填饲期内肥肝鹅A-FABP与MSTN基因 同步表达规律与相关性

doi:10.3864/j.issn.0578-1752.2014.03.018

摘要/Abstract

摘要： 【目的】研究与脂肪沉积有关基因在不同填饲阶段鹅组织中的表达规律及其相关性，对实施肝用型鹅新品种（系）标记辅助选择具有重要意义。重点探讨脂肪细胞型脂肪酸结合蛋白（adipocyte fatty acid-binding protein，A-FABP）基因与肌肉生长抑制素（myostatin，MSTN）基因在不同填饲日龄鹅组织中的表达规律及两基因的表达相关性。【方法】选取200只体重相近的12周龄健康青农灰鹅进行填饲。分别采集不同填饲时间里鹅肝脏、脾脏、肺脏、肾脏、胸肌、腿肌和腹脂样品，提取总RNA后，利用实时荧光定量PCR技术测定A-FABP与MSTN基因的表达量，并研究不同组织中这两个基因的表达相关性。对填饲期内肝脏重量进行研究，结合乙醚浸提法和气相色谱法测定肝脏中脂肪和总不饱和脂肪酸、总饱和脂肪酸的含量，并研究其与A-FABP与MSTN基因表达量的关系。【结果】①A-FABP基因在肝用型鹅的肝脏、腹脂、腿肌、胸肌、脾脏、肺脏和肾脏中均有表达。肝脏、腹脂、腿肌和胸肌中A-FABP基因表达量变化呈现先升高后下降的趋势，分别在填饲24、12、18和24 d达到最大值（15.90±0.04、12.72±0.12、8.88±0.06、6.33±0.13）。MSTN基因在肝用型鹅的腿肌、胸肌、肾脏、肺脏、腹脂、肝脏和脾脏中均进行了表达，MSTN基因在腿肌和胸肌中的表达呈现先升高后下降的趋势，而在肝脏、腹脂、肺脏、肾脏中MSTN基因表达量变化不显著（P＜0.01）。②鹅肝脏组织中A-FABP基因表达量与肝重、肝内脂肪、总不饱和脂肪酸及总饱和脂肪酸含量呈正相关，相关系数分别为0.576、0.510、0.463、0.501，差异均极显著（P＜0.01）；MSTN基因表达量与肝重、肝内脂肪、不饱和脂肪酸及总饱和脂肪酸含量相关性不显著（P＞0.05）。【结论】A-FABP与MSTN基因在肝用型鹅组织中的表达多呈负相关，且各自在不同填饲阶段、不同组织中的表达规律具有一定差异性，肝脏中A-FABP基因对肥肝性状的调控起一定作用。

关键词: 鹅 , 填饲 , 脂肪型脂肪酸结合蛋白 , 肌肉生长抑制素 , 基因表达 , 相关性

Abstract: 【Objective】Researches on the expression pattern of fat deposition-related genes in geese tissue are meaningful for variety selection. The expression pattern and correlationship between adipocyte fatty acid-binding protein (A-FABP) gene and myostatin (MSTN) gene in different tissues of goose during forced-feeding period were studied. 【Method】 Two hundred Qing Nong Gray geese at the age of 10 weeks were forced-fed and liver, lung, spleen, kidney, chest, leg muscle and abdominal fat during different forced-feeding periods were sampled and total RNA were extracted. The A-FABP and MSTN gene expression pattern in liver, spleen, lung, kidney, chest muscle, leg muscle and abdominal fat of geese were also evaluated using Real Time PCR. Diethyl ether extraction and gas chromatographic methods were employed to detect the content of fat and fatty acids in different tissues. Moreover, the above correlation was evaluated. 【Result】 A-FABP and MSTN genes were expressed in all tissues. A-FABP gene expression in the liver, abdominal fat, leg and chest muscles showed an up-down trend during forced-feeding period with a maximum level (15.90±0.04, 12.72±0.12, 8.88±0.06 and 6.33±0.13) at 24, 12, 18 and 24 d, respectively. MSTN gene expression pattern in the leg muscle and chest muscle showed a similar trend, while no significant difference were observed in the liver, abdominal fat, lungs and kidney (P＜0.01). A-FABP gene expression was closely related to liver weight, liver fat, total polyunsaturated fatty acids and total saturated fatty acids in goose liver. The correlation index were 0.576, 0.510, 0.463 and 0.501, respectively, with significant differences (P＜0.01), while MSTN gene expression exhibited no significant coefficients (P＞0.05). 【Conclusion】 During forced-feeding for 18-24 d, liver weight and the content of fat, total unsaturated fatty acids and total saturated fatty acids in the liver raised significantly. A-FABP and MSTN gene in different goose tissues showed different expression patterns and negative correlations were observed during forced-feeding period. A-FABP gene in goose liver plays a leading role in regulating foie gras traits.

Key words: goose , forced-feeding , A-FABP , myostatin , gene expression , correlation

王宝维, 刘腾, 葛文华, 张名爱, 李桢, 岳斌, 程凡升. 填饲期内肥肝鹅A-FABP与MSTN基因同步表达规律与相关性[J]. 中国农业科学, 2014, 47(3): 586-593.

WANG Bao-Wei, LIU Teng, GE Wen-Hua, ZHANG Ming-Ai, LI Zhen, YUE Bin, CHENG Fan-Sheng. A-FABP and MSTN Gene Expression Patterns and Their Correlation in Different Tissues of Goose During Forced-feeding Period[J]. Scientia Agricultura Sinica, 2014, 47(3): 586-593.

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