枝孢霉菌HU降解氯氰菊酯的特性及其降解产物分析

doi:10.3864/j.issn.0578-1752.2012.03.005

Abstract

Abstract: 【Objective】 The objective of this study is to optimize the biodegradation conditions and analyze the degradation products from cypermethrin degradation by Cladosporium sp. HU, and to provide a basis for bioremediation of cypermethrin- contaminated environment. 【Method】 Based on the screening of pyrethroid-degrading fungal strain, Cladosporium sp. HU (the accession number of ITS sequence analysis GenBank is HQ693526), the degradation abilities of the isolated strain under different conditions were determined with high performance liquid chromatography (HPLC). The Andrews equation was used to describe the degradation kinetics with different cypermethrin concentrations (50-500 mg•L-1). The products from cypermethrin degradation were analyzed by gas chromatopraphy-mass spectrometry (GC-MS). 【Result】 Cladosporium sp. HU utilized cypermethrin as the sole carbon for growth in the mineral salt medium (MSM). Under the conditions of aeration, inoculum amount 0.4 g•L-1, 25-30℃, pH 7.0-8.0, and shaking speed 120 r/min, Cladosporium sp. HU degraded cypermethrin rapidly with a degradation rate up to 90% within 4 d. The kinetic parameters of qmax, Ks and Ki were 1.2042 d-1, 35.2718 mg•L-1 and 439.9948 mg•L-1, respectively. And the optimal concentration of initial cypermethrin was 124.5769 mg•L-1. Cladosporium sp. HU degraded cypermethrin by hydrolysis and oxidation to produce α-hydroxy-3-phenoxy-benzeneacetonitrile, 3-phenoxybenzaldehyde, 4-phenoxyphenyl-2,2-dimethyl-propiophenone and 4-phenoxyacetophenone. 3-Phenoxybenzaldehyde and α-hydroxy-3-phenoxy- benzeneacetonitrile were the intermediates of cypermethrin degradation. 【Conclusion】 Cladosporium sp. HU participated in efficient and rapid degradation of cypermethrin, which would be in favor of fungal or enzymic preparation industrial production to deal with pyrethroid residues.

Key words: cypermethrin, Cladosporium sp. HU, degradation characteristics, degradation products

CHEN Shao-Hua, GENG Peng, HU Mei-Ying, LI Ya-Nan, CHEN Hui-Ting, CHEN Qing-Guo. Degradation Characteristics and Products of Cypermethrin by Cladosporium sp. HU[J].Scientia Agricultura Sinica, 2012, 45(3): 443-451.

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