Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (14): 2991-2998.doi: 10.3864/j.issn.0578-1752.2012.14.023

• VETERINARY SCIENCE • Previous Articles     Next Articles

Study of the Lactobacillus Competitive Inhibition of Two Kinds of Pathogenic Bacteria Adhesion to Jejunum Epithelial Cells

 CHEN  De-Long, XU  Guang-Yong, ZHU  Hong-Liang, REN  Xiao-Ming   

  1. 北京农学院动物科学技术学院/兽医学(中医药)北京市重点实验室,北京 102206
  • Received:2012-01-10 Online:2012-07-15 Published:2012-05-21

Abstract: 【Objective】Lactobacillus acidlophilus (separated and identified by the authors’ laboratory, tentatively named LAB1) competitive inhibition of pathogenic Escherichia coli and Salmonella choleraesuis adhesion to jejunum epithelial cells (IPEC-J2) in the conditions of prevention and treatment in vitro was studied. 【Method】 IPEC-J2 cells were pretreated with LAB1 and then exposed to E. coli or S. choleraesuis which were used as simulated prevention trials. IPEC-J2 cells were exposed to E. coli or S. choleraesuis and then treated with LAB1 which were used as simulated treatment trials .Cells and three kinds of bacterial DNA were purified after bacteria adhesion to IPEC-J2 cells. The relative amount of bacteria attached was determined by amplification of the special gene relative to documented and self-designed primers, in the presence of SYBR Green. 【Result】In the simulated treatment experiment, three concentrations of LAB1 significantly inhibited 108CFU S.choleraesuis adherence to IPEC-J2 cells (P<0.05). In the simulated prevention trial, the same concentrations of LAB1 significantly inhibited S.cho leraesuisadhesion of IPEC-J2 cells (P<0.05). Simulation of the prevention and treatment trials, three concentrations of LAB1 significantly inhibited the 107CFU of E. coli adhesion to IPEC-J2 cells (P<0.05), and 109CFU LAB1 significantly inhibited the adhesion of the 109CFU of E. coli to IPEC-J2 cells (P<0.05). 【Conclusion】LAB1 have competitive inhibition on E. coli and S.cho adhesion to IPEC-J2 cells in vitro, the effect of LAB1 inhibition on E. coli and S.choleraesuis adhesion to IPEC-J2 cells in simulation of the prevention and treatment experiments is different.

Key words: FQ-PCR, Lactobacillus acidlophilus, enteric pathogens, adhesion, IPEC-J2

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