关键词: 鹿蹄草素, 桃褐腐病菌, 多酚氧化酶, 过氧化物酶, 苯丙氨酸解氨酶, 超微结构

Abstract:

【Objective】 To explore the antifungal activity and potential mechanism of pyrolin against Monilinia fructicola. 【Method】 Agar plate tests was conducted to test the inhibitory effect in-vitro and in vivo of pyrolin. And doubling dilution method was used to determine the minimal inhibitory concentration (MIC) and minimal fugicide concentration (MFC). Polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonium-lyase (PAL) activities induced by inoculation with pyrolin and M. fructicola were studied in peach. The ultrastructure of M. fructicola treated by pyrolin was observed through scanning electron microscope (SEM) and transmission electron microscope (TEM). 【Result】 Pyrolin displayed strong antifungal activity to M. fructicola and the activity was significantly increased when the applied concentration was higher, the MIC value of 0.032 mg?mL-1 and MFC value of 0.156 mg?mL-1. The activities of PPO, POD and PAL in peach increased significantly when inoculated with pyrolin and M. fructicola, and maintained at a high level throughout the experiment. The observations with SEM revealed that the hyphae distorted, the adhesions mycelium occurred, the cell wall was rupture, cell surface occurred substance of flocculent agglutination. The observations with TEM showed that the hypha became thinning obviously, the inner structure confused, the hyphal cell was damaged severely. 【Conclusion】 Pyrolin has a strong antifungal activity and exerts a potent impact on the ultrastructure of M. fructicola.

Key words: pyrolin, Monilinia fructicola, polyphenol oxidase, peroxidase, phenylalanine ammonium-lyase, ultrastructure