填饲鹅肝脏组织中脂肪酸沉积与FAS基因mRNA的表达丰度

doi:10.3864/j.issn.0578-1752.2012.10.013

摘要/Abstract

摘要： 【目的】研究不同填饲期肥肝鹅肝脏组织中不同脂肪酸沉积与脂肪酸合成酶（fatty acid synthetase，FAS）基因mRNA的表达丰度及相关性。【方法】对200只100日龄青农灰鹅进行填饲，从填饲0 d起，每隔6 d屠宰1次，共6次。每次随机选取10只，每只为1个重复，屠宰测定肥肝重、肝中脂肪含量、各种脂肪酸含量和FAS基因mRNA的表达丰度。【结果】①肥肝重随填饲时间的延长而增加，肝中粗脂肪含量（ether extract，EE）随肝重的增加而增加，以填饲18—24 d鹅的肥肝增重最快（504.67 g/6 d），12—18 d的次之。②肝中饱和脂肪酸（saturated fatty acid，SFA）和单不饱和脂肪酸（monounsaturated fatty acid，MUFA）的含量随着填饲时间的延长而增加，尤其是在12—18和18—24 d这两个阶段的沉积最为明显，而多不饱和脂肪酸（polyunsaturated fatty acid，PUFA）主要是在填饲后期（18—30 d）沉积；在整个填饲过程中，各种脂肪酸的沉积表现为填饲后期显著大于填饲前期（P＜0.05或P＜0.01）；填饲30 d时沉积量较大的脂肪酸为肉豆蔻酸（C14﹕0）、棕榈酸（C16﹕0）、硬脂酸（C18﹕0）、棕榈油酸（C16﹕1）、油酸（C18﹕1）、二十碳烯酸（C20﹕1）和亚油酸（C18﹕2），每100 g组织中的含量分别为0.6028 、17.72、7.25、2.75、37.42、0.3078和0.43 g。③FAS基因mRNA的表达丰度随肝脏增重和脂肪沉积速度的增加呈现出先增加后迅速降低的趋势，填饲24 d时极显著高于其它时期（P＜0.01）；0—24 d鹅肝脏组织中FAS基因mRNA表达丰度与肥肝重、EE、SFA和MUFA存在极显著正相关（P＜0.01），而与PUFA存在弱负相关且差异不显著（P＞0.05），30 d时相关性不显著（P＞0.05）。【结论】①鹅肝中EE、SFA和MUFA的含量随填饲时间和肝重的增加而增加；填饲18—24 d鹅肝脏增重和EE、SFA和MUFA的沉积速度最快；②FAS基因mRNA的表达对肥肝鹅肝脏中脂肪的沉积具有填饲前、中期快速增加，填饲后期下降的调控作用。

关键词: 填饲鹅, 肝脏, 脂肪酸, FAS基因

Abstract: 【Objective】An experiment was conducted to investigate different fatty acids deposition, FAS mRNA expression abundance and their correlation in goose liver tissue in different overfeeding periods. 【Method】 Two hundred Qingnong gray geese at the age of 100 days were selected and overfed. Ten geese were randomly selected and slaughtered every 6 days. The weight of liver, EE, the content of fatty acids and FAS mRNA expression abundance were measured. 【Result】 The weight and the fat content of liver increased with the extension of overfeeding time. They arrived to the peak during 18-24 d (504.67 g/6 d) and 12-18 d secondly. The content of SFA and MUFA in liver increased with the extension of overfeeding time, especially during 12-18 d and 18-24 d, however, PUFA deposited mainly in the late period (18-30 d). Overall, in the whole process of overfeeding, fatty acids in the late period deposited significantly (P＜0.05) or highly significantly (P＜0.01) more than the early stage. Every 100 g liver tissue has 0.6028 g myristic acid (C14﹕0), 17.72 g palmitic acid (C16﹕0), 7.25 g octadecanoic acid (C18﹕0), 2.75 g palmitoleic acid (C16﹕1), 37.42 g oleic acid (C18﹕1), 0.3078 g dodecenoicacid (C20﹕1), 0.43 g linoleic acid (C18﹕2) which were mainly deposited after overfeeding 30 days. FAS mRNA expression abundance increased to the peak at the overfeeding 24th day, then decreased quickly. During overfeeding 0-24 d, FAS mRNA expression abundance in goose liver tissue had a highly significant positive correlation with the fatty liver weight, EE, SFA and MUFA (P＜0.01), and a negative correlation with PUFA although it was not significant (P＞0.05). At the overfeeding 30th day there was no significant correlation between them (P＞0.05).【Conclusion】 The research showed that the content of EE, SFA and MUFA in liver increased with the overfeeding time and the fatty liver weight. The deposition velocity was the fastest in the period of overfeeding 18-24 d. In mRNA expression, FAS gene regulated the deposition velocity of the fat of liver tissue in fatty liver goose. It increased rapidly in the early and middle overfeeding period, however decreased in the late period.

Key words: overfeeding goose, liver, fatty acids, FAS gene

舒常平, 王宝维, 李桢, 葛文华, 张名爱, 岳斌. 填饲鹅肝脏组织中脂肪酸沉积与FAS基因mRNA的表达丰度[J]. 中国农业科学, 2012, 45(10): 2002-2011.

SHU Chang-Ping, WANG Bao-Wei, LI Zhen, GE Wen-Hua, ZHANG Ming-Ai, YUE Bin. Fatty Acids Deposition and FAS mRNA Expression Abundance in Liver Tissue of Overfeeding Goose[J]. Scientia Agricultura Sinica, 2012, 45(10): 2002-2011.

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