strain. There were 10 strains belong to the highly pathogenic PRRSV, accounting for 21.28% of the total tested samples (10/47), accounted for 28.57% of the positive samples(10/35); According to the comprehensive analysis, no Europe type of strains were found in the samples collected from these pig farms in this study. 【Conclusion】The American type of PRRSV strain infection and highly pathogenic strains exist in South Xinjiang.

Key words: South Xinjiang, porcine reproductive and respiratory syndrome virus （PRRSV）, molecular epidemiology

ZHAO Li, LIU Yong-Hong, JIAO Hai-Hong, ZHANG Zhi-Feng, LIU Jun-Feng, CHEN Yan-Zhou, WEN Ya-Qin, LIU Bo, LI Jiang-Tao, CUI Hao-Ran, CHENG Bo, ZHANG Chun-Guang. Molecular Epidemiology Study on Porcine Reproductive and Respiratory Syndrome Virus in South Xinjiang[J].Scientia Agricultura Sinica, 2012, 45(20): 4288-4299.

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References

[1]Cho J G, Dee S A. Porcine reproductive and respiratory syndrome virus. Theriogenology, 2006, 66(3): 655-662.

[2]Sagar M Goyal. REVIEW ARTICLE Porcine reproductive and respiratory syndrome. Journal of Veterinary Diagnostic Investigation, 1993, 5: 656-664.

[3]Baron T, Albina E, Leforban Y, Madec F, Guilmoto H, Plana Duran J, Vannier P. Report on the first outbreak of porcine reproductive and respiratory syndrome (PRRS) in France, Diagnosis and viral isolation. Annales de Recherches Veterinaires, 1992, 23(2): 161-166.

[4]Wensvoort G. Lelystad virus and porcine epidemic abortion and respiratory syndrome. Veterinary Research, 1993, 24: 117-124.

[5]Zimmerman J. Historical overview of PRRS virus. PRRS Compendium Producer Edition. National Pork Board. Des Moines, Iowa, USA, Section 1.1, 2003.

[6]赵德明, 甘孟侯, 余姗. 猪繁殖-呼吸综合征（PRRS）. 中国兽医杂志, 1996, 22（4）: 54-55.

Zhao D M, Gan M H, Yu S. Porcine reproductive and respiratory syndrome (PRRS). Chinese Journal of Veterinary Medicine, 1996, 22(4): 54-55. (in Chinese)

[7]杨汉春, 管山红, 尹晓敏, 甘梦侯. 猪繁殖与呼吸综合征病毒的分离与初步鉴定. 中国兽医杂志, 1997, 23(10): 9-10.

Yang H C, Guan S H, Yin X M, Gan M H. Isolation and identification of porcine reproductive and respiratory syndrome virus. Chinese Journal of Veterinary Medicine, 1997, 23(10): 9-10. (in Chinese)

[8]郭宝清, 陈章水, 刘文兴, 崔益洙. 从疑似PRRS流产胎儿分离PRRSV的研究. 中国畜禽传染病, 1996, 87(2): 1-5.

Guo B Q, Chen Z S, Liu W X, Cui Y Z. Porcine reproductive and respiratory syndrome virus was isolated from abortive fetus of suspected PRRS. Chinese Journal of Animal and Poultry Infectious Disease, 1996, 87(2): 1-5. (in Chinese)

[9]尹彦涛, 夏平安, 崔保安, 王建举, 党占国, 柴春霞, 陈红英, 李新生. 猪繁殖与呼吸综合征病毒河南分离株ORF3-7基因克隆与序列分析. 中国兽医学报, 2008, 28(5): 496-500.

Yin Y T, Xia P A, Cui B A, Wang J J, Dang Z G, Chai C X, Chen C X, Li X S. Cloning and sequencing of ORF3-7 genes for porcine reproductive and respiratory syndrome virus isolated in Henan province. Chinese Journal of Veterinary Science, 2008, 28(5): 496-500. (in Chinese)

[10]马伟. PRRSV-XJPRRSV1/03株结构蛋白基因的克隆与序列分析[D]. 乌鲁木齐: 新疆农业大学, 2008.

MA W. Cloning and sequences analysis of structural protein genes of PRRSV-XJPRRSV1/03[D]. Wulmuqi: Xinjiang Agricultural University, 2008. (in Chinese)

[11]Tian K G, Yu X L, Zhao T Z, Feng Y J, Cao Z, Wang C B, Hu Y, Chen X Z, Hu D M, Tian X S, Liu D, Zhang S, Deng X Y, Ding Y Q, Yang L, Zhang Y X, Xiao H X, Qiao M M, Wang B, Hou L L, Wang X Y, Yang X Y, Kang L P, Sun M, Jin P, Wang S J, Kitamura Y, Yan J H, Gao G F. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS One, 2007, 2: e526. DOI: 10.1371/journal. pone.0000526.

[12]刘永宏, 林健, 刘月焕, 王凤龙, 韩春华, 赵振华, 王金玲, 杨龙峰, 赵丽, 李栋梁, 韦海涛, 祝俊杰, 赵景义. 高致病性猪繁殖与呼吸综合征病毒感染的诊断及其分子特点. 畜牧与兽医, 2009, 41(5): 12-18.

Liu Y H, Lin J, Liu Y H, Wang F L, Han C H, Zhao Z H, Wang J L, Yang L F, Zhao L, Li D L, Wei H T, Zhu J J, Zhao J Y. Diagnosis of porcine reproductive and respiratory syndrome Virus inflection and its molecular characteristics. Animal Husbandry＆ Veterinary Medicine, 2009, 41(5): 12-18. (in Chinese)

[13]Feng Y J, Zhao T Z, Nguyen T, Inui K, Ying Ma, Nguyen T H, Nguyen V C, Liu D, Bui Q A, To L T, Wang C B, Tian K G, Gao G F. Porcine reproductive and respiratory syndrome variants,Vietnam and China, 2007. Emerging in Fectious Disease. www.cdc.gov/eid, Vol.14, November 2008,14(11): 1774-1776.

[14]Dea S, Gagnon C A, Mardassi H, Rogan D. Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: comparison of the North American and European isolates. Archives of Virology, 2000, 145(4): 659-688.

[15]Meng X J, Paul P S, Halbur P G, Morozov I. Sequence comparison of open reading frame 2 to 5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus. The Journal of General Virology, 1995, 76: 3181-3188.

[16]高志强, 郭鑫, 杨汉春. 猪繁殖与呼吸综合征病毒基因组遗传变异研究进展. 中国农业科技导报, 2002(06): 64-67.

Gao Z Q, Guo X, Yang H C. Advancement in Heredity and Variation of the Genome of Porcine Reproductive and Respiratory Syndrome Virus. Review of China Agricultural Science and Technology, 2002(06): 64-67. (in Chinese)

[17]Shibata I, Uruno K, Mori M. Serological property and replication in cell cultures of porcine reproductive and respiratory syndrome viruses isolated in Japan. The Journal of Veterinary Medical Science, 1996, 58(8): 805-807.

[18]庄金山, 袁世山, 张建武. 欧洲型PRRSV弱毒株全长基因组cDNA克隆的构建与分析. 中国兽医科学, 2008, 38(8):658-664.

Zhuang J S, Yuan S S, Zhang J W. Construction and analysis of full length genomic cDNA clone for European-type PRRSV low virulent strain. Chinese Veterinary Science, 2008, 38(8):658-664.(in Chinese)

[19]Zhou L, Zhang J L, Zeng J W, Yin S Y, Li Y H, Zheng L Y, Guo X, Ge X N, Yang H C. The 30 amino acids deletion in Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence. Journal of Virology, 2009, 83(10): 5156-5167.

[20]Han W, Wu J J, Deng X Y, Cao Z, Yu X L, Wang C B, Zhao T Z, Chen N H, Hu H H, Bin W, Hou L L, Wang L L, Tian K G, Zhang Z Q. Molecular mutations associated with the in vitro passage of virulent porcine reproductive and respiratory syndrome virus. Virus Genes, 2009, 38(2): 276-284.

[21]Wei T C, Tian Z J, Zhou Y J, An T Q, Jing Y F, Xiao Y, Hu S P, Peng J M, Hao X F, Zhang S R, Tong G Z. Evaluation of the pathogenicity of a highly pathogenic porcine reproductive and respiratory syndrome virus variant in piglets . Agricultural Sciences in China, 2011, 10(8): 1280-1291.

[22]Li Y F, Wang X L, Jiang P, Wang X F, Chen W, Wang X W, Wang K W. Genetic variation analysis of porcine reproductive and respiratory syndrome virus isolated in China from 2002 to 2007 based on ORF5. Veterinary Microbiology, 2009, 138: 150-155.

[23]Hu H X, Li X L, Zhang Z F, Shuai J B, Chen N, Liu G Q, Fang W H. Porcine reproductive and respiratory syndrome viruses predominant in southeastern China from 2004 to 2007 were from a common source and underwent further divergence. Archives of Virology, 2009, 154(3): 391-398.

[24]田克恭, 吴佳俊, 赵铁柱, 曹振, 邓小雨, 遇秀玲. 高致病性PRRSV分离株分子特征与遗传变异分析. 第三届猪病防控学术研讨会论文集, 2008: 121-127.

Tian K G, Wu J J, Zhao T Z, Cao Z, Deng X Y, Yu X L. Molecular characteristics of the highly pathogenic porcine reproductive and respiratory syndrome viruses isolates and genetic variation analysis. Conference Proceedings of the Third Pig Disease Prevention and Control, 2008:121-127. (in Chinese)

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