关键词: 鸡')">鸡, FATP1基因, 多态性, 单倍型, PCR-SSCP

Abstract:

【Objective】 The polymorphisms of FATP1 and their correlation to carcass traits in chicken were investigated for purpose of providing molecular maker information to facilitate the breeding efficiency. 【Method】 PCR-SSCP was applied to analyze the polymorphisms of partial exon loci in FATP1 gene in 381 Chinese chicken from Dahen breeding company. Genotype frequencies, allelic frequencies, correlation analysis between the polymorphisms and carcass traits were estimated. 【Result】 The result showed that 3 genotypes and 9 haplotypes combinations were found at the two loci. The genotypes of P3 loci had a significant effect on live weight, carcass weight, semi-eviscerated weight, eviscerated weight, breast weight and leg muscle weight. Different genotypes had a remarkable influence on leg muscle weight and abdominal fat percent in the P4 location. In addition, the frequencies of nine haplotypes combinations were 0.538, 0.052, 0.265, 0.029, 0.045, 0.037, 0.024, 0.005 and 0.005, and different haplotypes combinations affect the breast muscle weight, breast muscle percent and abdominal fat percent. 【Conclusion】 The correlation was detected between different genotypes and haplotypes combinations and partial carcass traits and abdominal fat percent at these two loci.

Key words: chicken')">chicken, FATP1 gene, polymorphism, haplotype, PCR-SSCP